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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... A small-scale plasmid preparation was performed on the culture to isolate the plasmid using the GeneJET Plasmid Miniprep Kit (Thermo Fisher). This plasmid was digested using XbaI and BstXI (5’ tag ...
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bioRxiv - Neuroscience 2024Quote: ... The regions of interest were micro dissected under a dissecting microscope and transferred to a small Petri dish containing 3 ml of Papain mix consisted of Hibernate mix (Hibernate A medium (Invitrogen A1247501), 1xGlutamax (Gibco 35050-038) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Calu-3 cells and A549 (a human non-small cell lung carcinoma cell line) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, 11965118, Thermo Fisher Scientific) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Genomics 2024Quote: The tissue was minced into small pieces using sterilized scissors and placed in 0.05% trypsin-EDTA solution (Catalog No. 25300054, Thermo Fisher Scientific), followed by gentle dispersion with a pipette ...
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bioRxiv - Evolutionary Biology 2024Quote: ... they were transferred to a small petri dish filled with Ca/Mg-free ASW containing 40U/ml ofSUPERase•In™ RNase Inhibitor (Invitrogen), where they were allowed to incubate for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The devices were then cut in small squares (approximately 1×1 cm2) and attached to plasma-cleaned coverslips (#1.5, 22×22 mm, Thermo Fisher Scientific), and incubated at 85 °C for 1 hour.
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bioRxiv - Cell Biology 2020Quote: ... The extracts were incubated at 70 °C for 45 min and used for protein analysis or RNA isolation using Trizol LS (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: 100 nM CoV firefly luciferase fusion RNA was added to 12 µL of HeLa lysate reaction mix from an in vitro protein expression kit (Thermo Scientific, 88882), along with 0.5 µL of rocaglamide/RocA solution (Med Chem Express ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: 1 μg of IRE1-KR recombinant protein was crosslinked in 25 μl final volume of RNA cleavage buffer containing 1 μl of disuccinimidyl suberate (DSS, Thermo Fisher Scientific) crosslinker at 6.25 mM (final concentration is 250 μM ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from blood samples according to the Qiagane AllPrep DNA/RNA/Protein isolation kit and its quantity was measured using a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Cortical neurons were plated at a density of ∼500 cells/mm2 (for RNA or protein isolation) or ∼250 cells/mm2 (for immunocytochemistry) on tissue culture plates or coverslips (Fisher Scientific, NC0672873) coated with 0.1mg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA that was bound to target protein and RNA from input samples were purified from the beads with TRI-ReagentTM solution (Thermo Fisher Scientific), precipitated by adding 1 volume (∼300 µl ...
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bioRxiv - Microbiology 2022Quote: ... or with 200 μL of Exosome Resuspension Buffer (1-2.5% Triton X-100) from the Total Exosome RNA and Protein Isolation Kit (Invitrogen, Thermo-Fisher Scientific) to lyse the EVs for proteomic analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 6 ul 30 μM guide RNA (AAVS1 and BRD1 from IDT) and 2 µl 30 μM TrueCut Cas9 Protein v2 (catalog number A36499; Thermo Fisher Scientific) were gently mixed by pipetting up and down in a PCR strip and incubated at room temperature for 20 min.
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bioRxiv - Immunology 2021Quote: ... The neutrophils were identified as S100A8-FITC (CF-145) and TNFAIP6-AF594 (polyclonal) (both ThermoFisher Waltham, MA) double positive cells ...
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bioRxiv - Cell Biology 2021Quote: ... by injecting in the duct of Cuvier a solution containing FITC-dextran (70 kDa) (Life Technologies, Inc.) at 1 mg/ml concentration ...
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bioRxiv - Physiology 2022Quote: ... Mitochondrial mass (FITC/PE channel) was measured by fluorescence levels upon staining with MitoTracker Green (Invitrogen, M7514) at 100 nM for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were stained with anti-Toxo-fluorescein isothiocyanate (anti-Toxo-FITC) (Thermo Fisher, catalog no. PA1-7253) antibody by incubating overnight at 4°C in 3% BSA in 1X PBS at 1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in 1% Triton-X in PBS with FITC rabbit anti-mouse IgG1 (1:400, Invitrogen A21121) and Alexa fluoro-568 conjugated a-bungarotoxin (1:500 ...
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bioRxiv - Microbiology 2021Quote: Porphyromonas cell-free supernatants were tested for general protease activity using fluorescein-labelled casein (FITC-Casein, ThermoFisher) as a substrate ...
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bioRxiv - Neuroscience 2024Quote: ... then in 1% Triton-X in PBS with FITC rabbit anti-mouse IgG1 (1:400, Invitrogen A21121) and Alexa fluoro-568 conjugated α-bungarotoxin (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... The remainder was stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA (Fraction V ...
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bioRxiv - Biochemistry 2023Quote: Dead Cell Apoptosis Kit with Annexin V FITC and PI for flow cytometry (#V13242, Thermo Fisher Scientific) was used to study the effects of intermittent high glucose exposure on endothelial apoptosis ...
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bioRxiv - Cell Biology 2024Quote: ... differently sized FITC-dextrans (3.3 kDa, D3305; 40 kDa, D1845; 70 kDa D1822, all purchased from ThermoFisher) were applied at 10 µg/mL and immediately imaged using Opera Phenix High Content Imaging System (PerkinElmer ...
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bioRxiv - Biophysics 2021Quote: ... DNA quality was determined by 260nm/230nm and 260nm/280nm ratios on a DS-11+ spectrophotometer (DeNovix) and concentration was determined using the Qubit 3 (Thermo Scientific). Pooled samples were sent to GeneWiz where they were analyzed by TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... The enriched libraries were diluted to a final concentration of 11 pM and subjected to sequencing from a single end in a Ion Proton Sequencer (Life Technologies). FASTQ files were mapped to the UCSC mouse reference genome (build mm9 ...
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bioRxiv - Cell Biology 2021Quote: ... human female adenocarcinoma of the mammary gland) were maintained in Dulbecco’s Modified Eagle Medium High Glucose (ThermoFisher Scientific, #11-965-118), 10% foetal bovine serum (Bovogen ...
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bioRxiv - Immunology 2022Quote: VeroE6-eGFP were cloned and validated in-house as previously described.11 Cells were cultured and maintained in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, USA) supplemented with 10% (vol/vol ...
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bioRxiv - Biophysics 2022Quote: ... in 20 mM CAPS pH 11 was added and mixed thoroughly by pipetting to initiate phase separation and the plate was sealed (Thermo Scientific). ThT fluorescence was read at 10-minute intervals for 24 hours at 25°C using a Tecan Infinite M200 plate reader (Tecan Trading AG) ...
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bioRxiv - Biophysics 2022Quote: ... and SN25 FL (1–206, R59C) or truncation mutant (11–206, R59C) were labeled with 5×molar excess Tetramethylrhodamine-5-maleimide (TMR) (Molecular Probes) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Systems Biology 2021Quote: ... Aliquots of 15 μg of total protein extract from each sample (3 strains x 4 conditions x 3 replicates) and the three bulks were separated on onedimensional SDS-PAGE short-migration gels (11×1 cm lanes, Invitrogen, NP321BOX). Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... were prepared for mass spectrometry using the Preomics iST-NHS (Preomics, catalogue # P.O.00026) and TMT 11-plex labelling (ThermoFisher, catalogue # A37725) kits according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... the gypsy backbone used in previous publication (11) was amplified and cloned into the NotI/XbaI sites of pAc5.1/V5-His version B (V411020, Thermo Fisher Scientific). To synthesize the final pAc-gypsy-H2B-mCherry vector ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Immunology 2023Quote: SVG-A and 293T cells were infected with JCPyV M1-SVEΔ for 9-11 days and stained first with the LIVE/DEAD® Fixable Aqua Dead Cell Stain Kit (Invitrogen), then cells were split and stained in parallel with one of the following antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested for up to 11 days and the cell number recorded using a Countess IIR automated cell counter (Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with either scrambled (non-targeting #1) siRNA or Septin9 siRNA (J-048947-11-0050 ONTARGETplus) with Lipofectamine-iMAX (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides from each individual case and the GIS pooled standard or bridging sample were labeled using the TMT 11-plex kit (ThermoFisher 90406). For each batch ...
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bioRxiv - Microbiology 2022Quote: ... 1mM DTT, 300 mM KCl, 20µM biotin-11-ATP, -UTP, -GTP, -CTP, 0.4 U/ml RNase inhibitor [RNaseOUT ThermoFisher] and 1% Sarkosyl) was added to thawed nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... non-coated 35 mm dish and single fibers were gently released with serial trituration into 2 mL of DMEM (catalog number 11-965-092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL was loaded onto an IonPac AS-11 HC strong anion-exchange analytical column (2 mm × 250 mm, 4 µm particle diameter, Thermo Scientific) with AG-11 HC guard column (2 mm × 50 mm ...
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bioRxiv - Genomics 2024Quote: ... DNA quantity was checked with fluorometry on the DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and sizes were examined on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentration of eluates was assessed using a DS-11 FX+ Spectrophotometer (Denovix) using a Qubit™ dsDNA High Sensitivity Assay Kit (Invitrogen). 200 ng of each individually extracted sample was used to pool by cage (4 samples sent for sequencing).
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bioRxiv - Molecular Biology 2023Quote: ... DNA quantity was tested with fluorometry on a DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (Thermo Fisher). Sizes were analyzed on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Systems Biology 2023Quote: We conducted mass spectrometry analysis of 120 CSF samples using 13 batches of 11-plex tandem mass tag (TMT, Thermo Scientific) experiments ...