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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... were used for overnight coating of individual wells in a MaxiSorp high protein-binding capacity 96-well ELISA plate (Nunc, Denmark) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 48 h and cytoplasmic DNA was extracted according to the protocol as previously described.35 The cytoplasmic DNA lesion and plasma levels of DNA damage were measured using the DNA Damage Competitive ELISA (Thermo Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed thrice with PBS-T and 20 μL of freshly prepared SuperSignal ELISA Pico Chemiluminescent Substrate (Thermo Scientific, #37069) (mixed 1:1 ratio and diluted in equal volume with dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... ELISA wells were washed four times with PBS-T and incubated with 50 μL/well of anti-GST (Invitrogen MA4-004) or anti-MAR (Millipore-Sigma MAB1076 ...
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bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were collected for ELISA assays and RNA was extracted from infected cells using Trizol (ThermoFisher; catalog no. 15596026) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Plates were washed 5 times with washing buffer and developed by addition of 100µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with the DENV-2 NGC strain was coated onto Nunc Maxisorp ELISA plates (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Microbiology 2022Quote: ... plates were washed 3 times and then developed by adding 100 µl/well of TMB-ELISA substrate (ThermoFisher Scientific, catalog# 34029). Reactions were stopped after 10 minutes by adding 50 µl/well of 3M H2SO4 to all the wells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Venom was diluted in 100 mM carbonate-bicarbonate coating buffer pH 9.6 and coated at 100 ng per well in 96-well ELISA plates (Nunc MaxiSorp, ThermoScientific) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... plates were washed 3 times with PBST and added with 50 μL of 1-Step Ultra TMB-ELISA (ThermoFisher, Cat. #34028). Reactions were stopped by the addition of 50 μL of 2 M sulfuric acid ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 RBD antigens expressed in 293T cells were used to coat the 96-well ELISA plates (Cat No. 44240421, Thermofisher, USA). 50µL (2µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies; Grand Island ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... content in isolated mitochondria from WT and APP cells was measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer instructions (ThermoFisher khb3544).
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bioRxiv - Microbiology 2022Quote: IgG binding to SARS-CoV-2 or MeV antigens was measured by ELISA using clear flat-bottom immuno nonsterile 96-well plates (Cat# 442404, ThermoFisher Scientific) coated overnight at 4°C with 100 ng of recombinant SARS-CoV-2 proteins or 1 μg of MeV bulk antigen (Cat# BA102VS ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was taken at 590 nm with a reference filter of 620 nm using an ELISA reader (Multiskan Spectrum Microplate Reader, Thermo Scientific). All data were normalized against DMSO blank controls.
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bioRxiv - Cancer Biology 2023Quote: ... Using the standard curve from the ELISA and the amount of gDNA/sample (quantified by Nanodrop Spectrophotometer from Thermo Fisher Scientific), percentage of methylated DNA (5-mC ...
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bioRxiv - Molecular Biology 2023Quote: ... except for antibodies used for detection in ELISA and flow cytometry. Genetic constructs were synthesized by Eurofins Genomics. Plasmid DNA midiprep (cat. K210004) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... to peptides detected using DECODE was performed as follows: 12.5µL per well of 4 mM peptide solutions were coated on 384 well ELISA plates™ (Thermo Fisher Scientific) by shaking at r.t ...
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bioRxiv - Biochemistry 2024Quote: IL-6 secretion from THP-1-derived macrophages was quantified in the growth media using enzyme-linked immunosorbent assay (ELISA; Invitrogen, #BMS213INST) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... was added per well and incubated for 2-5 minutes before adding 100 µL the ELISA stop solution (Thermo Fisher Scientific). Absorbance was measured on the Varioskan microplate reader at 450 nm (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Slides were washed with PBS and incubated for four hours with anti β-actin antibody labelled with 594 fluorophore using the Zenon Mouse IgG Labeling Kit (Thermo Fisher Scientific). Dilutions of primary and secondary antibodies were the same as the ones used for the staining of human cells described above ...
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bioRxiv - Molecular Biology 2021Quote: ... Whole transcriptome sequencing was performed using 100ng of total RNA with Ion AmpliSeq Transcriptome Mouse Gene Expression kits on an Ion Proton sequencer (Life Technologies, Carlsbad, CA), with an average of 11.7 (SD ± 2.9 ...
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bioRxiv - Cell Biology 2021Quote: Neonatal mouse ventricular myocytes (NMVMs) were isolated from 1-2 day old mice following Pierce™ primary isolation kit (88281, Thermo Fisher Scientific). After 72 hours in standard culture conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were probed with species-specific donkey anti-rabbit or goat anti-mouse HRP-conjugated secondary antibody and detected with enhanced chemiluminescence (ECL) detection kit (Thermo Fisher, Carlsbad, CA).
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bioRxiv - Cancer Biology 2022Quote: Glioma cells were labeled with CellTrace reagent and injected into mouse brains as previously described (CellTrace™ Violet Cell Proliferation Kit, for flow cytometry, Thermofisher, MA, USA). A T0 measurement (maximal CellTrace intensity ...
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bioRxiv - Developmental Biology 2020Quote: All animals and pups obtained from the Spag17Pcdo mouse line were genotyped using the TaqMan® Sample-to-SNP™ kit (Applied Biosystems) for a single-nucleotide change at mouse chr1:g,100088282A>T (assay ID ANWCWCA) ...
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bioRxiv - Cell Biology 2020Quote: Cytokines and chemokines analyzed in media of primary MC cultures by multiplex bead array analysis used a FlexMap3D platform (Luminex, Austin, TX) and Cytokine Mouse Magnetic 20-Plex Panel kit (Invitrogen/ThermoFisher Scientific, Waltham, MA). The FlexMap3D results were confirmed by individual commercially available enzyme-linked immunoassay (ELISA ...
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bioRxiv - Immunology 2020Quote: ... The antibody titer of the purified UF6b-specific IgG and total IgG (serum) was determined with the Easy-Titer™ Mouse IgG assay kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Measurement of cytokine levels was performed using the Luminex Cytokine & Chemokine 36-Plex Mouse ProcartaPlex™ Panel 1A kit (Thermo Fisher Scientific, USA). Statistical significance was determined using multiple unpaired t-tests with post hoc Bonferroni’s correction for multiple comparisons.
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bioRxiv - Neuroscience 2021Quote: Whole mouse cortex was dissected from 9-month-old mouse brain and peptides were isolated using the EasyPep™ Mini MS Sample Prep Kit (Thermofisher cat# A40006) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: Primary T lymphocytes were isolated from lymph nodes of C57BL/6 mice using the Dynabeads™ Untouched™ mouse T cell kit (Invitrogen™) by negative selection ...
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bioRxiv - Neuroscience 2022Quote: ... Kit (BMK-2202) and the primary antibody was detected with Alexa-594 conjugated goat anti-mouse IgG (Invitrogen A-11005, 1:1000 dilution). Alternatively ...
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bioRxiv - Physiology 2024Quote: A minimum of four wells of mouse organoids were pooled and lysed following the protocol of the PureLink RNA Mini Kit (Ambion, Life Technologies, #12183025). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... haemoglobin RNA was removed from 1 µg of RNA extracted from blood samples using the GLOBINclear-Mouse/Rat Globin mRNA Removal Kit (Thermo Fisher Scientific; AM1981) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... We isolated CD4 T cells from pooled spleen and lymph nodes of 6-8-week-old Foxp3IRES-GFP mice using magnetic negative selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D), resting cells for at least 30 min at 4°C prior to electroporation ...
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bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration for each western blot sample was determined using BSA assays (Thermo Scientific, Rockford, IL, USA). Equal amounts of protein and varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer ...
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bioRxiv - Immunology 2022Quote: Purified IL-2 expanded MAIT cells (MAITIL-2) were stimulated for 18 hours with anti-CD3/CD28 Dynabeads (Thermofisher), IL-12 (50ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Samples were stained using 50μl of intracellular anti-cytokine antibody stain: PE-Cy7-conjugated anti-IL-13 (eBio13A, Invitrogen), PE-conjugated anti-IL-5 (TRFK5 ...
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bioRxiv - Bioengineering 2020Quote: ... AEX-HPLC analysis using Thermo Fisher Ultimate 3000 system with ProPacTM WAX-10 Columns (Thermo Scientific, Rockford, IL, USA) and RP-HPLC using Thermo Fisher Ultimate 3000 system with AdvanceBio RP-mAb Diphenyl Columns (Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Approximately 100ul of secondary antibodies diluted in 1% BSA+Triton-X were added (Thermo Fisher Scientific, Rockford, IL, USA). After incubation for 2.5 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the blots were developed using Supersignal west Pico plus Chemiluminescent substrate (Catalog No. 34580, Thermo Scientific, Rockfold, IL, USA) and then ...
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bioRxiv - Biochemistry 2021Quote: ... 5% NP-40% [Tergitol]) supplemented with 1X Halt protease and phosphatase inhibitor cocktail (from Thermo Scientific, Rockfold, IL, USA) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cancer Biology 2020Quote: ... The expression of other genes was studied using the SYBR green method (Finnzymes F410L, Thermo Scientific, Rockford, IL, USA). The Ct values were normalized with ACTB ...
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bioRxiv - Immunology 2022Quote: ... Assay ID Mm99999915_g1), and IL-1R (Cat 4453320, Assay ID Mm00434237_m1) or with Mix SYBR Green Master Mix (Applied Biosystems).