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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified antibodies were then neutralized with 14 μl of 1 M Tris-HCl pH 8.0 and buffer exchanged into PBS using Zeba Spin Desalting plates (Thermo Fisher Scientific). Purified antibodies were then stored at 4°C until use.
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bioRxiv - Immunology 2024Quote: ... The plates were washed one final time with PBST and were then developed by adding 1-step Ultra TMB (Fisher Scientific) for 60 seconds ...
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bioRxiv - Biochemistry 2024Quote: ... 0.12 x 106 COS-7 cells were seeded into each well of a 6 well plate and transfected with 1 μg total DNA using Lipofectamine p3000 (ThermoFisher Scientific). Cells were rinsed in PBS 5 hours post transfection ...
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bioRxiv - Biochemistry 2023Quote: One hundred thousand UCP1-expressing C2C12 cells were seeded on fibronectin-coated glass coverslips in 12-well plates in 1 ml of DMEM [high glucose with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Microbiology 2023Quote: ... cells were plated at 650,000 cells per well of a 6 well plate and infected with 6 dilutions from a 10-fold dilution series in 1% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Spike protein in the envelope were incubated with quadruplicates of two-fold serial dilutions of immune sera in 96-well plates prior to infection of Vero E6 cells (1×104 cells / well) in DMEM + 10% FBS (Life Technologies). At 18 hours post infection ...
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Genetics 2023Quote: ... T1 Arabidopsis transgenic plants were selected on plates with MS medium containing 15 mg l−1 hygromycin B (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Plant Biology 2023Quote: ... and 40 μL of the resulting supernatant was transferred into the well of a 1 mL 96-well NUNC plate (ThermoFisher Scientific). After adding 10 μL internal mixture (0.5 μg/mL salicylic acid-d4 and 0.5 μg/mL pipecolic acid-d9) ...
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bioRxiv - Biochemistry 2023Quote: ... Bacmid DNA containing the construct of interest was transfected into 2mL of Sf9 cells at a density of 1×106 cells/mL on a 6-well plate using ExpiFectamine Sf Transfection Reagent (Life Technologies) and following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RAD52KO and RAD52WT cells were seeded at 5 x 104 cells per well in 12-well plates with 1 mL of antibiotic-free media and treated with Lipofectamine RNAiMAX (ThermoFisher #13778075) and 10 pmol of siRNA pools (pool of 4 siRNAs ...
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bioRxiv - Biochemistry 2023Quote: Purified CHIP (1 μM) or buffer-matched control was immobilized in 96-well plates (Fisher Scientific, non-sterile, clear, flat-bottom) in CHIP buffer (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes from OT-I mice were cultured at about 1 × 106 cells/ml in 24 well-plates with RPMI (Thermo Fisher) along with 10% FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... MaxiSorp plates were coated with 1 μg/mL of monoclonal mouse anti-pig IFN-γ antibody (clone P2F6, Thermo Fisher Scientific) in carbonate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... was then transferred to a fresh six-well plate with 3mL of DMEM + 10% BCS + 1% penicillin/streptomycin (Gibco: 15070-063). Two recombinations were performed ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9-transfected U-2 OS cells (1500 cells per well of the 384-well plate) were reverse transfected with crRNAs with 1% (v/v) RNAiMAX (Thermo Fisher) in serum-free media dispensed via a Multidrop combi ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... 20 uL of cells were diluted in 1 mL of PBS within a clear 96-well microtiter plate and analyzed on the Attune NxT flow cytometer (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... on 6-well plates pre-coated with Matrigel (1:15 diluted in DMEM/F12 medium; Matrigel: Corning, #356231; DMEM/F12 medium: Gibco, 11320074) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... in a 96-well plate from 300 nm to 630 nm with 1 nm bandwidth using a Multiskan GO spectrophotometer (Thermo Scientific). An initial protein concentration of 100 μM and an initial volume of 200 μL were used for all spectrophotometric assays ...
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bioRxiv - Plant Biology 2023Quote: ... patens gametophores were grown on KNOP ME agar plates (12 g l−1 purified agar; Oxoid, Thermo Scientific, Waltham, MA, USA). Light intensity in growth cabinets was set to 70-100 µmol photons m−2 s−1 and 16:8 h light/dark cycle at 22 °C ...
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bioRxiv - Neuroscience 2023Quote: ... into low passage HEK-293T cells plated at 1×106 cells/well of a poly-D-lysine-coated 6-well plate using Lipofectamine 3000 (Life Technologies). The next day ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco, 11453D), 30U/ml hIL-2 (NIH ...
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bioRxiv - Microbiology 2023Quote: ... cholerae WT and ΔSI were diluted 1:1000 in the antimicrobial-containing MH media in 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 20 min for 24 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... cholerae WT and ΔSI were diluted 1:1000 in the antimicrobial-containing MH media in 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 20 min for 24 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were then diluted 1:1.000 in fresh medium and 200 μl were transferred into 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 15 min for 16 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at 100,000 cells per well in 12-well plates 24 h prior to transfecting 1 µg of each plasmid using Lipofectamine 3000 (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... stock was added to each sample directly before measurement of the melting temperature in a 96-well plate on a QuantStudio 1 Real-Time PCR System reader (Thermo Fisher) with a temperature increase of 0.05 °C/min ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellets corresponding to two wells of a 6-well plate (double-well pellet with same treatment conditions) were lysed in 1% (v/v) TritonX-100 buffer (Fisher Scientific) in DPBS supplemented with 1% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... 2 x 107 HEK293T cells were seeded onto 15-cm plates in DMEM supplemented with 10% (v/v) FBS and 1% (v/v) antibiotic-antimycotic (ThermoFisher Scientific). At 16 hr after seeding ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... The reaction was stopped by addition of 10 µL 10% TFA and samples were desalted by stagetipping with C18 filter plugs (Solaµ HRP 2 mg/1 ml 96 well plate, Thermo Scientific). Peptide samples were stored in 40 µL of 0.1% Formic acid at 4 °C until LC-MS analysis.
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bioRxiv - Microbiology 2023Quote: ... on confluent fibroblasts was assessed in a 96-well plate format using serial dilutions of 4EGI-1 and the alamarBlue assay as per manufacturer’s instructions (Thermo Fisher Scientific). The efficacy against Toxoplasma was determined using RH strain parasites expressing β-galactosidase in a 96-well format and measuring conversion the metabolism chlorophenol red-beta-D-galactopyranoside (as previously described (41) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 wells of a 6-well plate of mESC (corresponding to 6*10^6 cells approximately) were resuspended in 1 mL Trizol (Invitrogen,15596018) vortexed two times for 30 seconds and incubated 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... HC-04 hepatocytes (1×105) were seeded onto the bottom of a 96-well plate using IMDM (Life Technologies, 11360-070). Sporozoites were added at an MOI of 0.3 and left to invade hepatocytes for either 5 or 18 hours before being trypsinized to obtain a single cell suspension and transferred to a 96 well round bottom plate ...
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bioRxiv - Immunology 2024Quote: ... as well as transmembrane serine protease 2 (TMPRSS2) were seeded at a density of 1 × 104 cells/well in white-walled 96-well plates (ThermoFisher Scientific) one day prior to usage (day 0) ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Cell Biology 2024Quote: ... Intact spheres were subsequently diluted 1:10 in PBS and placed in a 6 well plate (Fisher Scientific, #07-000-646). Using a standard tissue culture microscope and a 20 uL pipet tip ...
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bioRxiv - Molecular Biology 2024Quote: ... HC-04 hepatocytes (1×105) were seeded onto the bottom of a 96-well plate using IMDM (Life Technologies, 11360-070). Sporozoites were added at an MOI of 0.3 and left to traverse hepatocytes for 2.5 hours in the presence of 1 mg ml-1 FITC-labelled dextran (10,000 MW ...
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bioRxiv - Microbiology 2024Quote: MDCK or MDCK SIAT1 cells were seeded in 96-well plates at 1-2 ξ 104 cells/well in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... Purified CD14+ monocytes were seeded at a density of 1 million cells/well in 6-well plates in RPMI 1640 (Life technologies) with 10% serum and 20μg/ml recombinant human MCSF (Peprotech ...
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bioRxiv - Microbiology 2024Quote: ... and added to glass coverslips in a 24-well plate along with 1 µl of Lipofectamine RNAiMAX (Thermo Fisher Scientific 13778030). After a 20 min incubation ...
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bioRxiv - Pathology 2022Quote: RNA extractions of swab material were performed in 96-well plates using Mag Max-96 AI/ND Viral RNA Isolation Kit (Applied Biosystems, Foster City, California) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 µl from prepared samples were added to one well of 96 well plate and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) manual was followed to measure the protein content ...
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... 30,000 cells were seeded into a 24-well plate (NuncMicroWell Plates with Nunclon; Thermo Fisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
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bioRxiv - Bioengineering 2021Quote: ... All experiments were performed in 384-well plates (Clear Flat-Bottom Immuno Nonsterile 384-Well Plates, MaxiSorp, Life Technologies) which were sealed (Empore Sealing Tape Pad ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal or cortical neurons were plated at 17,000 cells/well (96-well plate) or 1,000,000 cells/well (6-well plate) in neuron media (Neurobasal medium (ThermoFisher 21103049) supplemented with B27 (ThermoFisher 17504044) ...