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Citations for Thermo Fisher :
2651 - 2700 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... stimulated with CD3/CD28 T-Activator and mouse IL-2 (Gibco), and cocultured with F4/80+ macrophages at 2:1 (T cells:macrophages ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific, Rockford, IL, USA) were used for subcellular fractionation of BC cells ...
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bioRxiv - Neuroscience 2024Quote: ... were coated with IL-6 capture antibody (ThermoFisher Scientific, MP5-20F3) and left overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... comprising M-PER Mammalian Extraction Buffer (Thermo Scientific, Rockford, IL, USA), Halt protease inhibitor cocktail (Complete Mini EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2024Quote: ... and colleagues [19] with 1X Glutamax (Thermo Scientific, Rockford, IL, USA), 10mM Hepes (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: Cell lysates were collected in RIPA buffer (Thermo Fisher, Rockford, IL) containing a protease and phosphatase inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... containing a protease and phosphatase inhibitor cocktail (Thermo Fisher, Rockford, IL). Samples were mixed with 4X lamelli buffer (Bio-rad ...
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bioRxiv - Microbiology 2024Quote: ... immersed in Super Signal West Femto (Thermo Fisher Scientific, Rockford, IL) and visualized using an LAS 4000 Mini imaging system (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-NLRP3 (Thermo Scientific, Rockford, IL, USA; 1:300) were incubated over-night ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dye were used in this study: anti-rat E-cadherin (Invitrogen, 13-1900, 1:300), anti-rabbit cleaved caspase-3 (Cell signaling ...
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bioRxiv - Immunology 2021Quote: ... 13-0443-82) and 1:200 VE-cadherin-Alexa Fluor 647(rat anti-mouse, clone 11D4.1, Thermo Fisher, BDB562242) in 1.25% BSA in PBS for 24 hours ...
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bioRxiv - Neuroscience 2019Quote: For live imaging experiments primary hippocampal cultures were transfected at 10-13 DIV using Lipofectamin 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions and imaged 48h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... a homolog of the vaccinia virus H3L gene) [13] using the Path-ID™ qPCR Master Mix (Thermofisher Scientific). Nucleic acid from capripoxvirus positive samples was further analysed by a qPCR targeting LSDV011 designed to differentiate the three capripoxvirus species [14] and a qPCR targeting LSDV008 designed to differentiate LSDV wildtype strains from Neethling vaccine strains [15] ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
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bioRxiv - Plant Biology 2020Quote: ... All conductivity measurements were performed with an Accumet 200 conductivity meter (Fisher Scientific, probe: catalog number 13-620-101). Initial readings were collected from samples incubated at 0°C for 30 min in a pre-cooled chiller (initial measurement) ...
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bioRxiv - Cell Biology 2020Quote: ... referred to herein as INS-1 832/13) were cultured in RPMI 1640 medium with 11.1 mM D-glucose (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2021Quote: ... The neuronal cultures were then incubated for 13-20 DIV in Neurobasal™-A supplemented with B-27 (Invitrogen), 50 IU/ml penicillin and 50 μg/ml streptomycin at 37°C before experimental procedures ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific, 43-688-13) according to manufacturer’s instructions ...
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Biodiversity and Antimicrobial Potential of Bacterial Endophytes from Halophyte Salicornia brachiatabioRxiv - Microbiology 2020Quote: ... Fractions 11 and 13 containing bioactive compounds were further chromatographed on semi-preparative HPLC (Dionex Ultimate 3000, Thermo Scientific) using gradient mixtures of acetonitrile–water (4:1 to 9:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... was used to extract RNA and fragmented for 13 mins in 70°C using RNA Fragmentation Reagents (Life Technologies) and later purified by running through RNase-free P-30 column (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... first dimensional lanes are cut from the gel and incubated in 1% SDS with 1% beta-mercaptoethanol for 1 hour before the gel slice was loaded into an NuPAGE 4-13% 1.0mmx2D gel (Invitrogen). Gels were run at 200V for 50 minutes at room temperature in MOPS ...
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bioRxiv - Physiology 2021Quote: The INS-1 832/13 cells (gift from C. Newgard PhD, Duke University) were cultured in RPMI-1640 (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified DNA extracts were concentrated from approximately 35 μL to ~13 μL using vacuum centrifugation (SpeedVac, Thermo Fisher Scientific). Quantitation was performed on all donor reference samples purified with Promega’s Plexor ®
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bioRxiv - Immunology 2020Quote: 1×105 A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA(D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior a second round of reverse transfection ...
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bioRxiv - Immunology 2022Quote: ... and cloned using SacI and ApaI in the pCR3 SARS-CoV-2-SΔ19 expression plasmid (13) using Gibson Assembly (ThermoFisher). All constructs were verified by Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) with 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
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bioRxiv - Neuroscience 2022Quote: ... The neuronal cultures were then incubated for 13-20 DIV in Neurobasal™-A supplemented with B-27 (Invitrogen), 50 IU/ml penicillin and 50 μg/ml streptomycin at 37°C before experimental procedures ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was converted into cDNA using the High Capacity cDNA Reverse Transcriptase kit (Thermo Fisher Scientific #43-688-13) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was reverse-transcribed to cDNA by High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, 43-688-13). A SYBR Green-based master mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and water) and polytetrafluoroethylene (PTFE) syringe filters (0.45 μm, 30 mm; 0.22 μm, 13 mm) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2024Quote: Retinas were cultured on 13 mm/0.2 micron floating filters (Cytiva, 10417001) on DMEM/F-12 media (Gibco, 11320082) with 10% Fetal Bovine Serum (Thermofisher ...
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bioRxiv - Biophysics 2024Quote: ... The experimental and control devices were then placed inside an environmental chamber (Thermo Scientific, Catalog no. 13-067-066) and incubated at 30 ◦C and 50 % humidity ...
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bioRxiv - Bioengineering 2024Quote: ... and RNA quality/yield were then measured using a NanoDrop™ OneC spectrophotometer (Thermo Fisher Cat#13-400-519) prior to cDNA synthesis ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) and 1% penicillin/streptomycin (Gibco). BT-549 and HCC1806 were cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Immunology 2021Quote: ... The signals were read at 450 nm using accuSkan FC microplate photometer (Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... SDM79 and SDM80 were supplemented with 7.5 mg/l hemin and 10% FCS (Invitrogen), and cells were grown at 27°C continuously in these media ...
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bioRxiv - Immunology 2022Quote: ... and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10% FCS and 100 units/mL Penicilium and 100 μg/mL Streptomycin (Gibco). To generate overexpressing cells ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA constructs for Fc-NKG2D molecules were expressed in Expi293TM cells (Thermo Fisher Scientific) and dimeric secreted protein was purified by Protein A affinity chromatography (PierceTM #20334 ...
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bioRxiv - Physiology 2020Quote: ... The ELISA was read using a filter-based accuSkan FC micro photometer (Fisher Scientific). The limits of sensitivity were > 0.188 ng/ml and > 0.375 ng/ml for MUC5AC and MUC5B ...
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bioRxiv - Bioengineering 2021Quote: ... cells were blocked with an anti-Fc Receptor polyclonal antibody (Invitrogen 14-9161-73) for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... point FCS measurements with Alexa Fluor® 488 (Thermo Fisher Scientific, Waltham, MA, USA) dissolved in water at 20 nM were performed at the same laser power ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 2% v/v heat-inactivated FCS and sodium bicarbonate (Gibco 25080-060). Diluted compounds were then mixed with EGFP-expressing Vero E6 cells at 25,000 cells/well in 96-well plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% of Penicillin-Streptomycin mixture (Gibco) at 37°C 5% CO2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... followed by incubation with HRP-conjugated goat anti-Fc antibody (Invitrogen, catalog number A18817). Stained cells were visualized using KPL TrueBlue peroxidase (SeraCare ...
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bioRxiv - Immunology 2020Quote: ... Washing medium consisted of 2% FCS and 2% P/S in RPMI 1640 (Gibco). FACS buffer contained 2% FCS and 2mM EDTA in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance at 450 nm was read using an accuSkan FC microplate reader (Fisher Scientific) with SkanIt software (Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed once with RPMI medium (Gibco, w/o glucose, FCS and PSG) and incubated in the same medium for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% Fetal Calf Serum (FCS) and 1% Antibiotic Antimycotic (Gibco, Thermofisher, Netherlands) was added to each swab sample ...