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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or PureLink™ HiPure Plasmid Miniprep Kit (Art. No. K210011, Invitrogen™), eluted in sterile water and confirmed by enzymatic digestion and sequencing.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HEK293 cells using either Lipofectamine 2000 reagent (Invitrogen) or calcium phosphate transfection.
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bioRxiv - Biochemistry 2022Quote: ... These entry plasmids were transposed to the destination vector (pDEST8, Invitrogen, USA) with Gateway LR reaction according to the manufacture’s instruction for construct transfer plasmids ...
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bioRxiv - Neuroscience 2022Quote: ... Products were purified using the GeneJet Plasmid Mini Prep Kit (Thermo scientific).
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA was transformed into TOP10 chemically competent cells (Invitrogen, c4040-10) by the heat-shock method ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were co-transfected into cells using Lipofectamine 3000 reagent (Thermo Fisher). The cell medium was replaced with fresh DMEM medium (containing 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... by cutting the plasmids containing the ATS variant with PmeI (ThermoFisher Scientific), which introduces two cuts and exposes homology regions to the MET15 locus ...
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bioRxiv - Biochemistry 2022Quote: ... The PLP/DM20 plasmid was transformed into DH10Bac strain (Thermo Fisher Scientific) for bacmid production ...
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bioRxiv - Molecular Biology 2022Quote: ... coli DH5α cells using a GeneJET Plasmid Miniprep kit (ThermoFisher Scientific K0503). Plasmids were generated by standard restriction digests ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2.5 µg pVSV-G plasmid with 10 µL Lipofectamine 2000 (Invitrogen) diluted in 4 mL Opti-MEM ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid concentrations were determined using a Quant-iT PicoGreen dsDNA kit (Invitrogen), and 200 ng of each plasmid was electroporated into 2 ODU of L ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific) and transformed into NEB 5-alpha Competent Escherichia coli (High Efficiency ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μg plasmid was mixed with 1.5 μl Lipofectamine 3000 (Life Technologies) and 2 μl P3000 reagent (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the CrPV or the minigenomes were linearized with Ecl136ii (Thermofisher) or Eco53KI (NEB) ...
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bioRxiv - Immunology 2022Quote: ... and the plasmid was recirculated by T4 DNA ligase (Thermo Fisher Scientific). The CAR constructs were transfected to Plat-A packaging cell line via Lipofectamine 3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Five micrograms of linearised plasmid template were pre-treated with RNAsecure (ThermoFisher) and used in a 50μl transcription reaction containing 5 x transcription buffer (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids were transformed into Escherichia coli BL21(DE3)pLysS cells (Invitrogen), and cells harboring the plasmids were grown at 37°C until the OD600 reached 0.7-1.0 in Luria Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... coli by the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA) and modified or used for full-length genome assembly.
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bioRxiv - Microbiology 2022Quote: ... coli using the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA). Table S3 lists the primers used for the construction of the variant S DNA fragments.
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were cloned and propagated in Mach I cells (Thermo Fisher Scientific) with the exception of pHelper ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The plasmids were purified by a PureLink miniprep kit (Thermo Fisher Scientific) and outsourced for Sanger sequencing (HyLabs ...
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bioRxiv - Plant Biology 2022Quote: ... The bait and prey plasmids were transformed into yeast strain MaV203 (Invitrogen): MATα leu2-3,112 trp1-901 his3Δ200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Co-transfection of the plasmids was then performed using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: PX459-hRABL3 plasmid was transfected into RPE1 cells using Lipofectamine 2000 (Invitrogen). After 24 hrs ...
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bioRxiv - Microbiology 2021Quote: ... 10 μg of each CHIKV plasmid was linearized overnight using NotI (Invitrogen), purified by phenol:chloroform extraction and used as a template for in vitro transcription using the SP6 mMessage mMachine kit (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids were transformed into Agrobacterium tumefaciens GV3201 (Thermo Scientific, Waltham, MA, US) and selected using 50 mg/L spectinomycin and 50 mg/L gentamycin to maintain the pMP90 plasmid (MP Biomedicals Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... The ATRX plasmids were propagated using ElectroMAXTM Stbl4TM Competent cells (Life Technologies). To generate Flag-tagged DAXX expression plasmid (pCDH-Flag-DAXX-Puro) ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA transfection was performed with the LT-X transfection reagent (Invitrogen). Transfected cells were plated on poly-D-lysine (Sigma-Aldrich)−coated borosilicate cover glass (Paul Marienfeld ...
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bioRxiv - Genetics 2021Quote: ... The plasmids were used to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected using Lipofectamine 2000 according to the manufacturer’s instructions (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of pUC19-piggyBAC transposase plasmid using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... or pcDNA3.2/V5-DEST (control) plasmid using Lipofectamine2000 (Thermo Fisher Scientific, #11668500) After 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with plasmid DNA using Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: Transient transfections of reporter plasmids were carried out using Lipofectamine 3000 (Invitrogen) when co-transfections with miRNA mimics or plasmids for ectopic expression of hMOV10 were assayed for retrotransposition ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cells were resuspended in 200 μL GeneJET Plasmid MiniPrep (ThermoFisher Scientific) resuspension buffer supplemented with 3 μL 1000 U/mL Zymolase (Amsbio ...
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bioRxiv - Cell Biology 2022Quote: ... The dual expression plasmid was transformed into the Rosetta II (DE3) (Invitrogen) E ...
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bioRxiv - Cancer Biology 2022Quote: ... All plasmids were grown up in Top10 competent bacteria (Invitrogen, Waltham, MA) and plasmid DNA prepped from bacteria with Qiagen DNA mini prep or maxi prep kits Germantown ...
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bioRxiv - Molecular Biology 2022Quote: ... 1-2 μg of Plasmid DNA along with transfection agents Lipofectamine (Invitrogen) or Polyetheleneimine ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid construction and amplification were performed in the bacteria strain TOP10 (Thermofisher Scientific ...
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bioRxiv - Biophysics 2022Quote: Cells were transfected with plasmid DNA using Lipofectamine 3000 reagent (Thermofisher #L3000001) transiently or stably ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid DNA (1 µg/ml culture) and ExpiFectamine™ 293 Reagent (Gibco) were diluted in Opti-MEM® I medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... and purified using PureLink HQ Mini Plasmid DNA (Life Technologies Ltd, K210001).
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bioRxiv - Biophysics 2019Quote: ... plasmids were obtained from the GeneArt Gene Synthesis Service (Thermo Fisher Scientific). Template DNA and primer design were performed with assistance of the molecular biology module of the Benchling platform.
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were transfected with plasmid encoding ACE2 with Lipofectamine 2000 (Invitrogen). 48 h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli (DH5α) with an endotoxin-free plasmid extraction kit (Invitrogen, Carlsbad, CA) and transfected into ExpiCHO-S cells using ExpiFectamine Transfection Kit (Gibco) ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Amplicons containing the attB sites were recombined into pDONR 221 plasmids (Invitrogen). They were then transferred into the binary vector pGWB415 (Nakagawa et al. ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Amplicons containing the attB sites were recombined into pDONR 221 plasmids (Invitrogen). They were then transferred into the binary vector pK7FWG2 (Karimi et al. ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Amplicons containing the attB sites were recombined into pDONR Zeo plasmids (Invitrogen). They were then transferred into the binary vector pK7WGF2 ...
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bioRxiv - Microbiology 2020Quote: The resulting plasmid pBSJL2-cat was digested by Fastdigest BamHI (Thermo scientific) and the band obtained was purified from agarose gel using QIAquick gel extraction kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinant plasmids were transformed into INVSc1 Saccharomyces cerevisiae (Thermo Fisher Scientific) using the PEG-LiAc method ...