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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 7 Methyl 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and DSP1-7 plasmids using Lipofectamine 3000 (Thermo Scientific) according to manufacturer’s instructions in triplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific). Expression was measured and the ΔCT was calculated ...
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bioRxiv - Biophysics 2022Quote: COS-7 cells were cultured in DMEM (GIBCO, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... and ViiA 7 Real-Time PCR machine (Thermofisher, USA) was employed ...
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bioRxiv - Cancer Biology 2023Quote: ... and 7-AAD viability staining solution (Thermo Fisher Scientific), after treatment with a human FcR blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems). Transcript RNA levels were normalized against β-actin reference gene following the 2-ΔCt method ...
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bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... blocked with 7% goat serum (Gibco; Thermo Fisher Scientific), and incubated with primary antibodies followed by incubation with fluorescent secondary antibodies (Table 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... CellEvent Caspase3/7 Green detection reagent (Thermo-Fisher Scientific) was used to label cells with caspase activity 30 minutes prior to fixation ...
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bioRxiv - Immunology 2023Quote: ... through ViiA-7 Fast Real-Time System (Applied Biosystems). Data were processed using ViiA™ 7 Software (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with 7% goat serum (Gibco; Thermo Fisher Scientific), and incubated with primary antibodies followed by incubation with fluorescent secondary antibodies (Table 2) ...
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bioRxiv - Immunology 2023Quote: ... 5 μl of 7-AAD (Invitrogen, 00-6993-50) was included for dead cell exclusion.
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bioRxiv - Microbiology 2023Quote: ... 7% Fetal bovine serum (FBS)(Cat# 26140079; ThermoFisher Scientific), and 100 U/mL penicillin-streptomycin (Cat# 15140122 ...
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bioRxiv - Genetics 2023Quote: ... A clean glass slide (Fisher Scientific, 12-544-7) was dipped into freshly made PFA solution (1% paraformaldehyde adjusted to pH 9.2 using 1.25 M sodium borate ...
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bioRxiv - Cell Biology 2024Quote: ... qPCRs were run on QuantStudio 7 (Applied Biosystems 4485701) and analyzed using the ΔΔCt method ...
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bioRxiv - Bioengineering 2024Quote: ... Huh-7 cells were cultivated in DMEM (ThermoFisher Scientific) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2024Quote: ... AT270 and HT-7 (Thermo Fisher Scientific, Waltham, MA) were used to capture/detect Tau and pTau181.
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bioRxiv - Molecular Biology 2024Quote: ... 7 μL of RNase I (Invitrogen, Cat. No. AM2249) was added to the clarified lysates and digestion was carried out for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Viability was controlled using 7-Aminoactinomycine D marker (Invitrogen). Fluorescence intensity of CTV and antibodies was measured with the SP6800 cytometer (Sony ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
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bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
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bioRxiv - Bioengineering 2024Quote: ... and HITI insert were first mixed with a ratio of 2:2:1 with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™ ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Synthetic Biology 2021Quote: ... The final plasmids were assembled in a total volume of 20 µl using 100 or 200 ng of linearized ArEc-TDH3 or GR-306MP backbone with molar insert:plasmid ratio of 7:1 (116 or 281 ng of digested PCR product, respectively) and 1 µl of T4 DNA ligase (Thermo Fisher Scientific, catalog no. EL0011). The mixtures were incubated for 3 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Claudin-4 (1:200, Thermo Fisher Scientific, 36-4800), rabbit anti-Hopx (1:100 ...
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bioRxiv - Bioengineering 2019Quote: ... overnight at 4°C followed by AlexaFluor-568 (1:500, Invitrogen, A21099 and AlexaFluor-647 antibody (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... and ethidium homodimer-1 (4 µM, stains dead cell nuclei, Invitrogen) for later timepoints.
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 1 μM Fluo-4-AM (Thermo Fisher) in culture medium and incubated at 37 °C and 5% CO2 for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... were used at 4°C overnight: anti-GFP (1:250, Invitrogen), anti-DsRed(RFP ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal anti-Claudin-4 (1:100, Novex, Life Technologies, USA) and rabbit polyclonal anti-claudin-4 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:4 dilution of trypsin was used (Gibco, Cat# 15400054) to split the cells into thirds.
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bioRxiv - Bioengineering 2022Quote: ... eBCs were stained with polyclonal Synaptotagmin-4 antibody (1:50; Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Bioengineering 2020Quote: dLNs were fixed for 1 hour with 4% paraformaldehyde (Thermofisher Scientific), embedded in Tissue-tek OCT (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... GPC-4 (#13048-1-AP, Thermo Scientific, and #MABN528, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Aliquots of 4 µL of 1 mM Mitotracker Green FM (ThermoFisher) stock solution were added to the samples and were kept in the dark at 15°C for 15–20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-FABP-4 (Thermo Fisher Scientific, PA5-30591), 1:200 rabbit anti-Osteocalcin (Thermo Fisher Scientific ...