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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Following 2 h incubation at room temperature with an anti-rabbit peroxidase-conjugated secondary antibody (Dilution: 1:10000, Thermo Fisher Scientific, Ref: 31460) diluted in PBS pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631, 1:500 in TBST). Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380 ...
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bioRxiv - Biochemistry 2024Quote: ... anti-V5 tag (mouse monoclonal, 46-0705) and anti-V5 tag (mouse monoclonal, MA5-15253) was purchased from Invitrogen (now Thermo Fisher Scientific). anti-NUDT5 (rabbit polyclonal ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycosylation was detected by in-gel periodic acid-Schiff staining using the Pro-Q Emerald 488 glycoprotein staining kit (Invitrogen, Carlsbad, CA, USA) and imaged using a Typhoon FLA 9000 laser bed scanner (GE Healthcare Bio-Sciences ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc and DC-SIGN-Fc and then stained with Alexa Fluor® 488 goat anti-human IgG antibody (Life Technologies) diluted 1/200 in PBS + 1% FCS or BB buffer and incubated 30 min on ice ...
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bioRxiv - Genomics 2020Quote: ... cells were washed twice in FC buffer and anti-mouse Fc Alexa Fluor 647-conjugated secondary antibody (Thermo Scientific, A-21236) was added at 1:500 dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently another round of blocking with 10% FBS/ PBS at 37°C or at 4°C overnight was followed by a second round of subsequent incubation with αCSP (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and secondary anti-mouse Alexa Fluor 546 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
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bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455; IgG Fc Rabbit anti-Goat, HRP, Invitrogen 31433), and diluted in TBST with 3% nonfat dry milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... A 25pg aliquot of External RNA Controls Consortium (ERCC) RNA Spike-In Mix (Ambion, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... This spike in RNA was generated using MEGAScript T7 transcription kit (Thermo Fisher Scientific) following manufacturer’s protocols corresponding to 517 bp – 1,165 bp of the published transcript (primer sequences are detailed in Supplemental Figure 2) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with 15ug spike expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were transfected with Spike protein expressing pLP plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol and stained for their spike protein expression 72 hours after the transfection as described in Staining and Flow cytometry Analysis ...
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bioRxiv - Microbiology 2020Quote: Purified coronavirus spike ectodomains were coated onto 96-well NUNC Maxisorp plates (Thermo Scientific) at equimolar amount at room temperature (RT ...
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bioRxiv - Developmental Biology 2019Quote: ... We then added the spike-in control External RNA Control Consortium (ERCC) molecules (Invitrogen). Normalization was performed using ERCC spike in at 1:1000000 ...
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bioRxiv - Systems Biology 2019Quote: ... RNA samples were spiked with ERCC ExFold RNA spike-in mix (Life Technologies, 4456739) prior to library preparation ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of isolation spike-in mix and 1 μl of GlycoBlue Coprecipitant (Invitrogen) were added at the lysis step as described in (25) ...
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bioRxiv - Cell Biology 2019Quote: Prior to RNA isolation identical molar amounts of ERCC spike-in control RNAs (Ambion) were added to 3×105 non-induced or doxycycline induced Raji iBZLF1 (0h/6h ...
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bioRxiv - Genomics 2021Quote: ... Purified total RNA was mixed with the ERCC synthetic spike-in control (Invitrogen 4456740). RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702 ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was spiked-in with ERCC RNA Spike-In Control Mix 1 (Ambion) according to the manufacturer’s instructions prior to the start of library preparation ...
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bioRxiv - Immunology 2020Quote: ... 0.05 μl 0.5 pg/μl External RNA Controls Consortium (ERCC) spike-ins mix (Thermofisher), and 0.82 μl PCR-grade H20 (Qiagen) ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of 106 times diluted ERCC RNA spike-in control mix (Thermofisher, 4456740) was added into each denatured plasma fraction sample (i.e ...
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bioRxiv - Molecular Biology 2023Quote: ... Each sample was spiked-in with ERCC RNA Spike-In Control Mix 1 (Ambion) prior to the start of library preparation ...
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bioRxiv - Microbiology 2023Quote: ... Smart-dTV30 primer (IDT) and ERCC RNA Spike-in Mix (cat. no. 4456740, ThermoFisher) diluted 4·105 times ...
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bioRxiv - Microbiology 2023Quote: The ACE2-6xHis or spike expression plasmid was transfected into Expi293F cells (A14635, ThermoFisher) using polyethyleneimine MAX (PEI MAX ...
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bioRxiv - Genomics 2022Quote: ... we designed an experiment where the ERCC RNA Spike-In Mix (ThermoFisher, cat# 4456740) was added to RNA samples (total stool RNA from 3 donors and RNA pool 2 ...
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bioRxiv - Plant Biology 2023Quote: ... 1µL of a dilution 1:1000 of ERCC RNA Spike-In Mix (THERMOFISHER SCIENTIFIC) was added ...
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bioRxiv - Immunology 2022Quote: ... The spike protein was biotinylated using EZ-Link NHS-PEG4-Biotin reagent (Thermo Scientific) as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... 1uL of 1/100 dilution of ERCC RNA Spike-In Mix I (Thermofisher, 4456740) was added to all samples prior to library generation ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed with PBS and immersed in the solution containing the avidin- biotin-peroxidase complex (Ultra-Sensitive ABC Peroxidase Standard Staining Kit, ThermoFisher) for 30 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... Prostate cancer cell lines were cultured in RPMI medium supplemented with 2 mM L-glutamine and 10% heat-inactivated FCS (ThermoFisher Scientific, Melbourne, VIC, Australia). Cell media was changed every three days and cells were passaged regularly at ∼80-90% confluency by trypsinization ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transiently transfected with plasmids using 293fectinTM Transfection Reagent (Cat: 12347019, Life Technologies) when the cell density reached 1 × 106 cells/mL ...
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bioRxiv - Developmental Biology 2021Quote: HEK293 cells were transfected with myc or myc-MLLT11 vectors using Lipofectamine™ 2000 (Invitrogen). 24 hours later ...
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bioRxiv - Microbiology 2021Quote: ... Transfections of HEK293 were performed with 1.0 µg of expression vector using Lipofectamine 2000 (ThermoFisher). After 12 to 30 h of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... U2OS and HEK293 cells were purchased from ATCC cell repository and cultured in DMEM (Gibco), supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 and SH-SY5Y cells were transfected with 25 nM siSARM (s23032, Ambion, life technologies) for 48 h followed by 24 h rotenone treatment ...
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bioRxiv - Genetics 2019Quote: HEK293 cells used for indel acquisition assays were transfected with Liopofectamine 3000 (Thermo Fisher #L3000008) according to manufacturer’s protocol with 5ug of ErCas12a plasmid targeting each site ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM L-glutamine) and used to infect 80-100 % confluent HEK293-TRex cells (ThermoFisher) grown in 96-well plates (BD Purecoat Amine ...
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bioRxiv - Immunology 2019Quote: ... HEK293 T cells were transfected in 12-well plates with Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... For expression in the HEK293 derived Expi293F™ cells (Thermo-Fisher Scientific, Cat. No. A14527), full-length ZMPSTE24 gene was cloned into the pHTBV1.1-LIC baculovirus transfer vector (The BacMam vector backbone ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 cell line were seeded and treated with Lipofectamine 3000 (Thermo Scientific, Waltham, MA, USA) and 1µg/ml DNA in OptiMEM reduced-serum medium (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells and Mouse Embryonic Fibroblasts (MEFs) were grown in DMEM (11965-092, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 and HeLa cells were cultured at 37°C with 5% CO2 in DMEM (Gibco) with 10% heat-inactivated FBS (BioInd) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were plated in 6-well plates and maintained in high glucose DMEM (Invitrogen) growth media supplemented with 1% pen/strep (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 10% (HEK293) or 12% (C17.2 or SHSY5Y) of newborn calf serum (Thermo Fisher Scientific). Endogenous TH were depleted from the serum by stripping with activated charcoal (Sigma Aldrich ...