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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were stored at 4°C and mounted in SlowFade™ Gold Antifade Mountant (Thermo Fisher) within 3 days of completing the protocol.
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bioRxiv - Neuroscience 2023Quote: ... then with the primary antibodies in the same solution overnight at 4°C (SDC1, Rabbit Thermofisher MA5-32600 ...
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bioRxiv - Immunology 2023Quote: ... or Caspase-1 over-night 4°C and with fluorochrome-conjugated secondary antibody (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... and centrifuging all samples at 4°C at 6,000g for one minute (Sorvall ST89, Thermo Scientific). We then added an additional 150 µL of trizol (Tri-reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... then detected overnight at 4°C with rabbit anti-Myo10 polyclonal primary antibody (PA5-55019, Invitrogen) at a 1:500 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... An α-Mouse IgG2a-AF488 secondary antibody (Invitrogen, CAT#: A-21131; 1-4 hours, 25°C), cells were washed 3 times ...
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bioRxiv - Physiology 2024Quote: ... for 20 min at 4°C in PBS containing 2% fetal bovine serum (FBS) (10270, Gibco). Cells were then labeled with CD45 (25-0451-81 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was incubated at 4 °C for 1 h with HisPur Ni-NTA resin (ThermoFisher), after which the samples were applied twice to a gravity flow column at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-E-cadherin (1:500, overnight at 4°C, catalog no. 14-3249-82; Invitrogen), goat anti-rabbit conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2024Quote: ... plates were coated overnight at 4°C with anti-His tag mAb (Thermofisher, Cat. #PA1-983B) then washed three times with 0.2% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated at 4°C for 2 hours with Dynabeads Protein G (Invitrogen, 10003D) magnetic beads that bound anti-RNA polymerase II C-terminal domain antibody CTD4H8 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... This procedure was done in cold (4 °C) Liebovitz’s L15 cell culture media (Life Technologies, Australia). The individual vestibular neuroepithelia were further isolated ...
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bioRxiv - Molecular Biology 2024Quote: ... A quarter of samples were stored at 4°C in RNAlater (Thermo Fisher Scientific, MA, USA) for RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... The resulting pellets were soaked overnight at 4 °C in phosphate-buffered saline (PBS, ThermoFisher, 18912014) and then fully resuspended by pipetting up and down ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation in primary solution was done overnight-48hrs at 4°C on a shaker (Fisher Scientific); well plates were covered with parafilm and a plastic lid ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins were incubated overnight at 4 °C with NeutrAvidin agarose beads (Thermo Scientific) and then washed with lysis buffer three times ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hat 150 V at 4 °C before switching to light cathode buffer (Invitrogen, BN2002) to run overnight at 20-30 V at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1 mM PMSF] at 4 °C overnight using Slide-A-Lyzer (66380; Thermo Fisher Scientific). The dialyzed lysate was centrifuged at 20,400 × g at 4 °C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mixed and boiled at 95 °C with NuPage 4× LDS sample buffer (Invitrogen # NP0007) and NuPage 10x sample reducing agent (Invitrogen # NP0009) ...
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bioRxiv - Developmental Biology 2024Quote: ... Blocked tissues were incubated overnight at 4 °C with Mouse anti-FLAG (# MA1-91878 Thermo Fisher) diluted 1:1000 in NGS-PT ...
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bioRxiv - Cell Biology 2020Quote: ... Template plasmid was digested with DpnI for 2h at 37°C before amplification in Top10 competent cells (Invitrogen). Presence of S391A mutation was confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: ... Cells were passaged every 6-7 days applying 0.5 mM EDTA (Thermofisher) in sterile Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Physiology 2024Quote: ... and the QuantStudio 6 and 7 Real-Time PCR System (Applied Biosystems), using the default protocol for comparative Ct studies ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight incubation in a cold room (6-7°C) was carried and anti-mouse/rabbit secondary antibody Alexa-488 conjugated (Thermo Fisher Scientific, Waltham, MA) incubation was performed (1 hour ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ul of each sample was loaded on 4-12% Bis-Tris gels (Thermofisher).
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bioRxiv - Molecular Biology 2021Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside in DMSO (0.1-1mM, AICAR, Fisher Scientific), or isoproterenol (5-25µM ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen) and transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min and loaded onto 4%–12% Nupage Bis-Tris gels (Novex/Life Technologies). Proteins were transferred using the iBlot2 (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days by treatment with StemPro Accutase (ThermoFisher, Cat#A1110501), stored in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the 11.5 μL RNA were supplemented with 4 μL 5× first strand buffer (ThermoFisher Scientific), 1 μL 10 μM reverse primer ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 minutes and loaded onto NuPAGE 4-12% Bis-Tris Midi Gels (Invitrogen, WG1403BOX) and run at 140V for approximately 1.5 hrs ...
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bioRxiv - Microbiology 2024Quote: ... 20 mM glucose) before being loaded with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Each iWAT and eWAT depot received 4–5 μL of 0.1% CTB-488 (Invitrogen C34775) or CTB-647 (Invitrogen C34778 ...
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bioRxiv - Cell Biology 2024Quote: ... On DIV 4-5 medium was exchanged for phenol red-free Neurobasal medium (Thermo Fisher) supplemented with GlutaMAX 1x (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: hESCs were routinely passaged every 4–5 days by dissociating in Accutase (ThermoFisher Scientific, A1110501) for 3 minutes at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 5-10µg of lysate was ran on 4-12% NuPAGE Bis-Tris Gels (ThermoFisher), transferred to PVDF membranes ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were probed with the following primary antibodies at 4°C overnight: mouse anti-EphA4 C-terminus (Invitrogen, USA; 1:1000), goat anti-EphA4 N-terminus (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples are frozen for at least 10 min at −80°C and then immediately centrifuged for 90 min at 4°C and 13000 rpm (Heraeus™ Biofuge fresco, Thermo Scientific). After centrifugation three different stable phases are obtained ...
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bioRxiv - Neuroscience 2023Quote: ... We cooled water to 4 °C (Yeosen, PH-F3) and heated water to ∼55 °C using a hot plate (Fisher Scientific, HP88854200) set to 170 °C ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at 4°C overnight and then blocked for 1 hour at 37°C using SuperBlock (ThermoFisher Scientific, Cat. # 37516), and then washed four times with PBS 0.05% Tween-20 (PBST) ...
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bioRxiv - Plant Biology 2023Quote: ... Extracted protein samples were centrifuged at 20,000 g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the Pierce™ Anti-c-Myc Magnetic Beads (88843; Thermo-Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Media was changed every second to third day and mucus clearance was performed every 4-5 days with 5 minutes apical PBS (ThermoFisher) incubation ...
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bioRxiv - Cell Biology 2022Quote: ... they were concentrated from 40 μl to 5 μl during 2h at low temperature using speedvac (Thermofisher). Small RNA libraries were then built with Illumina TruSeq Small RNA Sample kit following manufacturer’s recommendations ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...