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Citations for Thermo Fisher :
2851 - 2900 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Suspension Expi293 cells were grown to 2.5 million cell density and transiently transfected with ADAMs protease expression vectors using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific, # A14525). Medium was harvested after 6-7 days and protein was purified by Ni-NTA chromatography and assessed for quality and activity.
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
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bioRxiv - Biophysics 2022Quote: ... The PEAK3-1xFLAG construct was transfected into 30 ml of Expi293F culture at a cell density of 4.0 × 106 cells/ml using ExpiFectamine 293 transfection reagent (Thermo Fisher Scientific Cat #A14524) according to the manufacturer’s protocol at 1 μg DNA / 1 ml ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... Mutant CRP protein was produced by transfecting 25 µg plasmid DNA into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd- SARS-CoV-2-BA.5-S ...
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bioRxiv - Synthetic Biology 2023Quote: ... supernatant was harvested from 293S GnTi- TetR sender A’-C (senders 1) and 293S GnTi- TetR sender Γ’-A’ (senders 2) cells that have been continuously cultured in DMEM (41966; Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: Secretion levels from plasmid DNA constructs were evaluated following transient transfections of Expi293F™ cells using the ExpiFectamine™ 293 Transfection kit (Gibco, LifeTechnologies). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... 2×106 HEK-293 cells were electroporated with the pCMV3 vector encoding CD123 wildtype or its variants using the Neon Transfection System (ThermoFisher; 1100V, 20ms, 2pulses). To generate stable cell lines Geneticin G418 (50mg/ml ...
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bioRxiv - Immunology 2023Quote: ... After 5 days the cells were spun down at 80g for 7 minutes at 20°C and media were changed to 30 mL prewarmed Freestyle™ 293 Expression Medium with 0,5% Penicillin-Streptomycin (Thermofisher, cat no: 15140122), vortexed vigorously for 20 seconds to break cell clumps ...
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bioRxiv - Immunology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd-SARS-CoV-2-BA.5-S ...
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bioRxiv - Microbiology 2024Quote: ... Transfection of the plasmids listed in Supplementary Table 7 into Expi293F cells was performed according to the manufacturer’s instructions using the Gibco™ ExpiFectamine™ 293 Transfection Kit (Gibco™ A14524). Following transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 cells were suspension cultured in a humidified CO2 Reach-In incubator (37 °C, 5% CO2) using FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific). The cells were grown in vented cap Corning® Erlenmeyer flasks placed on Innova 2100 shaker platforms (New Brunswick Scientific ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: HEK 293T (ATCC, Manassas VA, CRL-3216) cells were grown at 37°C with 5% CO2 in DMEM (Gibco, Waltham MA, #11965-092), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Genetics 2022Quote: Human embryonic kidney cell line (HEK 293T) was purchased from the American Type Culture Collection and cultured in DMEM medium (GIBCO, Cat. NO. 12430) supplemented with 10% FBS (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Equal ratios of heavy and light chain plasmids were co-transfected into HEK 293F cells using FreeStyle MAX (Thermo Fisher Scientific, catalog #16447100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... Wild-type HEK293T and two independent clonal lines of CRISPR Serf2 KO mutant HEK 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; high glucose, pyruvate, 41966052), supplemented with 10 % Bovine Cow Serum (BCS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells were purchased from ATCC and cultured in high glucose DMEM with 2 mM GlutaMAX and pyruvate (Life Technologies catalog #10567022) supplemented with 10% heat-inactivated characterized fetal bovine serum (Hyclone ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... RPE1 and HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, high glucose, GlutaMAX™ and pyruvate supplemented (ThermoFisher Scientific, Waltham, MA, USA)) + 10% Fetal Calf Serum (FCS ...
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bioRxiv - Molecular Biology 2024Quote: HEK 293T cells stored at -80°C in freezing medium (DMEM high glucose, Sigma-Aldrich, supplemented with 20% FBS, Gibco, and 10% DMSO), were quickly defrosted at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
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bioRxiv - Biochemistry 2022Quote: ... Transient transfection was performed using expression medium comprised of a 9:1 ratio of Freestyle™293 expression medium (Thermo Fisher Scientific, Waltham MA) and EX-Cell expression medium including Glutmax (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... A Flp-In 293 cell line containing a single stably integrated FRT site at a transcriptionally active genomic locus was purchased from Invitrogen (R75007, Thermo Fisher Scientific). The FDA approved drug library was from APExBIO (L1021 ...
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bioRxiv - Bioengineering 2024Quote: ... and 3 µg of BirA-expressing plasmids were transfected into Expi293 cells using the Expifectamine 293 transfection kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A 1 mL stock of 1*10^7 cells were rapidly thawed from −80°C in 37°C water bath and transferred to 19 mL prewarmed Freestyle™ 293 Expression Medium (Thermofisher, cat no: 12338018) and incubated at 37°C at 8% CO2 under orbital shaking at 125 rpm in a 125 mL Erlenmeyer flask ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).