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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The liganded beads were blocked with excess biotin and washed with SEA BLOCK Blocking Buffer (Thermo Fisher, Rockford, IL), 1 % BSA ...
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bioRxiv - Immunology 2021Quote: ... major (WHO/MHOM/IL/80/Friedlin) parasites were grown in Schneider’s Drosophila medium (GIBCO BRL, Grand Island, NY, USA) supplemented with 20% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Physiology 2021Quote: ... Acidified samples were desalted in C18 desalting columns (Pierce Peptide Desalting Spin Columns, Thermo Fisher Scientific, Rockford, IL, USA) and reconstituted in 95% HPLC grade water ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice with 1X BD Perm/Wash Buffer (PWB) and stained with PE-eFluor610-IL-13 (Invitrogen), Brilliant Violet 421-IL-5 (Biolegend) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1x samples were run on a 1 % Agarose gel (BP1356-500, Fisher Bioreagent, Fisher Scientific, Hanover Park, IL, USA) in 1x TAE buffer at 110 V for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... with a 30 gauge by 0.5-inch needle (305106, BD Vacutainer Labware Medical, Fisher Scientific, Hanover Park, IL, USA). Each explant was transferred into a 1.5 mL microcentrifuge tube with snap cap with as little 1x PBS as possible ...
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bioRxiv - Cancer Biology 2021Quote: ... The CRISPR IL-32 KO vector was packaged into viral particles in HEK293T cells (Open Biosystems, Thermo Fisher Scientific), using second generation packaging plasmids psPAX2 ...
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bioRxiv - Microbiology 2020Quote: The remainder of the protein was incubated with 750 µl of HisPurTM Cobalt Resin (Thermo Scientific Pierce, Rockford, IL) on a rocker for 45 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed with PBS and ECL performed (SuperSignal West Pico, or SuperSignal West Femto, Thermo Fisher Scientific Inc, IL, USA).
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bioRxiv - Microbiology 2022Quote: ... Product sizes were evaluated using gel electrophoresis (1.5% agarose) with SYBR Safe DNA Gel Stain (Fisher Scientific, Chicago, IL) and purified using a Qiagen PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... Punches were homogenized and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cells (5×106/0.5 ml) were labeled with 100 μg Immunopure Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) for 30 min at 22 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Interleukin-2 (IL-2; Clinigen, US-CA) was diluted in phosphate buffered saline (PBS; Thermo Fisher Scientific, US-CA) and added at a final concentration of 300 I.U./mL ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 100 units/mL recombinant IL-2 IS (130- 097-744, Miltenyi) and 11% horse serum (26050088, Gibco). Jurkat T cells (TIB-152 ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-10: Rn00563409_m1) for real time reverse transcriptase polymerase chain reaction were from Applied Biosystems (Foster City, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... We quantified the abundance of the peptides using the Pierce Micro BCA assay (Thermo Scientific Pierce, Rockford, IL, USA) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Antibodies used for western blotting: Primary: AHRR (rabbit polyclonal IgG AB; #PA5-107054 ; 1:250; Invitrogen, Rockford, IL, USA); CYP1B1 (rabbit polyclonal igG AB ...
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bioRxiv - Cell Biology 2024Quote: ... cells were sorted on day 57 and cultured in complete OpTmizer media supplemented with 1000U IL-2/mL (Thermofisher) for three days prior to functional analysis ...
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bioRxiv - Cell Biology 2023Quote: ... rinsed with PBS and ECL performed (SuperSignal West Pico, or SuperSignal West Femto, Thermo Fisher Scientific Inc, IL, USA).
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bioRxiv - Immunology 2022Quote: ... The bispecific antibody was purified from the collected supernatant using a protein A column (Thermo Scientific # 89960; IL, USA) and ELISA was used to assess ligand binding affinity and sensitivity of the antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... analysis was performed using TaqMan gene expression assay with ST2 and IL-1RAcP specific primers (Hs00249384_m1 and Hs00895050_m1 respectively, Life Technologies). Normalization of ST2 expression was performed using GADD45a (Hs00169255_m1 ...
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bioRxiv - Genetics 2023Quote: ... as well as editing the PAM site to protect against further edits) (Eurofins, Fisher Scientific LLC, Chicago, IL, USA). The gRNAs ...
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bioRxiv - Genomics 2023Quote: ... The LC-MS/MS system used consisted of an LTQ/Orbitrap-XL mass spectrometer (Thermo Scientific, Rockford, IL, USA) equipped with a Nanoacquity UPLC system (Waters ...
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bioRxiv - Microbiology 2023Quote: ... USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific, Rockford, IL, USA) at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: The LC was coupled to a quadrupole Orbitrap mass spectrometer (Q Exactive HFX, Thermo Fisher Scientific, Rockford, IL, USA) via a nano-electrospray ion source ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was concentrated 500-fold using 100 kDA molecular weight cut-off filters (Thermo Scientific Pierce, Rockford, IL) that were centrifuged at 2,500 x g at 4°C until appropriately concentrated ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.6) with 20 mg lysozyme and half of a crushed Pierce Protease Inhibitor Tab (Thermo Scientific, Rockford, IL) and frozen at −20°C ...
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bioRxiv - Cell Biology 2023Quote: ... A stock doxorubicin-HCl solution was initially created by dissolving doxorubicin-HCl (Fisher Scientific, Hanover Park, IL, United States) in DMSO ...
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bioRxiv - Plant Biology 2024Quote: DAPI (4′,6-diamidino-2-phenylindole) solution: Dilute 1 mg/ml DAPI (ThermoFisher, Cat. No. 62248, Rockford, IL, USA) to a final concentration of 0.5 µg/ml in 1x PBST (0.1% Tween-20).
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Microbiology 2024Quote: ... peptides were analyzed on a Q-Exactive HFX Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Neuroscience 2021Quote: ... They were then rinsed in block for >30 min at room temperature before incubation in secondary antibody (1:250 Invitrogen #A21-42 AF488 anti-mouse IgM; 1:250 Life Technologies #A11029 AF488 anti-mouse IgG1; 1:250 Invitrogen #A21141 AF488 anti-mouse IgG2b) in blocking solution for > 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and secondary antibody (anti-mouse IgG2a AF647, 1:2000, Invitrogen, A-21241; anti-mouse IgG AF568, 1:2000, Invitrogen, A-11031; anti-mouse Cy5, 1:1000), sequentially ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 anti-mouse IgG or Alexa Fluor 568 anti-mouse IgG (Thermo Fisher Scientific) at 1:500 for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse macrophage RAW 264.7 cells and mouse myoblast C2C12 cells were cultured in DMEM medium (Gibco); colorectal carcinoma COLO 741 cells and B-CLL MEC1 cells were cultured in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-mouse Alexa A647 (1:1000; goat anti-mouse IgG Alexa Fluor 647; Invitrogen #A21236) for 2h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-CLIMP63 (followed by anti-Rabbit/Mouse/goat Alexa-dye coupled antibodies (1:400, Invitrogen). SiR-lysosome was used to visualize lysosomes in live microscopy (1:2000 ...
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bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse-(H&L)-Alexa488 and Goat anti-Mouse-(H&L)-Alexa647 secondary antibodies (Invitrogen), Alexa488-conjugated phalloidin (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated in 1:1000 anti-mouse AlexaFluor488 or anti-mouse AlexaFluor594 (Thermo Fisher Scientific) and 4’,6-diamidino-2-phenylindole counterstain (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... TMEM119 (mouse primary, Biolegend, 853302, 1:250, donkey anti-mouse 488 Life Technologies, A21202, 1:1000), and CX3CR1 (PE-rat ...
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bioRxiv - Immunology 2022Quote: ... WT mouse CD8+ T cells were activated with anti-mouse CD3/CD28 dynabeads (11453D, Thermo Scientific) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently with fluorophore-labeled anti-mouse (goat anti-mouse Alexa Fluor 488; 1:2000; Invitrogen) and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse-IgG2 Alexa 568 and goat anti-mouse-IgG1 Alexa 488 (1:500, Invitrogen).
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bioRxiv - Plant Biology 2024Quote: ... Mouse monoclonal antibodies were detected with goat anti-mouse IgG HRP-linked antibody (61-6520; Invitrogen) diluted 1:5000 (v/v) ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Levels of Aβ40 in supernatants of HEK or HEKswe cells transfected with different MT5-MMP variants and/or C99 constructs or treated with C3 or DAPT were evaluated by ELISA (#KHB3481, Thermo Fisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2020Quote: ... Commercially available ELISAs for quantitative detection of human total IgG were used according to manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, U.S.A.). All plates were read in a microtiter plate reader at 450 nm and optical density values were used in statistical analyses.
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bioRxiv - Bioengineering 2020Quote: ... The blood samples were then centrifuged at 2000 xg for 10 minutes and serum collected for TNF determination by ELISA (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.