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Citations for Thermo Fisher :
2851 - 2900 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:5000 in 5 % BSA in TBST or in blocking buffer (Cat. no. 37538, Thermo Fisher Scientific) in PBS (1:1 ...
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bioRxiv - Bioengineering 2022Quote: ... Tissue slices were blocked and permeabilized with a mixture of StartingBlock PBS Blocking Buffer (37538, Thermo Scientific, Waltham, MA) and 1% Triton X-100 (9002-93-1 ...
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bioRxiv - Physiology 2022Quote: ... Hearts were again washed trice in PBT (0.2% Triton X-100 in PBS) for 1h each and incubated in blocking solution (10% FBS (4133, Invitrogen), 1% BSA (A7030-10G ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 × 2 minutes and blocked with SeaBlock blocking buffer (ThermoFisher) for 15 minutes at room temperature ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: Secondary antibodies were diluted in blocking buffer at 1:2000 dilution (Alexa Fluor 594 and Alexa Fluor 488, Invitrogen), and tissues were incubated with a secondary antibody cocktail overnight at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... specimens were washed extensively with 1X PBS containing 0.5% Tween-20 (PBST) and then incubated for two hours in blocking solution (superblock (Thermofisher) supplemented with 0.05% triton X-100) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in TBS then incubated in blocking buffer containing secondary antibodies (Alexa Fluor conjugates, 1:400, Invitrogen) for 3-4 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking with 3% bovine serum albumin (BSA) in Tris-buffered saline with 0.1% Tween 20 (TBST; Thermo Fisher) for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... Slides were blocked in Universal Blocker Blocking Buffer in TBS (Pierce™ Peroxidase IHC Detection Kit, Thermo Scientific, 36000) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... then in blocking buffer and then they were incubated with an anti-rabbit AF555 secondary antibody (Thermo Fisher Scientific) and an AF488 GFP booster (Chromotek ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were prepared in 5% goat serum in blocking buffer: goat anti-mouse 594 (AlexaFluor Invitrogen, 1:2000) and goat anti-rabbit 488 (AlexaFluor Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were directly subjected to background blocking using the Ultra Vision Protein Block for 5 min (ThermoFisher Scientific) and exposed to primary and secondary antibodies for 45 min and 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 2 hours with an IFNAR1 blocking monoclonal antibody (Fisher Scientific MAR1-5A3; 10 μg/ml), or mouse IgG1 kappa isotype control ...
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bioRxiv - Neuroscience 2023Quote: ... sections were preincubated with a blocking PBS buffer containing 0.5% Triton X-100 and 10% normal horse serum (NHS; Invitrogen) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), and subsequently washed four times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer) and washed four times with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue blocking and permeabilization was subsequently performed in blocking solution (0.1 % Triton X-100 containing 10 % Normal Goat Serum (NGS, Gibco) in PBS) ...
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bioRxiv - Biophysics 2023Quote: ... Secondary antibody staining was performed for 1 hour at RT in blocking solution (AF647 anti-rabbit #A21244, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Pathology 2023Quote: Fixed FDB fibers were blocked for 2h at room temperature in Superblock™ Blocking Buffer in PBS (Thermo Scientific) with 0.04% saponin ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in TNB blocking buffer comprised of dH2O containing 0.1 M Tris-HCl (pH 7.5, Fisher Scientific), 0.15 M NaCl (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-conjugated beads were prepared by incubating antibody with beads in 300μL Blocking Buffer (PBS, 0.5% BSA [Invitrogen AM2616]) overnight at 4°C with rotation.
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bioRxiv - Immunology 2023Quote: ... All staining and blocking solutions were prepared in FACS buffer (a PBS solution with 5% fetal bovine serum (Gibco) and 0.1% sodium azide) ...
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bioRxiv - Cell Biology 2023Quote: ... The prepared samples were blocked in the blocking medium (PBS supplemented with 4% fetal bovine serum (Gibco #10500-064) and 1% bovine serum albumin (BSA)(Fisher Scientific #BPE9702-100) ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were washed again and 100 µL/well of Goat anti Rabbit IgG-HRP (1:9,000 in blocking buffer) (Invitrogen) was added and incubated for one hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Plates were removed from formalin and permeabilized with 0.2% Triton-X for 10 minutes at room temperature and treated with blocking buffer (Cell Stain Buffer; ThermoFisher). Infected cells were detected by consecutive incubation with CCHFV-specific antibody 9D5 (3 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... HUVEC samples were treated with an Fc receptor blocking reagent (1 test) (Thermo Fisher Scientific, Cat. # 14-9161-73) and incubated at 4°C for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... which involved a 30-minute incubation in a blocking buffer composed of 2.5% Normal Goat Serum (NGS) (ThermoFisher; 50062Z) and 2.5% Bovine Serum Albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue sections were then blocked with a 1x concentration blocking solution (Blocker™ BSA; #37525, ThermoFisher Scientific™) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer and developed using the SuperSignal West Femto Maximum Sensitivity Substrate kit (Thermofisher Scientific, Cat no. 34095). Membranes were imaged using FluorChem M Imager (ProteinSimple ...
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bioRxiv - Microbiology 2024Quote: ... for 10m and blocked overnight by using blocking solution (PBS plus 4% bovine serum albumin, fraction V, Fisher Scientific 9002-46-8 ...
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bioRxiv - Cancer Biology 2021Quote: Online chromatography of the extracted tryptic peptides was performed using an Ultimate 3000 HPLC system (Thermo Fisher Scientific) coupled online to a Q-Exactive-Plus mass spectrometer with a NanoFlex source (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The peptides were eluted with an acetonitrile gradient and electrosprayed into an Orbitrap Discovery mass spectrometer (Thermo Scientific) for analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Desalted peptides were labelled with TMTsixplex™ Isobaric Label Reagent Set (Catalog No. 90061, ThermoFisher Scientific, Waltham, MA) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... the peptides were loaded onto a C8 analytical column (1 mm i.d. × 50 mm length, Thermo Fisher Scientific) and separated with Agilent 1290 Infinity II UHPLC system ...
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bioRxiv - Cancer Biology 2022Quote: ... Desalted peptides were then labeled with 10-plex Tandem-mass-tag (TMT) reagents following manufacturer’s instructions (Thermo Scientific). The samples were then reconstituted in volume of 20 mM ammonium formate (pH 10 ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide mixtures were analyzed by LC-MS using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: About 500 ng of peptides were analysis using an Ultimate 3000 nanoflow liquid chromatography system (Thermo Scientific, USA) connected to a hybrid Q-Exactive HFX mass spectrometry (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The labeled sample was fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) and subject to liquid chromatography–MS/MS analysis using Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted peptides were introduced into Orbitrap Fusion Lumos system through a nanospray Flex™ ion source (Thermo Scientific) with the spray voltage of 2kV and a capillary temperature of 275°C ...
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bioRxiv - Molecular Biology 2021Quote: The peptide samples were analysed using a Lumos Orbitrap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific (Proxeon) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides eluted from the column were analysed on an Orbitrap Fusion Lumos (Thermo Fisher Scientific, San Jose, CA) mass spectrometer ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of desalted peptides were labelled immediately after being quantified with Qubit protein assay (Thermo Fisher Scientific). Multiplexed TMT samples were then fractionated using high-pH reverse phase chromatography ...
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bioRxiv - Biochemistry 2019Quote: Enriched peptides from the high pH reverse phase fractions were resolubilized in high purity water (ThermoFisher, Waltham, MA) with 0.1% formic acid (98.9% purity ...
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bioRxiv - Systems Biology 2020Quote: ... were resuspended in peptide loading buffer (0.1% FA, 0.03% TFA, 1% ACN) containing 0.2 pmol of isotopically labeled peptide calibrants (ThermoFisher 88321). Peptide mixtures were separated on a self-packed C18 (1.9 μm ...
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bioRxiv - Cell Biology 2019Quote: ... The extracted peptides were analyzed on an UltiMate 3000 RSLCnano/Q-Exactive system (Thermo Fisher Scientific, Bremen, Germany) that was set up with a Nanospray Flex ion source ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The collected peptide samples were analyzed by an Easy nLC Orbitrap Fusion Lumos platform (Thermo Fisher Scientific, USA). About 100 μg peptides were loading for the proteomic label free quantification ...
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bioRxiv - Plant Biology 2019Quote: ... The digested peptides were labeled with TMT10plex™ Isobaric Label Reagent Set (90309, Thermo Fisher Scientific, lot TE264412) and combined ...
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bioRxiv - Systems Biology 2019Quote: ... peptides were introduced by nano-electrospray into the mass spectrometer using an Easy-Spray source (Thermo Fisher Scientific) and data were acquired using MultiNotch synchronous precursor selection (SPS ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were prepared in house by mixing biotinylated (NANP)9 peptide with streptavidin conjugated PE or APC (Invitrogen) in a 4:1 molar ratio ...