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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: HEK293 and A549 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) containing 4 mM glutamine and 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Transient transfections were performed with either lipofectamine 2000 reagent from Invitrogen (Hek293 cells, MDCK) or with FuGENE 6 (ProMega ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells (CRL-1573, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (#11965126, Gibco) containing 10% fetal bovine serum (12306C ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells (ATCC CRL-1573) were grown in Dulbecco modified Eagle medium (DMEM, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
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bioRxiv - Molecular Biology 2023Quote: HEK293T or HEK293-JumpIN cells were cultured with Gibco DMEM with Glutamax (Life Technologies) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transfected into HEK293-T or LNCaP cells using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples generated from HEK293 cells were analyzed with an LTQ-Orbitrap Velos (Thermo Fisher) while samples from HeLa cells with a Q Exactive (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: 5×105 HEK293 cells were plated on a 35 mm cell culture dish (Nunc) one day before transfection ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells and A549 cells were cultured in DMEM media (Thermo Fisher #11965-092) containing 1% Pen/Strep and 10% FBS (VWR International ...
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bioRxiv - Biochemistry 2023Quote: HEK293 and C6 glioblastoma cells were cultured in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal calf serum (HI-FCS ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... fixed HEK293 RyR2D4365-GFP cells were incubated with MA3-916 RyR2 monoclonal antibody (Invitrogen) diluted 1:100 at 4°C overnight in the same solution as above ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (ATCC) and were all grown in 1x DMEM based media (ThermoFisher Scientific) with 1% Penicillin and Streptomycin (Pen/Strep ...
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bioRxiv - Molecular Biology 2023Quote: ... the transiently transfected HEK293 cells were harvested using dissociation buffer TrypLE (Thermo Fisher Scientific) and binding assay buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and HEK293 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; TM4, HeLa: Gibco™ #41966 – high glucose ...
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bioRxiv - Bioengineering 2023Quote: The plasmid was transfected into HEK293 cells using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: The human embryonic kidney HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were cultured in DMEM/F-12 cell culture medium (Gibco, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells were cultured in Freestyle™ 293 expression medium (Gibco).
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bioRxiv - Neuroscience 2023Quote: ... The Flp-In T-REx HEK293 cell line was used (Thermo Fisher Scientific, R78007). Stably expressing cells were isolated by treating with 200 µg/mL hygromycin B (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Reverse transfection was used to transiently transfect HEK293 cells using Lipofectamine 3000 (Thermo Fisher), using the method provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The Flp-In HEK293 cells were cultured in high glucose pyruvate medium (Gibco, #10569010), supplemented with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Tetracycline-inducible cells (tetON cells) were made in HEK293 Flp-In TReX cells (Invitrogen) (gift from Nancy Maizels) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells and RAW264.7 macrophages were cultured in DMEM/F12 (cat:11320082, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The experimental setup utilized an inducible T-REx HEK293 cell line (Thermo Fisher Scientific) expressing MC4-R and the inwardly rectifying K+ channel Kir7.1 mutant M125R ...
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bioRxiv - Plant Biology 2020Quote: ... The limit and efficiency of each primer pair of both target and reference gene was allowed to measure accurate comparison of gene expression using the 2-ΔΔCT method for relative quantification (the Applied Biosystems User Bulletin No. 2 (P/N 4303859)) (Livak Schmittgen ...
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bioRxiv - Cell Biology 2020Quote: ... N-ethylmaleamide (NEM, Acros Organics), DAPI (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... N-Dimethylformamide (DMF, Fisher Scientific) and swollen in 50% DCM/DMF for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... N-Diisopropylethylamine (DIPEA, Fisher Scientific) for 2 h at RT each ...
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bioRxiv - Biochemistry 2020Quote: ... Cy3 N-hydroxysuccinimide ester (Invitrogen) was dissolved in DMSO and stored at −20°C ...
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bioRxiv - Cancer Biology 2020Quote: ... GSE6532 ((n= 293, platform: Affymetrix Human Genome U133A and B Array ...
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bioRxiv - Cell Biology 2021Quote: ... on Vitronectin-N (Life Technologies) and passaged using TrypLE dissociation reagent (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... N-ethylmaleimide (Thermo Scientific, 23030).
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bioRxiv - Cancer Biology 2023Quote: ... N-Cadherin (Invitrogen, 33-3900); OXPHOS panel (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... N-hydroxysulfosuccinimide (Sulfo-NHS, ThermoFisher) were added on day 2 of the differentiation and included every media change ...
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bioRxiv - Cancer Biology 2023Quote: ... 100% n-propanol (Fisher Scientific) incubation for 1hr ...
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bioRxiv - Biophysics 2023Quote: ... mouse monoclonal anti-SARS-S2 (ThermoFisher, cat: MA5-35946, conjugated to HRP), goat anti-human IgG (sc-2453 ...
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bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Biochemistry 2020Quote: ... 3ul of anhydrous ACN and 4 ul of TMT reagent (32ug of TMT reporter tag) of each TMT reporter tag (TMT 10 plex kit, Product number: 90110 and A37725., Thermo Fisher Scientific) was added to each sample and incubated at room temperature on a Thermo mixer with a gentle agitation (600 rpm ...
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... using DMEM (4.5 g/l glucose) with 2 mM Glutamax-I medium supplemented with 15% foetal calf serum (FCS) (ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies, 11360-039] ...
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bioRxiv - Cancer Biology 2021Quote: ... FC-1242, and FC-1245)(Engle et al., 2019) were cultured in 10% FBS (Biowest) and 1% Anti-Anti (Gibco) in DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
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bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg of Fc control and CLEC18A-Fc fusion were incubated with AlexaFluor647 rabbit anti-mouse and goat anti-rabbit IgG (Invitrogen) at a molar ratio of 4:2:1 in array PBS and preincubated on ice for 10 mins at a total volume of 65 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455 ...
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bioRxiv - Genetics 2021Quote: ... Liver TAGs (Infinity Triglycerides Liquid Stable Reagent; Thermo Scientific) were determined following tissue lipid extraction in chloroform:methanol (2:1) ...