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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: Human HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Cat# 084564, Gibco, US) supplemented with fetal bovine serum (10% ...
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bioRxiv - Biochemistry 2024Quote: SH-SY5Y and HEK293/T cells were transfected with siRNA using Lipofectamine 3000 (Thermo Fisher) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Transfections were performed in HEK293 cells using Lipofectamine™ 3000 Transfection Reagent (L3000015, Thermo Fisher) and serum-free Opti-MEM I medium (31985 ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were tested for efficacy in HEK293 (ATCC) using a genomic cleavage kit (GeneArt, ThermoFisher). The best sgRNA was used for editing and had the sequence ...
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bioRxiv - Microbiology 2022Quote: ... and then stained with monoclonal anti-SARS-NP antibody conjugated to AlexaFluor488 (Invitrogen) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated-CoV proteins were captured on streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Spike protein (C9-tag) on the surface of pseudovirus was detected using a mouse anti-C9-tag monoclonal antibody 1D4 (Invitrogen, Cat. No. MA1-722), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Avi-tag for biotinylation and 6xHIS tag for Nickel purification using the HisPur™ Ni-NTA Spin Purification Kit (Thermo Fisher Scientific, Waltham, MA). The coated plates were washed 3x 300μl PBST and blocked for 1 hour in 1% BSA/PBS before adding a 1/3 titration of mAbs in assay diluent (0.5% BSA/PBS/0.05% Tween-20 ...
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bioRxiv - Developmental Biology 2020Quote: ... The target oligonucleotide was 3’ end labeled with biotin using a Biotin 3’ End DNA labeling kit (Thermo Fisher Scientific, Waltham, MA, USA, #89818). The oligonucleotides used as probes or competitors in gel shift assays were end-labeled at their 3’ ends with biotin ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were azide-labeled using Click-iT O-GlcNAc Enzymatic Labeling System and reacted with biotin alkyne using Click-IT Biotin Protein Analysis Detection Kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The enzyme concentration was calculated using absorbance measurements at 280 nm and the biotin concentration was determined using the Pierce Fluorescence Biotin Quantitation Kit (Thermo Scientific, catalog number: 46610). Biotinylation fraction was determined to be 60% ...
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bioRxiv - Plant Biology 2023Quote: ... The enzyme concentration was calculated using absorbance measurements at 280 nm and the biotin concentration was determined using the Pierce Fluorescence Biotin Quantitation Kit (Thermo Scientific, catalog number: 46610). The biotin:Cel7A ratio was determined to be 0.60 ...
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bioRxiv - Molecular Biology 2019Quote: ... PEG10 was immunoprecipitated with anti-PEG10 antibody pre-coupled to sheep anti-mouse Dynabeads (Thermo Fisher) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher, Waltham, MA, USA). Immunoprecipitation was performed using a KingFisher™ Flex Purification System (Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and a polyclonal anti-sheep antibody conjugated with Alexa Fluor 555 (A-31572; 1:1000, Invitrogen) were used ...
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bioRxiv - Immunology 2022Quote: ... thetaiotaomicron were cultured on trypticase soy-agar plates with 5% v/v sheep blood (ThermoFisher #B21239X) at 37°C while contained within an anaerobic chamber (BD GasPak #260683) ...
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bioRxiv - Cell Biology 2019Quote: ... centrifuged at 1500 rpm and the cells immediately incubated with Dynabead sheep anti-rat IgG (Invitrogen) coated with a rat anti-mouse ICAM-2 mAb (3C4 ...
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bioRxiv - Microbiology 2020Quote: Hemolytic activity was measured using Mueller-Hinton agar plates containing 5% defribinated sheep blood (Thermo Fisher) +/-L-161,982 at a final concentration of 50 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... sheep anti-digoxigenin (Life Technology PA185378) was used with Alexa conjugate secondaries (1:400; Thermo Fisher). For alkaline phosphatase (AP ...
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bioRxiv - Microbiology 2023Quote: ... Presumptive macrococci colonies were streaked onto blood agar supplemented with 5% sheep blood (Oxoid, ThermoFisher, Johannesburg), incubated for 24 hours at 37°C ...
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bioRxiv - Genomics 2022Quote: ... washing with cold PBE and binding the DP cells to Dynabeads-sheep anti-rat IgG (Invitrogen). The supernatant was centrifuged to harvest the cells ...
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bioRxiv - Pathology 2023Quote: ... 1:200 (for fibronectin) or donkey anti-sheep IgG Alexa 488 (A-11015, Thermo Fisher Scientific) at 1:200 and donkey anti-rabbit IgG Alexa 647 (A10040 ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 350 donkey anti-sheep IgG (H+L) (1:250, A-21097, Thermo Fisher Scientific), Alexa Fluor 488 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... This solution was mixed with 0.4 mL of Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen) previously conjugated with 25 ug of S9.6 antibody (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Cell Biology 2020Quote: ... and/or V5 tags were constructed with pcDNA6 vector (Invitrogen). Recombinant human Lsm12-expression plasmids were obtained either commercially (pCMV-Lsm12-Myc-FLAG from OriGene ...
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bioRxiv - Genetics 2021Quote: ... triacylglycerol or TAG (Pointe Scientific Triglycerides Liquid Reagents; Fisher Scientific); non-esterified fatty acids or NEFAs (HR series NEFA-HR[2] Reagents ...
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bioRxiv - Immunology 2021Quote: ... anti-VSVg tag-158 Gd (rabbit pAb, Thermo Fisher Scientific), anti-E tag-154Sm (clone 10B11 ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled with TMT10plex Mass Tag Labelling kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... labeled with 16-plex tandem mass tag reagents (Thermo Scientific) in 100 mM triethylammonium bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with primary antibodies against V5 tag (R960- 25, ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His tag Antibody (MA1-125 21315) was from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-DYKDDDDK epitope (FLAG) tag (Thermo Scientific Pierce, MA1-91878), Anti-HA (Thermo Scientific Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... coli expression vector pDEST15 with GST tag Gateway Vector (Invitrogen). MYC-LRRK2-WT was a gift from Dr ...
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bioRxiv - Cell Biology 2020Quote: ... anti-S-tag mouse monoclonal antibody (MA1-981; Thermo Fisher), rabbit polyclonal anti mNeonGreen Tag (53061S ...
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bioRxiv - Systems Biology 2020Quote: ... We dissolved tandem mass tag (TMT) 10plex reagents (Thermo Scientific) in anhydrous acetonitrile (0.8 mg/82 μl) ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary C-MYC tag antibody (PA1-981, Thermo Fisher Scientific) and N-MYC Polyclonal antibody (PA5-17403 ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... labelled with Tandem Mass Tag (TMT) 10-plex (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... An anti-His-tag antibody coupled to HRP (Thermo Scientific) was used for enzymatic detection ...
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bioRxiv - Microbiology 2021Quote: ... HRP V5-tag monoclonal antibody (1:1000, Invitrogen, # R961-25) was used to detect protein.
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bioRxiv - Systems Biology 2022Quote: ... mouse anti-HA-tag (ThermoFisher, #26183, 1:500 for IF), mouse anti-Rab5A (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG epitope tag (Mouse mAb; ThermoFisher Scientific [FG4R], MA1-91878), GFP (Rabbit pAb ...
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bioRxiv - Cell Biology 2020Quote: ... Tandem mass tag (TMT) isobaric reagents (Thermo Fisher Scientific, 90406) were dissolved in anhydrous ACN to a final concentration of 20 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-V5 tag (Invitrogen, R960-25, 1:1:500), and mouse anti-αTUBULIN (B512 ...
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bioRxiv - Microbiology 2021Quote: ... and V5 tag monoclonal antibody (1:100, Invitrogen, # R960-25) for checking C1QL3 binding ...
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bioRxiv - Cancer Biology 2020Quote: ... 2μg anti-V5-Tag (ThermoFisher Scientific; catalog number: 37-7500), 2μg anti-IRF3 (BioLegend ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-GFP tag polyclonal (1:600, Thermo Fisher Scientific) and mouse anti-Repo (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-HA.11 antibody was purchased from Biolegend and antibodies against V5 and GST tags were from ThermoFisher.