Labshake search
Citations for Thermo Fisher :
2801 - 2850 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The absorbance was determined at 450 nm using a microplate reader (Thermo Scientific Multiskan FC). All experiments were carried out in four biological replicates.
-
bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions were treated with anti-CD16/32 (Fc block) (14-9161-73, ThermoFisher) and stained with the live/dead marker ethidium monoazide bromide (ThermoFisher #E1374 ...
-
bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (590nm excitation and 620nm emission) with Multiskan FC (Thermo Scientific) [33 ...
-
bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (544nm excitation and 590nm emission) with Multiskan FC (Thermo Scientific) [31].
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% L-glutamine (Gibco™ 25030-024). The human nasal septum epithelial cell line RPMI 2650 (ATCC CCL-30 ...
-
bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The data was immediately collected by Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, USA) at 450 nm ...
-
bioRxiv - Plant Biology 2023Quote: ... Absorbance at 340 nm was monitored continuously with a spectrophotometer (MULTISKAN FC; Thermo Fisher Scientific) to detect NADH production.
-
bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039 ...
-
bioRxiv - Cancer Biology 2023Quote: Hap1 cells (Horizon Discovery) were maintained in IMDM supplemented with GlutaMAX and 10% FCS (Gibco). Cells regularly tested negative for mycoplasma contamination.
-
bioRxiv - Physiology 2023Quote: ... and blocked on ice for 20 minutes with Fc Block anti-CD16/CD32 (Thermo Fisher). Then ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco). Huh 7.5 cells were maintained in complete DMEM medium supplemented with non-essential amino acids.
-
bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
-
bioRxiv - Microbiology 2023Quote: ... OD600 measurement in a microplate reader (Multiskan™ FC, Thermo Scientific, Etten-Leur, the Netherlands) where performed ...
-
bioRxiv - Microbiology 2023Quote: ... Optical density was measured at 450 nanometer using a Multiskan FC machine (Thermo Scientific, USA) in combination with Mikrowin (software version 5.09 ...
-
bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
-
bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
-
bioRxiv - Immunology 2024Quote: ... Fc receptors were then blocked with anti-mouse CD16/CD32 (Invitrogen, Cat# 14-01061-86) along with extracellular staining with a mixture of conjugated antibodies in FACS buffer (1:250 for LP cells ...
-
bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) horseradish peroxidase (HRP) was purchased from Invitrogen and 3,3″,5,5″-Tetramethylbenzidine (TMB ...
-
bioRxiv - Immunology 2024Quote: ... Absorbance was detected at 450 nm by the Multiscan FC (Thermo Fisher, Waltham, MA, USA).
-
bioRxiv - Immunology 2024Quote: ... + 10% FCS + 1% GlutaMAX + penicillin-streptomycin (P/S, 12.8 U/mL, Gibco, cat.15140-122) supplemented with 10% DMSO at -150°C ...
-
bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
-
bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
-
bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
-
bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies with HRP (ab97250 ...
-
bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies, Carlsbad, California, USA) was used according to manufacturer’s instruction ...
-
bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293T/17 cells were purchased from ATCC (Cat. No. CRL-11268) were maintained in DMEM (Thermo Fisher Scientific, Cat. No. MT10013CV) with 10% fetal bovine serum and 1% Pen-Strep ...
-
bioRxiv - Immunology 2020Quote: ... The purified plasmid DNA (both heavy and light chain) was transfected into a 30mL culture of HEK Expi293 cells (Thermo Scientific Expi293). Harvesting the culture after 5 days ...
-
bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2021Quote: The two lentiviral particles of the barcode and sgRNA vectors were individually transfected into HEK-293FT cells (Cyagen, no. HEKFT-30001) with lentivirus packaging plasmids using Lipofectamine 2000 (ThermoFisher, no. 11668027). To obtain the viral particles ...
-
bioRxiv - Genomics 2022Quote: ... HEK-293T cells were grown on a 10 cm dish up to 70-80% confluency in HEK-media (KO-DMEM (Themro Fisher Scientific, 10829018), 10% fetal bovine serum (FBS ...
-
bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
-
bioRxiv - Systems Biology 2024Quote: ... VP4:VP2-complemented CVB3 A67G was prepared by transfecting HEK-293T cells with equal molar ratios of pcDNA CVB3 A67G and pcDNA3 VP4:VP2 using Lipofectamine 2000 (Thermo Fisher, 11668019). Viral supernatants were concentrated by ultracentrifugation (100,000 rcf for one hour at 4°C ...
-
bioRxiv - Microbiology 2024Quote: HEK-293T (CRL-11268) cells and PK-15cells (CCL-33) were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Carlsbad, CA USA) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2024Quote: ... was placed at the beginning of the scFc sequence to facilitate protein secretion.54 The plasmid was transfected into HEK 293F cells (Thermo Fisher Scientific). After 88 h of incubation ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in HEK-293T cells using either the Trans-Lentiviral ORF Packaging Kit (Dharmacon/Horizon Discovery) or Lipofectamine 3000 (Thermo Fisher Scientific). Viral supernatants were collected 72 hours (hrs ...
-
bioRxiv - Microbiology 2023Quote: ... scrambled or PUS1/PUS7ko HEK-293Ts were transfected with 2 μg PAN and 2 μg ORF57-eGFP plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... in the presence of the selection antibiotic when required (HEK-293T-FLAG-CB1R; zeocin at 0.22 mg/mL, Thermo Fisher Scientific #R25001), and were periodically checked for the absence of mycoplasma contamination ...
-
bioRxiv - Cell Biology 2022Quote: ... this mixture was added dropwise to confluent HEK-293T cells (ATCC, CRL-3216) in a T175 flask containing 35 mL DMEM media (Gibco #11965-092) and supplemented with 1mM sodium pyruvate (Gibco #11360-070) ...
-
bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with psPAX2 (addgene plasmid #12260) and pMD2.G (addgene plasmid #12259) into HEK 293T cells using TurboFect (Thermo Fisher Scientific). Viral supernatant was collected on day 3 and 4 after transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Wild type TGM2 and the TGM2-C277A mutant were overexpressed in HEK 293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in Dulbecco’s minimum essential medium (DMEM/F12; Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...