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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 40 μl of streptavidin magnetic beads were incubated with 4 μg of anti-CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, #13-1719-82, Thermo Fisher) for three hours on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Psy2-13Myc was immunoprecipitated using anti-Myc antibody (MC045, Nacalai Tesque) These were bound to Dynabeads Protein G (Thermo Fisher Scientific) from meiotic yeast cell lysate prepared using a Multi-Beads Shocker (YASUIKIKAI ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatants were incubated with anti-GFP-antibody (Cat# N86/38, clone N86/38, UC Davis/NIH NeuroMab) pre-bound to Protein G Dynabeads (Cat# 10004D, Life Technologies) on a shaking rotator for 3h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1D4 tagged MEGF8 was captured by a 1D4 antibody (The University of British Columbia) covalently conjugated to Protein A Dynabeads (Thermo Fisher Scientific, Invitrogen). Immunoprecipitates were washed once with IP Wash Buffer A (50 mM Tris at pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The positive control was addition of 10 μg/mL of SARS-CoV-2 spike protein antibody (pAb, T01KHuRb) (ThermoFisher, MA, USA) at the time of infection.
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bioRxiv - Microbiology 2021Quote: ... Labelled proteins were stained for 1 hour at room temperature using Alexa Fluor 488/594-conjugated goat antibodies (1:2000, Life Technologies). Nuclei were stained using the intercalating DNA dye DAPI at 5 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... using 100 μg custom monoclonal anti-GFP antibodies (50 μg of clone 19C8 with 50 μg of clone 19F7) bound to biotinylated Protein L (Pierce, Thermo Fisher) coated streptavidin-coated magnetic beads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The chromatin + antibody were subsequently bound for 20 minutes at room temperature to magnetic beads (Dynabeads protein G, Life Technologies #10004D) that were pre-incubated with BSA ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Neuroscience 2020Quote: MARK4 expressed in HEK293 cells was immunoprecipitated from the cell lysate with monoclonal anti-Myc antibody (4A6) and Dynabeads protein G (Thermo Fisher). Its kinase activity was measured using human 2N4R tau ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... PI3K was then immunoprecipitated with 5 μl of antibody (anti-p85α, MilliporeSigma, Burlington, MA, USA) and 60 μl of Pierce Protein A/G magnetic beads (Thermo Scientific). PI3K activity in the immunoprecipitates was then assayed by PI3K ELISA according to the manufacturer's instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein detection was facilitated using species-specific antibodies conjugated to horseradish peroxidase and Pierce® Peroxidase IHC Detection Kit (Thermo Scientific) or species-specific secondary antibodies with IRDye Fluorescent Dyes (IRDye 800CW goat anti-rabbit IgG ...
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bioRxiv - Plant Biology 2020Quote: ... Protein was detected using chemiluminescence with 1:3000 Anti-Guinea pig secondary HRP-coupled antibody (Thermo Fisher Sci Cat No. A18769). Membrane was stripped and tubulin was detected as a loading control by incubation with 1:25,000 Mouse monoclonal anti-TUB DM1A (Abcam) ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... Sheared chromatin was cleared and incubated overnight at 4 degrees with primary antibodies that were pre-incubated with protein G magnetic DynaBeads (Thermo Fisher). Primary antibodies used included anti-H3K27Ac (9ug/sample ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were incubated with anti-FLAG or anti-Myc antibodies in the presence of protein G or A magnetic beads (Thermofisher Scientific) for 2 hours at 4°C while slowly rotating ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were purified from the cell medium 5 days after transfection by affinity chromatography using Protein A columns (Thermo Fisher Scientific). Soluble CD4-Ig (sCD4-Ig ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 5% FBS and 0.5% Triton X-100) for 30 minutes and washed before incubation with an anti-SARS-CoV-2 spike protein antibody (Life Technologies). Nuclei and F-actin were stained using DAPI and Phalloidin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
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bioRxiv - Genetics 2020Quote: ... 4 mg total extract, 10 mg anti-ollas antibody (A01658-40, GeneScript) and 30 ml Protein A/G magnetic beads (Thermo Scientific) were used per technical replicate ...
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bioRxiv - Neuroscience 2023Quote: ... For anti-Syp immunoisolation, anti-Syp (101 011, SySy) antibody was pre-incubated with Pierce protein G magnetic beads (88848, Thermo Scientific) in 0.1% BSA PBS at 4 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... or 4 μl of rabbit polyclonal anti-GAF [101] antibodies per sample were immobilized on Protein G Dynabeads (Thermo Fisher Scientific), incubated with lysates for 90 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... The axonemal proteins were incubated overnight with anti-HA magnetic beads with bound anti-HA antibodies (Thermo Fisher Scientific, Waltham, MA) at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were performed overnight at 4°C on 40μg of chromatin with antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific). The antibodies used are listed in Table S10 ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... was used as a secondary antibody and the resulting proteins of interest were visualized with SuperSignal West chemiluminescent substrate (Thermo Fisher). Ponceau S or Amido Black staining was performed to verify equal loading.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates from cells were cleared by centrifugation and were incubated with either HA (Benthyl) or COPB2 (Cusabio) antibodies-Dynabeads Protein G (Thermo Fisher) overnight at 4°C ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Membranes were then incubated with the corresponding counter-antibody and the proteins were revealed by enhanced chemiluminescence detection (SuperSignal West Femto System, Thermo Scientific). For information about the antibodies used please see Supplementary Table S3 ...
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bioRxiv - Cancer Biology 2022Quote: Purified anti-HLA-I clone W6/32 (ATCC® HB95) antibodies were cross-linked to protein-A Sepharose 4B conjugate beads (Invitrogen) with dimethyl pimelimidate dihydrochloride (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was incubated overnight at 4 °C with the anti-Kv2.1 rabbit polyclonal antibody KC (Trimmer, 1991) and then incubated with 100 μL of magnetic protein G beads (ThermoFisher Cat# 10004D) for 1 h at 4 °C on a tube rotator ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by using horse radish peroxidase (HRP)-conjugated secondary antibodies and SuperSignal West Dura Extended Duration Substrate (Thermo Scientific). Images were taken with a ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with relevant secondary antibodies for 2 h at room temperature and immunoreactive proteins were revealed using ECL substrate (Fisher Scientific). Light signal was digitalised using the ImageQuant LAS4000 Imager digital system (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Western blotting and immunodetection of HA-tagged proteins with anti-HA and horse radish peroxidase-coupled secondary antibodies using an iBright™ FL1500 (ThermoFisher). The remaining 25 µl bead suspension was used as input for on-bead digestion (Klaus et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 37.5 µg affinity purified anti-CDCA7 or anti-HELLS antibodies was coupled to 150 µl Protein A Dynabeads (Thermo Fisher Scientific) and used to deplete 100 µl extract at 4 °C for 45 min ...
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bioRxiv - Molecular Biology 2023Quote: ... or 4 μl of IgG control rabbit antibody at 1 mg/ml (SigmaAldrich I5006) were combined to 50 μl of protein A magnetic beads (Invitrogen 10001D) and added to sonicated chromatin (from 200 to 700 bp ...
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bioRxiv - Biophysics 2023Quote: ... Protein bands were transferred to a nitrocellulose membrane and immunoblots were probed with anti-HA primary antibody (Thermo Fisher 26183,1:10000) and anti-mouse secondary antibody (Abcam ab205719 ...
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bioRxiv - Cell Biology 2023Quote: ... DYRK1A-dependent phosphorylation of Thr212 of MAPT/Tau protein was evaluated by Western blotting with anti-phospho Tau (Thr212) antibody (Invitrogen, #44740G).
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bioRxiv - Plant Biology 2024Quote: ... or anti-pTAC3 antibody (PhytoAB, San Jose, CA, USA; catalog number PHY0391A) and with 60 μL Protein A Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... sonicated chromatin was incubated overnight at 4°C with antibodies that had first been coupled to Protein G beads (Thermo Fisher). After incubation ...
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bioRxiv - Developmental Biology 2022Quote: ... was added to the sonicated chromatin and incubated with the antibody-bead complex (Pierce Protein A/G Magnetic Beads, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were mixed with either 0.8 μg/mL of rat anti HA antibody and 30 μL of pre-washed dynabeads protein G (Thermo Fisher; 10003D) or 40ul of pre-washed anti-Flag M2 affinity gel (Millipore Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Invitrogen, 10001D). The following antibodies was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4 °C with mouse anti-ee antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific( ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the Cetuximab antibody was calculated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). The loading ratios and conjugation efficiencies of the two dyes were subsequently calculated using the obtained concentrations of the dyes and protein (antibody) ...