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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Two microliters of each CFPS reaction were diluted with 33 μL of phosphate-buffered saline and analyzed in triplicate on a black 384-well plate with a multimode plate reader Varioskan Lux (ThermoFisher) at 488 nm excitation and 535 nm emission.
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Cell Biology 2023Quote: ... In a next step 25µl of the compound cell culture media mix were carefully transferred from the intermediate plate on plates with the cells using a Beckman Coulter Biomek Fx followed by incubation in the Cytomat incubator (ThermoFisher) at 37°C and 5% CO2 for 24 and or 72 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and the supernatants were transferred to a UHPLC-adapted 96-well plate (Nunc 96-well V-bottom polypropylene plate, ThermoFisher) and sealed ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 μM sgRNA stock plates were thawed and 5.76 μl of each guide was transferred to a 96 well conical bottom plate (Nunc, 249935) using a CyBio SELMA ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: Secreted IL-10 was determined by ELISA as per the manufacturer’s protocol (Mouse IL-10, Invitrogen, No: 88-7105-88). The color reaction was measured as OD450 units on a Bio-Rad iMark microplate reader ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: To measure the levels of secreted IL-17a from the Th17 cell culture assay we utilized the mouse IL-17a (homodimer) ELISA (ThermoFisher) according to the kit instructions.
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bioRxiv - Microbiology 2021Quote: ... Supernatants were assayed for IL-2 or IFNγ by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Thermo fisher).
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Immunology 2020Quote: ELISAs were performed using 50 ng of VP1 pentamer/well in an EIA/RIA Polystyrene High Bind Microplate (Fisher Scientific) coated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: Medium samples from cells and from the engrafted scaffolds were used to measure TGFβ using ELISA (88-8350-22, ThermoFisher), following manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Systems Biology 2024Quote: ... We detected proteins by chemiluminescence using the SuperSignal® ELISA femto maximum sensitivity substrate (Thermo Fisher Scientific™, Waltham, USA) according to the manufacturer’s instructions and the Amersham™ Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... HMGB1 was measured in cell-free supernantants using an enzyme-linked immunosorbent assay (Tecan HMGB1 ELISA, cat# ST51011, Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The plates were washed 3 times with PBS/Tween 20 0.05% (PBS-T) and saturated for 2 h at room temperature (RT) with 300 µl per well ELISA/ELISPOT diluent 1X (Invitrogen 00-4202-56 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the protein concentration was determined using the Pierce™ BCA Protein Assay Kit following the manufacturer’s instructions and a Varioskan Lux plate reader (Thermo Fisher Scientific, Waltham, MA, USA). Samples were separated using the Mini-PROTEAN® TGX™ Precast Gel 4-15% (Bio–Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Products from the second PCR were purified as described above and concentrations were normalized using SequalPrep Normalization Plate (96) Kit (Thermo Fisher Scientific, Waltham, MA, USA). We pooled and concentrated the libraries using DNA Clean and Concentrator-5 Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) in a QuantStudio 5 (384-well plate) qRT-PCR cycler (Applied Biosystems, Foster City, CA, USA). The qRT-PCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was conducted in 384 well plates using SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience, Cincinnati, OH, USA) in a QuantStudio 5 qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Microbiology 2019Quote: ... using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher), 0.2 μM of each primer and 1.5 μg of cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated PBMCs were cultured at a density of 5×105 cells/1 ml CM on 24-well plates coated with Geltrex (Thermo Fisher Scientific). After 24 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: MONHAs were seeded at 1 × 104 cells/cm2 on sterile glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 48 h ...
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bioRxiv - Cell Biology 2019Quote: ... HACAT and KB 3-1 cells were seeded into white 1536-well plates using a Multidrop Combi peristaltic dispenser (ThermoFisher, Waltham, MA) at a density of 250 ...
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bioRxiv - Immunology 2019Quote: ... Embryo carcasses were placed into individual 6 cm2 tissue culture plates containing 1 mL of pre-warmed 37°C TrypLE (Thermo Fisher, 12604013) and were incubated at 37°C for 20 min ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... 6.104 HL-1 cells were plated into each well of a 6-well plate and transduced overnight in 1 mL of transduction medium (OptiMEM-GlutaMAX, Life Technologies SAS) containing 5 µg/mL protamine sulfate in the presence of lentivectors (MOI 2) ...
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: ... and pcDNA3.1/HA-GPR17 (50 ng) or the mutant GPR17 plasmids (50 ng) per well in 96-well white plate (Thermo Scientific, Denmark). One day after transfection ...
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bioRxiv - Biophysics 2021Quote: Kinetic measurements of human erthyrocyte lysis (1%, v/v in PBS) by all ClyA mutants (at 10μM) was performed using a plate reader (Thermo Fisher - Verioscan reader) at 37◦C in shaking mode (120 RPM) ...
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bioRxiv - Neuroscience 2020Quote: ... The day before transfection, 1× 106 HEK293T cells were seeded in 10cm plate (DMEM medium supplemented with FBS and L-Glutamine, (Gibco, Ottawa, ON)) ...
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bioRxiv - Microbiology 2021Quote: The processed splenocytes were distributed at 1 x 106 per well into Nunc 96-well round bottom microwell plates (Thermo Scientific, UK). The cells were blocked by adding 5µg/ml purified rat anti-mouse CD16/CD32 (mouse BD Fc Block ...
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bioRxiv - Neuroscience 2020Quote: ... and placed in a 6-well plate filled with 1 ml/well of culture media containing 50 % Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23 % EBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The plates were then incubated with 100 µL concentration in each well mouse anti-alpaca monoclonal antibody (1:2000; Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Wild-type HeLa cells were plated in 12-well plates (200’000 cells per well) for 16 hours in 1 ml RPMI (Invitrogen, ref. no. 61870), supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... The transfected plates are rinsed in PBS and incubated in Fluorobrite medium containing NucBlue Hoescht 33342 stain (R37605, Invitrogen, 1 drop/ml). For Arl13b FRAP ...
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bioRxiv - Genomics 2022Quote: ... After 24 h the 4x 5ml input pellets were collected and 1 million cells/replicate were plated on-ADE-URA selection medium in 145 cm2 plates (Nunc, Thermo Scientific). Plates were incubated at 30 C for 7 days inside an incubator ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Neuroscience 2021Quote: ... respectively) into 24-well plates and maintained in 500 μl/well complete NDM medium (NDM + 1 μg/ml laminin (Gibco, 23017-015), 1μM cAMP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: sg-mEX3 or sg-mEX5 and SpCas9-2A-GFP plasmids at 1:1 molar ratio were co-transfected into ~80% confluence OC-k1 cells in 6-well plate using Lipofectamine 3000 (Thermo Fisher Scientific). The cells were incubated for 48 hours and then submitted for fluorescence-activated cell sorting ...