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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... then Alexa Fluor Donkey anti-sheep 594 secondary (1:250—Life Technologies #A-11016), along with DAPI and phalloidin as described above.
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bioRxiv - Immunology 2021Quote: ... hepaticus was inoculated on blood agar plates (TSA with 5% sheep blood, Thermo Fisher) and cultured in a hypoxia chamber (Billups-Rothenberg ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... GR1 and MAC1 in combination with sheep anti-rat IgG Dynabeads (Thermo Fisher Scientific) prior to the specific antibody staining ...
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bioRxiv - Molecular Biology 2021Quote: ... was conjugated to Dynabeads M-280 sheep anti-mouse IgG beads (Invitrogen, catalog #11201D). For FLAG ChIP-seq (for Brn1 and Hho1) ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen, Carlsbad, California, United States) per sample were washed twice in 1x BrU-IP buffer (20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... and Lin+ cells were depleted with sheep anti-rat IgG immunomagnetic beads (Dynabeads, Invitrogen). Cells were then immunostained and Lin- Flk2+CD27+ BM progenitors from F1 congenic strains were FACS purified (Fig ...
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bioRxiv - Microbiology 2019Quote: ... United Kingdom) and Alexa Fluor 488 donkey secondary antibody against sheep (#A11015, Life Technologies). Images of in situ PLA and immunofluorescence experiments were taken with a Zeiss Axioskop 40 fluorescence microscope using an AxioCam MRc 3 CCD sensor and 40x or 100x objectives with filters for DAPI ...
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bioRxiv - Genomics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG beads (Thermo Fisher Scientific, Cat. No. 11203D) were used for immunoprecipitation ...
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bioRxiv - Molecular Biology 2019Quote: ... pre-coupled to magnetic beads (Dynabeads M-280 Sheep anti-Rabbit IgG, Thermo Fisher). A series of wash steps was employed to ensure stringency ...
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bioRxiv - Physiology 2021Quote: ... Samples were then incubated with sheep anti-goat Alexa Fluor 594 (Life Technologies, A11058), or goat anti-mouse Alexa Flour 488 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... or donkey anti-sheep Alexa-Fluor-488-conjugated antibodies were used (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... supernatants were immunoprecipitated at 4°C overnight with Dynabeads Sheep anti–Mouse IgG (Invitrogen) conjugated with an anti-FLAG antibody (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... supernatants were immunoprecipitated at 4°C overnight with Dynabeads Sheep anti–Rabbite IgG (Invitrogen) conjugated with each antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Donkey anti-sheep IgG Cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen #A-11015) (1:700).
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bioRxiv - Molecular Biology 2022Quote: ... then for 1h at RT with prepared dynabeads sheep anti rabbit IgG (Invitrogen, #11203D). Coprecipiate was washed once with wash buffer (polysomal buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... the section incubated with Rabbit anti-Sheep secondary antibody (Thermofisher, Cat#: PI31240, 1-500), and the signal was detected using Leica Refine RED Kit (LeicaBiosystem ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetic beads (11204D, Dynabeads M-280 sheep anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific) were pre-blocked with BSA (1 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetic beads (11204D, Dynabeads M-280 sheep anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific) were pre-blocked with BSA (1 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa 488-conjugated donkey anti-sheep IgG (1:300, Invitrogen, Carlsbad, CA, USA, A11015), Alexa 488-conjugated donkey anti-mouse IgG (1:300 ...
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bioRxiv - Microbiology 2023Quote: ... solfataricus Alba antiserum as primary antibody with donkey anti-sheep IgG Alexa488 (Thermo Fisher) as secondary antibody ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A11016, Life Technologies) for 2h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding mouse TCR 2B4 α (with c-Myc-tag) and β (with V5-tag) sequences with 2A sequence linking each other were cloned into pCDNA3.1/Zeo(+) vector (Life Technologies) using Not1 and Xho1 restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in 1 mL PBS and 100 µL of Phos-Tag lysis buffer was added dropwise to the well (Phos-Tag lysis buffer recipe: RIPA (ThermoFisher 89900), 3X Halt protease and phosphatase inhibitor cocktail (ThermoFisher 78444) ...
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bioRxiv - Molecular Biology 2022Quote: Constructs encoding harmonin domains were cloned in the pDEST17-vector (Gateway cloning system, Invitrogen, USA). The cDNAs encoding C-terminal tails of Mm JAM-B tail (amino acids 257-299) ...
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bioRxiv - Molecular Biology 2020Quote: ... Plus3 domain point mutations were introduced using the Phusion Site-Directed Mutagenesis kit (ThermoFisher Scientific) and verified by sequencing.
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bioRxiv - Immunology 2024Quote: The bacterial codon-optimized coding sequence of ZBP1-Zα1Zα2 domains was synthesized (Thermo Fisher Scientific) and subcloned into N terminally His-Tagged pNIC-ZB vector ...
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bioRxiv - Biochemistry 2023Quote: ... and cDNA encoding GH1D1 domain was subcloned to a modified pET-32a vector (Invitrogen, USA) with an N-terminal TrxA tag and a 6×His tag followed by a TEV protease recognition site ...
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bioRxiv - Biophysics 2023Quote: Lphn3 GAIN domain constructs were expressed in Expi293 human embryonic kidney cells (Thermo Fisher, A14527) in suspension culture in Expi293 media (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...
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bioRxiv - Microbiology 2022Quote: ... and WIV1 spikes were synthesized (Integrated DNA Technologies) and cloned into pcDNA4TO (Invitrogen) in the form of ΔC19(10 ...
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bioRxiv - Molecular Biology 2021Quote: ... including the ERCC RNA Spike-In Mix-1 kit (Thermo Fisher Scientific, 4456740) that we used ...
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bioRxiv - Molecular Biology 2019Quote: External RNA Controls Consortium (ERCC) Spike-in (set 1) (Thermo Fisher Scientific, USA) was used as primary template24 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA samples were spiked with ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Systems Biology 2021Quote: ... we added 1uL of 1:5000 ERCC spike-in mix 1 (ThermoFisher #4456740) to every other column of wells on the plate ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Spike protein was labelled with Alexa Fluor™ 546 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... in-house produced trimerized spike protein-coated plates (Thermo Fisher Scientific, Roskilde, Denmark) followed by a 100 mM formic acid elution step ...
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bioRxiv - Genomics 2022Quote: ... External RNA controls consortium (ERCC) RNA spike-in mixes (Thermo Fisher Scientific, 4456740) were included for quality assurance ...
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bioRxiv - Genomics 2020Quote: ... We used External RNA Controls Consortium (ERCC) spike Mix 1 (Thermo Fisher, 4456740) [24] instead of ArrayControl RNA spikes.
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bioRxiv - Systems Biology 2022Quote: ... the RT reaction was performed in presence of ERCC RNA Spike-in (Ambion). Unused RT primers were digested using Exonuclease I (NEB ...
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bioRxiv - Systems Biology 2019Quote: ... 1: 5 000 000 ERCC RNA Spike-In Mix I (Thermo Fisher Scientific) in RNase-free water ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of a 1:50 dilution of ERCC Spike-In mix (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 100 ng of total RNA was combined with Array Control RNA Spikes (Ambion) and labelled using the Low Input Quick Amp WT Labelling kit (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... 3.75 µg psPAX and 1.5 µg spike containing plasmid using lipofectamine 3000 (Thermofisher). 4 x 106 cells were plated 16-24 h prior to transfection ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and reverse transcribed to cDNA with ERCC spike-in controls (Thermo Fisher Scientific). cDNA was amplified for 20 cycles using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) ...
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bioRxiv - Microbiology 2021Quote: Codon optimised open reading frames encoding spike proteins were synthesised (GeneArt, Thermo Fisher) and cloned into pCDNA3.1 and/or pD603 (ATUM ...