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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS; SAFC Biosciences) and 1% (v/v) Penicillin/Streptomycin (P/S; Gibco, Life Technologies). Primary fibroblasts were maintained in F-DMEM culture medium comprising DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... transferred to a new 2.0 mL Eppendorf tube and S-Trap columns incubated with 150 µL trypsin enzyme digestion buffer (1:30 trypsin enzyme (Thermo Fisher Scientific): protein by weight ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to a heating ramp from 25°C to 95°C at a rate of 1°C/s using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, USA). Data was analyzed by Protein Thermal Shift Software v1.4 to obtain melting temperature (Tm).
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bioRxiv - Cancer Biology 2024Quote: ... resuspended at a concentration of 1x104 cells per mL in complete RPMI 1640 cell culture media (10% FBS, 1% P/S) with 50 μM β-mercaptoethanol (Gibco 21985023). 100 μL of this cell suspension was added to each well such that each tumoroid initially started with approximately 1x103 cells ...
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bioRxiv - Systems Biology 2023Quote: Human induced Pluripotent Stem Cells were cultured in mTeSR Plus Medium (100-0276 Stem Cell) + Penicillin/Streptomycin (P/S) 1% (15140122 Thermo Fisher Scientific) on Matrigel coating (354277 Corning) ...
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bioRxiv - Molecular Biology 2023Quote: ... Starch samples were coated with 10 nm gold particles with 15 ma current for 1 min using Quorum Q150T and imaged using Apero S (Thermo Scientific, USA). To characterize the starch extracted from Arabidopsis rosette ...
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bioRxiv - Biophysics 2023Quote: ... Cells were rinsed with 1ml Leibovitz’s medium (Thermofischer, 21083027) supplemented with 10% Foetal Bovine Serum and 1% Penicillin Streptomycin or Fluorobrite DMEM imaging medium (Life Technologies, Thermofisher) and incubated 15 min at 37°C with 1 μM Flipper (same concentration and incubation time for all Flippers) ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... both supplemented with 10% heat-inactivated fetal bovine serum - FBS (Hyclone, USA) and 1% penicillin-streptomycin - P/S (10,000 U/mL; Gibco, Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2024Quote: ... The mouse cardiac fibroblast cell line L929 (ATCC, VA, USA) were cultured in RPMI 1640 medium containing 10% FBS and 1% penicillin/streptomycin (P/S, Gibco, CA, USA) at a 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and P-S solution (100 U/mL penicillin: Panreac, AppliChem GmBH, Darmstadt, Germany; 100 µg/mL streptomycin: Alfa Aesar, Thermo Fisher Scientific, Kandel, Germany (P-S) at 37◦C under a 5% CO2 atmosphere.
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bioRxiv - Microbiology 2021Quote: ... denaturation for 2 min at 95 °C followed by 45 cycles of 3 s at 95 °C and 30 s 60 °C on the StepOnePlus™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). All samples were run in triplicate ...
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bioRxiv - Genomics 2020Quote: ... Microbiopsies were collected in 96-well plates and lysed using the Arcturus PicoPure Kit (Applied Biosystems) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Amplicons were normalized using the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and eluted in 20 μL Elution Buffer ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction was normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001), then pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and normalized by using SequalPrep normalization plate kit (Invitrogen, CA, USA). Pyrosequencing was conducted on an Illumina MiSeq v2.2.0 platform through services provided by the UConn MARS facility.
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bioRxiv - Microbiology 2024Quote: ... They were than pooled and purified with the SequalPrep Normalization Plate kit (Invitrogen, Frederick, MD, USA). Sequencing was performed by GenoScreen (Lille ...
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bioRxiv - Microbiology 2024Quote: ... All samples were then cleaned with SequalPrep Normalization plate kit 96-well (Invitrogen, Carlsbad, CA, USA), pooled and sequenced on MiSeq (Illumina ...
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bioRxiv - Immunology 2024Quote: ... The following day PlatE cells were transfected with pBMN-plasmids using a Lipofectamin 3000 kit (Invitrogen) according to the manufactures protocol ...
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bioRxiv - Systems Biology 2023Quote: Amplicons from each 96-well plate were pooled and purified using the PCR Purification Kit (ThermoFisher). Each pooled sample was prepared as a single sequencing library by the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified and normalized using the SequalPrep normalization plate kit (Thermo Scientific, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg clarified mitochondrial lysates prepared in lysis buffer was incubated overnight with 50 µl glutathione-conjugated magnetic agarose beads (Thermo Scientific) at 4°C on a spinning rotor ...
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bioRxiv - Biophysics 2020Quote: ... and unprocessed H57-scFV (still containing a 12x histidine tag) were removed by a batch purification with Ni-NTA agarose (ThermoFischer Scientific) and glutathione agarose (ThermoFisher Scientific). The supernatant containing biotinylated H57-scFV was further purified via gel filtration using Superdex 75 (10/300 GL ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein fractions were site-specifically biotinylated using the BirA (Acro Biosystems Inc.) and subjected to a batch purification step with glutathione agarose (ThermoFisher Scientific) to remove the BirA ligase and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were dialyzed in equilibration buffer (50 mM Tris, 150 mM NaCl, pH 8.0) and incubated with Pierce® Glutathione Spin Columns (Thermo Scientific) at RT for 1 hour with gentle rotation according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Bacterial lysate was clarified by centrifugation at 40,000 xg for 45 min at 4°C and the clarified protein bound to glutathione agarose beads (Thermo Fisher Scientific) at 4 °C for 4 hours with rotation ...
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bioRxiv - Molecular Biology 2023Quote: BJ human skin fibroblast cells were grown in a humidified 37°C incubator containing 5% CO2 in MEM medium containing glutathione and high concentrations of vitamins (catalog no. 11875093, Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... The protein mixture was left on ice for 15 min then incubated with binding buffer-equilibrated glutathione agarose beads (Invitrogen, G2879) on a rotator for 1h at 4 ℃ ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with Penicillin/Streptomycin (P/S) (500 U/mL) (Gibco, 15140122) and 1% amphotericin B (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA oligos (Ambion, Life Technologies; siRNA ID #s s18158 and s18160) were transfected with Lipofectamine 3000 twice ...
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bioRxiv - Microbiology 2022Quote: ... blotted for 2 s using a Vitrobot Mk IV (Thermo Scientific) and applying the following parameters ...
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bioRxiv - Biophysics 2019Quote: ... Cells were incubated with 0.01% thioflavin S (Acros Organics, Waltham, MA) for 10 min at 37 °C and then washed with PBS ...
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bioRxiv - Genetics 2019Quote: ... ExpiCHO-S cells were purchased from Thermal Fisher Scientific (Life Technologies Corporation ...
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bioRxiv - Microbiology 2021Quote: ... 2 µM DSM1,79 6 µM blasticidin-S (Invitrogen Life Technologies R21001), 5 nM WR99210 (Jacobus Pharmaceuticals) ...
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bioRxiv - Microbiology 2021Quote: ... 2 µM DSM1,79 6 µM blasticidin-S (Invitrogen Life Technologies R21001), 5 nM WR99210 (Jacobus Pharmaceuticals) ...
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bioRxiv - Biophysics 2020Quote: ... Suspension Chinese hamster ovary cells (FreeStyle CHO-S cells, R80007, Thermofisher) were kept in suspension in plastic Erlenmeyer shake flasks (Corning ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1x penicillin and streptomycin (P/S, 100 U/ml, Gibco). DLD1 WT and DLD1 BRCA2-/- were purchased from Horizon Discovery and cultured in RPMI 1640 with GlutaMAX media and supplemented with 10% FBS and 1x penicillin-streptomycin (P/S) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were then transfected into ExpiCHO-S™ cells (ThermoFisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were then transfected into ExpiCHO-S™ cells (ThermoFisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... and 100 U/mL penicillin/streptomycin (P/S, Gibco, cat.no. 15140122).
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bioRxiv - Cell Biology 2021Quote: ... stained with HistoGene Staining Solution for 20 s (ThermoFisher Scientific, KIT0401) and washed again in water for 25 s ...
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bioRxiv - Microbiology 2020Quote: ... S-layer17 Cells were biotinylated with biotin-NHS-ester (21330, ThermoFisher) and incubated for 15 min at R.T ...
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bioRxiv - Biophysics 2022Quote: ... blasticidin S HCl and hygromycin B were purchased from Fisher Scientific. TurboFect™ transfection reagent ...
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bioRxiv - Molecular Biology 2022Quote: ... CHO-S cells (Cat nr R80007, Thermo Fisher Scientific, Waltham, MA) were adapted to grow in suspension in FectoCHO medium (Polyplus transfection ...
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bioRxiv - Immunology 2022Quote: ... The DNA construct was then transfected into CHO-S cells (Invitrogen) and selected with puromycin and methotrexate ...
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 10 s using a Vitrobot Mark IV (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Cell Biology 2022Quote: ... 15 µg/ml blasticidin S (Gibco, R21001; Invivogen, ant-bl-10p), 100 µg/ml hygromycin B (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor® 568 conjugate (Thermo Fisher Scientific Cat#S-11226), Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2020Quote: ... which were previously coated with streptavidin (Thermo Fisher Scientific #S-888) at 2 μg/ml and blocked ...
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bioRxiv - Immunology 2022Quote: ... + 10% FBS + P/S + Fungizone (Gibco™ by Life Technologies™) + HEPES (Sigma Aldrich ...