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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... coli and extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The construction was verified using enzymatic digestion with KpnI and PvuI.
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cultures using PureLink HiPure Plasmid Midiprep Kit (Invitrogen, Cat. No. K210005). To facilitate DNA delivery to ESCs through nucleofection ...
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bioRxiv - Plant Biology 2022Quote: ... the linearized plasmid was digested with 20 U DNase I (Thermo Scientific) for 10 min at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid assembly was performed by ligation with T4 DNA ligase (Thermo Scientific) or by the Gibson reaction using the NEBuilder HiFi assembly master mix (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5 μg pVSV-G plasmid with 10 μL Lipofectamine 2000 (Invitrogen) diluted in 4 mL Opti-MEM ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The plasmids were transfected with Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA) and siRNAs transfected with RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Positive clones were confirmed by plasmid sequencing (3130 Genetic Analyzer, Applied Biosystems). Resulting sequences were analyzed using Sequence Scanner v 2.0 (Applied Biosystems) ...
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Capacity to erase gene occlusion is a defining feature distinguishing naive from primed pluripotencybioRxiv - Molecular Biology 2021Quote: ... PCR products were cloned into plasmid using TOPO TA Cloning Kit (Invitrogen) and individual clones were picked for Sanger sequencing of the inserts.
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bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA was mixed with calf thymus carrier DNA (Thermo Fisher Scientific) to give a total of 10 μg DNA ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were pre-complexed with 3 µg lipofectamine 2000 (Life Technologies 11668019) in 200 µl Opti-MEM (Life Technologies 51985091 ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... The entire construct was cloned into a pJet1.2 plasmid backbone (Thermo Fisher) using Gibson assembly master mix (NEB)79 ...
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bioRxiv - Synthetic Biology 2020Quote: ... cultures were prepared using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Part plasmids were verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmid DNA/siRNA and lipofectamine were individually diluted in Optimem (Gibco) and incubated for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and GeneJet gel or plasmid purification kits were purchased from Thermo Scientific. Endotoxin-free plasmid DNA isolation kits were purchased from Qiagen (cat ...
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bioRxiv - Molecular Biology 2019Quote: The Eco72IM sequence was PCR-amplified from the Eco72IRM plasmid (Thermo Fisher) and recombined with the pDONR 221 (Gateway ...
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bioRxiv - Developmental Biology 2020Quote: ... HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (Life Technologies). After 48h ...
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bioRxiv - Molecular Biology 2019Quote: ... plated and extracted using GeneJET Plasmid Midiprep Kit (Thermo Fisher Scientific, USA). Number of colonies was estimated to be around 10000 individual clones.
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bioRxiv - Genetics 2019Quote: ... Plasmids were transfected into podocytes or HEK293 cells using Lipofectamine 2000 (Invitrogen). HEK293 cells stably expressing p3xFLAG-ADCK4 or BAP were selected and maintained with 1 mg/mL G418.
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bioRxiv - Molecular Biology 2019Quote: Three incisions-bearing plasmid (∼10 μg) was column purified (Invitrogen, cat#K220001) to check for digestion ...
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bioRxiv - Genomics 2020Quote: ... G8P plasmid (1 μg) were transfected into cells using Lipofectamine 3000 (Invitrogen). At 24 h post G8P transfection ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid transfections and siRNA knock-downs were done using Lipofectamine 3000 (Invitrogen) in antibiotic-free media ...
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bioRxiv - Molecular Biology 2019Quote: ... and the template plasmid was digested with Turbo DNase (Thermo Fisher #AM2239). Transcribed mRNAs were purified LiCl precipitation and were used for injection ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids containing PSHCP were transformed into BL21 (DE3) star cells (Invitrogen, C601003). PSHCP mutants were generated using Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... HaCaT cells were transfected with plasmid minigene and selected using G418 (Gibco). Expression and splicing of the minigene was assessed by RT-qPCR using primers presented in the reagent table.
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bioRxiv - Immunology 2021Quote: ... all transfections of plasmids were performed using Lipofectamine 2000 reagent (Life technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... cells were transfected with expression plasmids using Lipofectamine 3000 (Thermo Fisher; L3000015) following the manufacturer’s instructions and 3 μL of lipofectamine per μg of plasmid DNA ...
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bioRxiv - Biochemistry 2021Quote: ... E14 cells were transfected with plasmids carrying sgRNAs with Lipofectamine (Life Technologies). After two days ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid purification was performed from E.Coli One Shot TOP10/P3 (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were transformed in chemically competent bacteria strains Top10 (Thermo Fisher, C303003) and Stbl3 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Cloning of the plasmids was carried out using restriction enzymes (Thermo Fisher), PfuI polymerase (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected with pMSCV and pCl-Eco plasmids using Lipofectamine 3000 (Invitrogen) and cultured for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: Cells were first transfected with plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1200ng of Fibcd1 plasmid was mixed with 150µl Opti-Mem (Gibco #31985062), 700ng pGag/pol ...
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bioRxiv - Neuroscience 2019Quote: ... A LoxP-flanked EF1α-Puro-Tk cassette in a pENTR plasmid (Invitrogen) was inserted between the two homology arms ∼100bp upstream of the repeat region and in between the two Nsi1 sites ...
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bioRxiv - Cell Biology 2021Quote: ... with 0.2 μg plasmid (total) and 0.5 μl lipofectamine 2000 (Life technologies) and seeded in the centre of a 25-mm poly-L-lysine-coated coverslip ...
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bioRxiv - Microbiology 2020Quote: The plasmids were transiently transfected into the cells using Lipofectamine 3000 (Invitrogen) or Polyethylenimine ‘Max’ (Polysciences ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant plasmid was linearized using the Ncol restriction enzyme (ThermoFisher Scientific) and the MEGAscript® T7 Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and then cloned into the adenoviral plasmid pAd/CMV/V5-DEST (Invitrogen) using LR ClonaseTM II Enzyme Mix (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were transfected in a 1:2 ratio with Lipofectamine 2000 (ThermoFisher).
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bioRxiv - Physiology 2021Quote: ... template plasmid DNA was removed by FastDigest DpnI digestion (Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Microbiology 2019Quote: ... to make the plasmid pRG-PBG98-B-GFP11 (GeneArt, ThermoFisher Scientific, UK). DF-1 cells were co-transfected with this plasmid and pRG-PBG98-A to generate the recombinant virus PBG98-VP1-GFP11 ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with the indicated plasmids using Lipofectamine 2000 (Invitrogen) as per the manufacturer's instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was transfected in ExpiCHO-S cells (Gibco-Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was transfected in ExpiCHO-S cells (Gibco-Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... solution 1 was prepared by diluting plasmid into Opti-MEM (ThermoFisher Scientific). Solution 2 was prepared by diluting PEI (1 µg/µl ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection-grade plasmid DNA were isolated using a PureLink Maxiprep Kit (ThermoFisher).
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bioRxiv - Biophysics 2020Quote: ORF1 expression plasmids were transformed into Escherichia coli BL21STAR(DE3) cells (Invitrogen). One-liter cultures supplemented with 50 μg/mL kanamycin were inoculated with 20 mL of an over-night culture and grown at 37°C to an OD600 between 0.5 and 0.8 ...
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bioRxiv - Biophysics 2020Quote: ... and/or D3cpV plasmid DNA was transiently transfected using Lipofectamine 2000 (Invitrogen) mixed with OPTI-Mem (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid DNA was transfected using Lipofectamine LTX with plus reagent (ThermoFisher) or PEI (Polyetheleneimine ...