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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 24 minutes in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 24 min in a mixture of 3% 3-aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC ethanol ...
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bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... NIM was replaced to 3:1-medium consisting of 3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Biophysics 2024Quote: ... cells were stained with DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (DiI, Invitrogen cat # D383) for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μg/mL streptomycin and 2 ng/mL mouse IL-3 (mIL-3, Gibco, Thermo Fisher). 32D cells were maintained in identical culture media with the exception of being supplemented with 15% WEHI-3B cell-conditioned media as a source of IL-3.
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bioRxiv - Biochemistry 2024Quote: ... 50 μg/mL streptomycin and 2 ng/mL mouse IL-3 (mIL-3, Gibco, Thermo Fisher). 32D cells were maintained in identical culture media with the exception of being supplemented with 15% WEHI-3B cell-conditioned media as a source of IL-3.
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bioRxiv - Developmental Biology 2024Quote: 3 dpf larvae were incubated in 3 µM FM™ 1-43 Dye (Thermofisher catalog # T3163) for 2-4 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and biotinylated at the 3’end with the Pierce RNA 3’end biotinylation kit (Thermo Fisher). Both antisense and sense strands of the DNA sequence were amplified to obtain a DNA template for in-vitro transcription (Supplementary Figure ...
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bioRxiv - Cell Biology 2024Quote: ... Media was changed every second to third day and mucus clearance was performed every 4-5 days with 5 minutes apical PBS (ThermoFisher) incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Genomics 2021Quote: Organoids seeded in 6-well plates were harvested after 3-days incubation with WYE-132 in RIPA buffer (Thermo Scientific) supplemented with protease inhibitors (Complete Mini ...
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bioRxiv - Cancer Biology 2022Quote: ... 1µM PRMT5 inhibitor or negative control treated (3 or 6 day) OCI-AML-20 cells were incubated with 10 µM EdU (Invitrogen) for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 1-O-(6-BODIPY®558/568-aminohexyl)-2-BODIPY®FL C5-sn-glycero-3-phosphocholine (Thermo Fisher Scientific) as described previously [38] ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng of nLYN-mCherry and 500 ng of iLID plasmid were mixed with 2.1 ul (1 to 3 ratio) of Fugene 6 in 125 ul of Opti-MEM medium (Thermofisher Scientific). After 10 minutes incubation at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Immunology 2022Quote: ... primers are listed in Supplemental Table 3), Luminex using a custom procarta Luminex 6-plax-cytokines (IL1β, IL6, IL8, IL18, CCL2, and TNFa) detection kit (ThermoFisher) (Supplemental Fig ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Neuroscience 2024Quote: 3 to 6 dpf-zebrafish larvae were placed in a 48-well plate (one larva per well, Fisher Scientific, USA) filled with embryo medium ...
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bioRxiv - Bioengineering 2024Quote: ... collected following 6 min of incubation with accutase and counted using an automated cell counter (Countess 3 FL, Thermo Fisher). Trypan blue was used to determine the number of live and dead cells at each evaluated timepoint ...
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bioRxiv - Microbiology 2024Quote: Colony-forming units were assayed by culturing transduced and non-transduced FL- CD34+ cells 3 days after transduction in triplicate in a 6 well plate (ThermoFisher) using complete methylcellulose (MethoCult H4435 Enriched ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were replated at a 1:3 ratio on Geltrex™-coated 6-well plates (Thermo Fisher Scientific, Cat# A1413201) and maintained in NE medium ...
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bioRxiv - Neuroscience 2024Quote: ... Correct image reconstruction and alignment for dual labeled images were assured using the X-Y coordinates of 3-6 100 nm fiducial beads (T7279, Thermofisher) as reference points to align signals with an affine/linear registration function using Localization Microscopy Analyzer (LAMA ...
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bioRxiv - Biochemistry 2024Quote: ... and Coomassie blue G-250 (0.6% w/v final concentration) were added and samples were loaded on a 3-12% Bis-Tris NativePAGE gel (Invitrogen). BN-PAGE was performed on ice according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or from ventricular tissues of various mouse hearts was minced and placed into one well of 6-well non-coated plate and cultured in 3 ml MSC medium with Normocin (Invitrogen) and Gentamicin (GIBCO).28 The media was changed daily for 3 days and then every 2-3 days ...
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bioRxiv - Cell Biology 2024Quote: COS-7 cells were grown at 37°C with 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific, USA) containing 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Primary cortical neurons were maintained for 7 days (DIV 7) before EV treatment at 37°C in 5% CO2 and cultured in serum-free Neurobasal medium (Life Technologies) supplemented with 1.2% GlutaMAX-I (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... 60 °C/15 sec and 72°C/30 sec with a final extension at 72 °C for 5 min using the ViiA 7 Real-Time PCR System equipment (Thermo Fischer/Applied Biosystems). The gene copy number was calculated based on the 2-ΔΔCT method and the gapdh gene was used as endogenous control (Table S3).
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bioRxiv - Immunology 2022Quote: About 80,000 MCF-7 cells were placed in each chamber of a 4-well chamber slide (Thermo Scientific, cat. no. 177399) containing 500 μl of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: The in vitro enzymatic assay with the fluorogenic substrate ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, UbiQ) was assembled in a 384-well microplate (Thermo Scientific). Assays were performed in 20 μL reaction volumes and in triplicate ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
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bioRxiv - Neuroscience 2023Quote: Third instar larval brains were dissected in phosphate buffered saline (PBS) and fixed with 4 % formaldehyde (FA) pH 7 (Thermo Scientific)/PBS for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and were passaged every 4-7 days by mechanical dissociation (691-ADRN) or with StemPro Accutase Cell Dissociation Reagent (Gibco, #A1110501) (691-MES) ...
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bioRxiv - Bioengineering 2022Quote: Constructs were incubated for 1 hour at 37°C with 3 mM Fluo3 AM calcium indicator (ThermoFisher Scientific) in differentiation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...