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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed twice with cold FACS buffer (PBS + 2% FCS) and incubated in the dark at 4° C with an anti-TfR1 (CD71) antibody (clone OKT9; Thermo Fisher Scientific, Waltham, MA, USA). Cells were then washed with PBS and resuspended in FACS buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Synthetic RNAs from the External RNAs Control Consortium (ERCC) Spike-in Mix1 (Thermo Fisher Scientific) were added at known concentrations to the same volume of RNA from the previous step ...
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Nulliparity affects the expression of a limited number of genes and pathways in Day 8 equine embryosbioRxiv - Developmental Biology 2022Quote: Five nanograms of total RNA were mixed with ERCC spike-in mix (Thermofisher Scientific, France) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μl of a 1:2,000,000 dilution of ERCC spike-ins (Invitrogen. Cat. no. 4456740) was added to each sample and mRNA was captured using modified oligo-dT biotinylated beads (Dynabeads ...
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bioRxiv - Developmental Biology 2021Quote: Five nanograms of total RNA were mixed with ERCC spike-in mix (Thermofisher Scientific, France) according to manufacturer’s recommendations ...
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bioRxiv - Biophysics 2020Quote: ... and the RaTG13 spike ectodomain (1-1209) was cloned into the pFastBac-Dual vector (Invitrogen). All the spike constructs included a C-terminal foldon tag for trimerization ...
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bioRxiv - Microbiology 2020Quote: ... The spike-in RNA mix was created by in vitro transcription (Mega-shortscript, Thermo Fisher) of PCR-amplified parts from the genomic DNA of a phiX174 bacteriophage genome (Supplementary Table S4) ...
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bioRxiv - Genetics 2019Quote: ... 100ng total RNA was spiked with ERCC Spike-In Control Mix (Ambion by Life Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 100ng total RNA was spiked with ERCC Spike-In Control Mix (Ambion by Life Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Purified total RNA was mixed with the ERCC ExFold RNA Spike-in mix (Invitrogen 4456740). RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μl of 105-fold diluted ERCC RNA spike-in mix (Thermo Fisher Scientific, 4456740) was added to each oocyte lysis sample ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 µl of ERCC RNA spike-in mix at 10:7 dilution (Life Technologies) and barcoded reverse transcription (RT ...
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bioRxiv - Genomics 2020Quote: ... 106 cells were added to a fixed amount of ERCC Spike-In RNA Mix (Invitrogen), and RNA was extracted using TRIzol reagent (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... a 97 nucleotide fragment of the spike ORF was cloned into the pJET1.2 vector (Invitrogen). Following linearization with HindIII ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... samples were spiked with equal volumes of Taqman Universal RNA Spike-In RT control (ThermoFisher) which was utilized as an internal standard ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: ... Purified total RNA was mixed with the ERCC ExFold RNA Spike-in mix (Invitrogen 4456740). RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702 ...
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bioRxiv - Biochemistry 2022Quote: ... The thiolated spike-ins were quantified with the Qubit RNA BR assay (Thermo Fisher, Q10210) and mixed at equal final concentrations ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: ... 250 ng of total RNA spiked-in with ERCC Spike-In Control Mix 1 (Ambion) as per the manufacturer’s protocol was used to generate cDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Individual samples were then labeled with isobaric tags using commercially available Tandem Mass Tag™ 6-plex (TMTsixplex™) (Thermo Fisher Scientific, P/N 90061) or TMT11plex (TMT11plex™ ...
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The fibroblasts and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (#11965–092; Gibco, NY) supplemented with 10% FBS (#12483-020 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573, RRID:CVCL_0045) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and HEK293 cells were cultured in DMEM with sodium pyruvate and GlutaMAX (Life Technologies 10569044) or 2 mM L-glutamine supplemented with 10% heat-inactivated fetal bovine serum (Sigma F4135 ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: Flp-In™ T-Rex™ HEK293 cells (Life Technologies, we did not test for mycoplasm) were cultured following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... expressing mouse ATG16L1 variants was transfected into HEK293 ATG13_KO GFP-LC3B cells via Lipofectamine 2000 (Invitrogen). Cells were selected with 2.5 μg/ml puromycin (P8833 ...
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bioRxiv - Biophysics 2020Quote: ... HEK293 cells were transfected (in 35-mm-diameter wells) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and plated onto coverslips coated with Matrigel (BD Biosciences ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: HMC3 human microglia or HEK293 cells were transfected with Lipofectamine 3000 with Plus reagent (Invitrogen L3000001) per manufacturer instructions with 0.8 μL of Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2022Quote: BiP-3xFLAG HEK293 cells (0.12 × 106) were plated on 12 mm round glass coverslips (Fisher Scientific) coated with 0.15 mg/mL poly-lysine in 24-well plates ...
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bioRxiv - Plant Biology 2022Quote: HEK293 cells were transfected with the respective eYFP-TPC1-fused constructs with lipofectamine2000 (ThermoFisher, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... siRNA duplexes (siRNA B and C) were transfected into HEK293-PSMB2-HB cells using RNAiMAX (Invitrogen) dissolved in Opti-MEM (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 (ATCC) were transfected using Lipofectamine LTX® Reagent and OptiMEM Reduced Serum Medium (Thermo Fisher) with pCMV-MAPK14 Wt (DU1765 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with relevant plasmids or siRNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific), and harvested 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... B(-)S13−) bacmids were transfected into HEK293 cells using Neon transfection system (Invitrogen, Carlsbad, CA, USA). 5 x 104 cells were resuspended in 100 μl of serum-free media containing 5ug of each mutant recombinant bacmid ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were rinsed with cold PBS then scraped in PBS+1xHALT protease/phosphatase inhibitor (ThermoFisher). The cell slurry was centrifuged for 5 min at 1,000xg at 4°C to pellet cells ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Genetics 2022Quote: ... HEK293 cell cultures were transfected with 15 μg of the plasmid DNA using Lipofectamine 2000 (Invitrogen). Transfected cells were harvested 24 hours after transfection and total RNA was isolated using RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: HEK293 and A549 cells were transfected with 15 pmol of siRNA using Lipofectamine RNAiMAX (Life Technologies). siRNAs (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells cultured in Dulbecco’s Modified Eagle medium plus GlutaMAX (DMEM, Thermo Fisher Scientific, MA, USA) supplemented with 10 % (v/v ...
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bioRxiv - Physiology 2020Quote: ... All constructs were transfected into HEK293 cells using a TurboFect transfection reagent (ThermoFisher Scientific, Waltham, MA). The day before transfection ...
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bioRxiv - Neuroscience 2021Quote: All the cells (MEF, N2a and HEK293) were plated onto tissue culture dishes in DMEM (Invitrogen) supplemented with 10% FBS and 1% Pen/Strep (Gibco ...
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bioRxiv - Biochemistry 2021Quote: Human HEK293S GnTI- cells were cultured as suspension at 37°C in FreeStyle293 expression medium (Invitrogen) supplemented with 2% (vol/vol ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were transfected with the gRNA vectors and correction template using Lipofectamine 2000 (Life Technologies) following the manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: The HEK293 cells were maintained in 15-cm-diameter plates in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% heat-inactivated (30 min at 56°C ...