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Citations for Thermo Fisher :
2701 - 2750 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% Fetal Calf Serum (FCS) and 1% Antibiotic Antimycotic (Gibco, Thermofisher, Netherlands) was added to each swab sample ...
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bioRxiv - Immunology 2022Quote: ... and pellet resuspended in 200uls MACS + Fc-block (ThermoFisher, catalog no. 14-9161-73). Cells were stained for surface antigens using fluorescence-conjugated monoclonal Abs specific to human CD45 ...
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bioRxiv - Immunology 2022Quote: ... Fcs and IgGs were synthesized and cloned by GeneArt (Thermo Fisher Scientific, Waltham, MA) in the pcDNA3.4 expression vector and transiently expressed in HEK 293F cells ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... with glutamax supplemented with 10% FCS and 10 µg/ml Blasticidin (Invitrogen, R210-01). HCT 116 _CRISPR-Cas9 cells were cultured like the parental cell line supplemented with 2 µg/ml Puromycin ...
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bioRxiv - Cell Biology 2019Quote: ... and 10% FCS in culture dishes (100 mm with vents; Fisher Scientific UK Ltd) at 37°C in a humidified incubator at 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... containing 10% FCS and 100 units/ml penicillin-streptomycin solution (Pen/Strep, Gibco, Belgium). Virus culture and cytopathic effect-based virus neutralization assays (CPENT ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowaste) and 1% penicillin/streptomycin (Life Technologies).
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bioRxiv - Synthetic Biology 2020Quote: ... Absorbance at 405 nm was recorded with Multiskan FC microplate photometer (Thermo Fisher Scientific). All shaking steps were conducted in Titramax 1000 (Heidolph Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... TβRII-Fc (124 kDa) and murine mAb 1D11 (150 kDa) (Thermo Fisher, Waltham, MA) were each captured on individual flow cells to 100-140 RU ...
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bioRxiv - Immunology 2020Quote: ... 2 mM L-Glutamine and 50 µm 2-Mercaptoethanol) with 10% FCS (all Gibco). Colon and caecal tissue were digested in an enzyme cocktail of 0.45 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Physiology 2019Quote: ... The ELISA was read using a filter-based accuSkan FC micro photometer (Fisher Scientific). The limits of sensitivity were <0.188 ng/ml and < 0.375 ng/ml for MUC5AC and MUC5B ...
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bioRxiv - Microbiology 2021Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was blocked by transferring the halves to a dish containing FCS (Gibco, USA) and the epidermis peeled off the dermis ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked prior to staining using anti-CD16/32 (93, ThermoFisher Scientific). Where >1 brilliant violet or brilliant UV dye was used concurrently ...
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bioRxiv - Immunology 2022Quote: ... followed by Fc-receptor blockade with anti-CD16/CD32 (Thermo Fisher #14-0161-85), and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2023Quote: Cells were then frozen in fetal calf serum (FCS) (ThermoFisher Scientific, Cat. No. 10500064) 10% DMSO (v/v ...
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bioRxiv - Immunology 2022Quote: ... Optical density (OD) was measured using a Multiskan™ FC (Thermo Fisher-Scientific UK) plate reader at 405 nm and S1-specific IgG titers were interpolated from the IgG standard curve using 4PL regression curve-fitting on GraphPad Prism 9.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Medium was removed and cells were transferred in FCS-free essential medium OptiMEM (Gibco) 2 h before transfection ...
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bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen; 14-0161-85). The cells were then incubated for 30 minutes with the surface antibodies and Live/Dead Ghost Dye Violet 510 (Tonbow Biosciences ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to the manufacturer’s instructions using a Multiskan™ FC microplate photometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Gibco Cell Culture, 10270-106), 0.5% (v/v ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were incubated with Fc Block CD16/32 (Invitrogen, 14-0161-85). The cells were then incubated with a 1:200 dilution of antibody for surface stains and A Live/Dead Ghost Dye Violet 510 (Tonbo Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved at −150°C in 40% heat-inactivated fetal bovine serum (FCS, Gibco), 50% RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-CD16/CD32 (diluted 1:50, Thermo Fisher Scientific) and cells were stained with cell surface antigens ...
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bioRxiv - Cell Biology 2023Quote: ... After Fc receptor blockage using an antibody for CD16/32 (14-0161-85, Invitrogen), stainings were performed in FACS buffer (1 % BSA ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FCS (Invitrogen, 10270-106, batch number 41F8126K), 2,000 units ml−1 LIF (Millipore ...
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bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
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bioRxiv - Bioengineering 2024Quote: ... Siglec-Fc constructs were expressed according to the Expi293F manufacturer protocol (Thermo Fisher Scientific) and purified by manual gravity column (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS, Biowaste) and 1% penicillin/streptomycin (Gibco, 15140).
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bioRxiv - Genetics 2024Quote: ... Fc glycans were released and labelled using the GlycanAssure ATPS kit (Thermo Fisher Scientific) then separated using 3500xL Genetic Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... A region spanning 2163 bps upstream from the transcription start site was PCR amplified using genomic DNA isolated from HEK293 cells with Platinum SuperFi II DNA polymerase (Thermo Fisher Scientific). The PCR product was cloned into pGL3 basic vector following conventional protocols ...
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bioRxiv - Cell Biology 2020Quote: Samples prepared following immunoprecipitation from HEK293 MceC3XFLAG cell line were analysed by LC-MS on an Orbitrap Elite™ mass spectrometer (ThermoFisher Scientific) coupled to a Dionex Ultimate 3000 Ultra-Performance Liquid Chromatography (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Phoenix cells were transfected with the pBabe plasmid DNA encoding for the protein of interest using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2019Quote: ... Transfection of Flp-In T-Rex HEK293 cells was carried out according to Flp-In™ T-REx™ Core Kit (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were transfected with pcDNA3.1-GAS6-MycHis linearized with PvuI restriction enzyme using Lipofectamine® 2000 Transfection Reagent (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The P2 baculovirus produced in Sf9 cells was added to HEK293 GnTI- cells (mycoplasma test negative, ATCC #CRL-3022) and grown in suspension in FreeStyle medium (GIBCO-Life Technologies) supplemented with 2% FBS at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...