Labshake search
Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for FSTL1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... mouse IgG (ThermoFisher Scientific), goat IgG (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... Myc (Thermo Fisher, mouse). The following day ...
-
bioRxiv - Plant Biology 2020Quote: ... anti-mouse Alexa488 (Invitrogen) and anti-rabbit CY3 (Dianova)-conjugated secondary antibodies were diluted 1:600 ...
-
bioRxiv - Immunology 2020Quote: ... then anti-mouse (Invitrogen) or anti-human (Invitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse V5 (Invitrogen V8012), sheep Scp105R (from Dr ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-mouse IgG2a (ThermoFisher)] diluted 1:1000 for 1hr at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse α-GAPDH (ThermoFisher) was used at 1:10,000 final dilution ...
-
bioRxiv - Physiology 2022Quote: ... and mouse (Invitrogen, A11004) were used ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mouse-IgG (Fisher Scientific), antibodies against HA tag (Biolegend) ...
-
bioRxiv - Molecular Biology 2022Quote: ... mouse anti-GAPDH (Invitrogen)] ...
-
bioRxiv - Developmental Biology 2023Quote: ... mouse anti-GFP (Invitrogen) 1/50 ...
-
bioRxiv - Neuroscience 2023Quote: ... and mouse laminin (Invitrogen) coated 12 mm glass coverslips (Warner Instruments ...
-
bioRxiv - Physiology 2023Quote: ... SERCA1a (Thermofisher, ma3912 mouse), RyR (Thermofisher ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-H3K4me3 (Invitrogen). Secondary antibodies were purchased from Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... Mouse depletion Dynabeads (Invitrogen) were used for the depletion of lineage-labeled cells using a DynaMag-15 magnet (Invitrogen) ...
-
bioRxiv - Bioengineering 2024Quote: ... and mouse serum (Invitrogen). RQR8+CD4+ and RQR8+CD4- CAR-T cells were FACS-sorted with a Sony SH800S cell sorter into FCS ...
-
bioRxiv - Bioengineering 2024Quote: ... and mouse serum (Invitrogen). B7H3- CD34+CD4+ and B7H3- CD34+CD4- CAR-T cells were FACS-sorted with the Sony SH800S cell sorter into FCS ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse IgG (Invitrogen, 10400C), goat anti-rabbit HRP-conjugated IgG (Pierce ...
-
bioRxiv - Molecular Biology 2024Quote: ... mouse anti-Pgk1 (Thermofisher). For quantitative Western blot analyses ...
-
bioRxiv - Neuroscience 2024Quote: ... and GFP (mouse; Invitrogen). Appropriate fluorescent Alexa Fluor secondary antibodies were purchased from Invitrogen.
-
bioRxiv - Synthetic Biology 2024Quote: ... mouse monoclonal antibody (Invitrogen). Following primary antibody incubation ...
-
bioRxiv - Molecular Biology 2022Quote: Resting primary B and T cells were isolated from mouse spleens by negative selection using Mouse CD43 (Untouched™ B Cells) and Untouched Mouse T Cells Dynabeads respectively (Invitrogen). For experiments done in quiescent splenocytes ...
-
bioRxiv - Systems Biology 2020Quote: ... An Ion Torrent PGM was used with the Hi-Q 400 template preparation and sequencing kit and a 318v2 sequencing chip (Thermo Fisher, MA). All the quality control steps were performed using Bioanalyzer 2100 (Agilent ...
-
bioRxiv - Molecular Biology 2019Quote: The pFastBacNKI-his-3C-m6A-Tracer construct was used for creation of baculovirus according to the Bac-to-Bac system (Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Cell Biology 2020Quote: ... of 8 mice were then resuspended in 10 ml DMEM supplemented with 10 % heat inactivated new born calf serum (HI NCBS, LifeTechnologies / Gibco, # 26010-074) and plated on two 10 cm dishes (Corning ...
-
bioRxiv - Genetics 2021Quote: One microliter of GlobalFiler™ or GlobalFiler™ Express amplified product was added to 9.5 μL Hi-Di™ formamide (ThermoFisher Scientific) and 0.5 μL GeneScan™ 600 LIZ® size standard (ThermoFisher Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... the SBT3.8 ORF was amplified from genomic DNA with a reverse primer including six His codons and first cloned into pCR2.1-Topo (Life Technologies, Carlsbad, CA). EcoRI sites from pCR2.1 were used for ligation into pART7 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were then pooled and loaded onto chips and sequenced on Ion Torrent PGM using Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific). Fast-q or BAM files from sequencing runs were analysed in Geneious ...
-
bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
-
bioRxiv - Immunology 2019Quote: ... PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen, Carlsbad, CA, USA). CD16158F-CR and CD32131R-CR were subcloned into NcoI and MluI sites of the SFG retroviral vector ...
-
bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
-
bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
-
bioRxiv - Genetics 2019Quote: ... using 1 μl PCR product in 9.5 μl Hi-Di™ formamide containing 0.5 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
-
bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2020Quote: The Taq polymerase-amplified PCR product of the rdLRP was inserted into the TOPO Cloning site of a pcDNA3.1/V5-His-TOPO vector (Thermo Fisher Scientific, MA). The vector was transfected to 6×105 cells of HEK293T cells using Opti-MEM (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2020Quote: ... the GST-Tagged proteins were eluted with 15 mM GSH (Bio-basic) and cleaved in solution using purified recombinant His-Tagged TEV protease (Thermo Fisher Scientific). Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: The full-length OpIE2 promoter was obtained from the pIB/V5-His by PCR using Phusion High fidelity DNA Taq polymerase (ThermoFisher Scientific, USA) according to the following program ...
-
bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
-
bioRxiv - Molecular Biology 2022Quote: ... The sequencing was performed on Ion 318 v2 chip and Ion Torrent PGM machine employing the Ion PGMTM Hi-QTM View Sequencing kit (Life Technologies, USA). All procedures were carried out according to manufacturer’s instructions and each run was expected to produce approximately 150,000 reads per sample ...
-
bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
-
bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
-
bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
-
bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
-
bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
-
bioRxiv - Neuroscience 2020Quote: ... The ORF thus PCR-amplified was cloned into the pcDNA™3.1/V5-His TOPO™ TA vector (ThermoFisher Scientific, Cat # K480040) following manufacturer’s protocol.
-
bioRxiv - Evolutionary Biology 2022Quote: ... 0.5 μl of fivefold-diluted PCR product was mixed with 9.25 μl of Hi-Di formamide and 0.25 μl LIZ600 size standard (ThermoFisher Scientific, Waltham, MA, USA), denatured for 10 min at 95°C and then immediately cooled on ice ...
-
bioRxiv - Cell Biology 2019Quote: ... and FLAG-dDYNLL1 (EcoRI/XbaI) fragments were inserted into the S2-cell expression vector pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020).