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Citations for Thermo Fisher :
2651 - 2700 of 4555 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Microbiology 2022Quote: His-GspD and His-PilQOlut were expressed in Escherichia coli BL21 cells and purified according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD ...
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bioRxiv - Immunology 2022Quote: The extracellular region of the HLA-DPA1*02:01 and HLA-DPB101:01 chains were separately cloned into pMT\BiP\V5-His A (ThermoFisher scientific). The alpha chain construct harbors the acidic leucine zipper and terminates by a 6His-tag ...
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bioRxiv - Plant Biology 2022Quote: ... Each sgRNA was introduced into the shuffle vectors by overlap PCR with Q5 Hi-Fidelity polymerase followed by digestion of the original vector with DpnI (Thermo Scientific). A destination vector harboring UBQ10 promoter ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS and 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Immunology 2023Quote: ... monocytes/macrophages (CD3-CD19-NKp46-CD11b+Gr1loLyC6int/hi) and neutrophils (CD3-CD19-NKp46- CD11b+Gr1+LyC6int) were counted using a Countess II Automated Cell Counter (Thermo Fisher) and then combined and processed as one sample per mouse ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were digested with EcoRI and XbaI and inserted into the mammalian expression vector pcDNA-V5-HIS-B (Invitrogen Corp.) that had been cut with the same enzymes ...
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bioRxiv - Microbiology 2024Quote: ... LGTV RNA was quantified with qPCRBIO probe mix Hi-ROX (PCR Biosystems) and primers recognizing NS372 on a StepOnePlus real-time PCR system (Applied Biosystems).
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% heat- inactivated fetal bovine serum (HI FBS) (Hycultec GmbH, Beutslsbach, Germany) and 100 IU/ml penicillin–streptomycin (Life Technologies). Cells were maintained at 37 °C under 5% CO2 humidified atmosphere ...
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bioRxiv - Immunology 2024Quote: Mouse PfTrxECL antisera was absorbed against TbpB-LCLEmpty using Dynabeads™ (CAT# 10103D, 10104D) according to the His-Tag Isolation & Pulldown protocol from Invitrogen. ECL-specific Abs were then assessed using Clear Flat-Bottom Immuno Nonsterile 96-well plates (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Plant Biology 2024Quote: ... Both input and pull-down samples were separated by 12% SDS-PAGE and analysed by immunoblotting with anti-GST antibody (1:2000, TransGen Biotech) or anti-his antibody (1:2000, Thermo Scientific).
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bioRxiv - Biochemistry 2024Quote: ... The resulting PCR products were utilized to clone pET-26b-Nanobody-ALFA-His in One Shot ccdB Survival 2 T1R Competent Cells (Invitrogen, A10460).
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bioRxiv - Biochemistry 2024Quote: ... for 1 h followed by incubation with 1:2,000 diluted 6x-His tag monoclonal antibody (produced in mouse; Invitrogen; #MA1-21315) at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... The His-tagged proteins were purified via immobilized metal affinity chromatography (IMAC) using HisPur™ Ni-NTA resin (Thermo Scientific, USA), then eluted in 50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2024Quote: 6xHis-Nβ products produced from enzymatic reactions with Notch variants were isolated by immunoprecipitation with Dynabeads His-tag Isolation & Pulldown (Invitrogen #10103) in 10 mM MES (2-(4-morpholino ...
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bioRxiv - Cancer Biology 2024Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Bioengineering 2024Quote: ... iBSCs were harvested and resuspended into serum-free B8 media containing Hi-Def B8 aliquots (Defined Bioscience #LSS-204, San Diego, CA, USA) in DMEM/F12 (ThermoFisher #11320032) supplemented with 1 % antibiotic/antimycotic and 2.5 μg/mL puromycin ...
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bioRxiv - Biochemistry 2024Quote: ... the purified labeled proteins were added together in 1x PBS at room temperature before adding them to Dynabeads Protein A labeled with either FLAG- or His- antibodies according to the manufacturer’s recommendations (LIFE Technologies, #10006D). First ...
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bioRxiv - Cell Biology 2024Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Cell Biology 2024Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Cancer Biology 2024Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Molecular Biology 2021Quote: ... Mass spectrometry was performed with an Applied Biosystems Voyager DE-Pro matrix-assisted laser desorption/ionization mass spectrometer from Life Technologies (Carlsbad, CA) in the Biophysics Instrumentation Facility at University of Wisconsin-Madison.
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bioRxiv - Biochemistry 2021Quote: ... LC-MS was performed by using a Velos Pro™ Hybrid Ion Trap-Orbitrap mass spectrometer (MS) coupled to a Dionex Ultimate 3000 HPLC (Thermo Fisher Scientific) (Schmitt et al. ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... Whole lanes of SDS-PAGE gel were cut out and subjected to LTQ Orbitrap Velos Pro ion-trap mass spectrometry (ThermoFisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Bioengineering 2021Quote: ... The tissues were homogenized with a 1000 µl digital pipette and a Bel-ArtTM Pro Culture Cordless Homogenizer Unit (Thermo Fisher, Waltham, MA). The samples were then centrifuged at 12,000 g for five minutes at 4°C to remove tissue debris with a Microfuge™ 22R centrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... The phosphorylation of SUMO-DsbR within the gel was detected by using a Pro-Q™ Diamond Phosphoprotein Gel Staining Kit (Invitrogen, catalog#: MPP33300) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: The 10 fractions obtained by high pH fractionation were analyzed using a nanoAcquity UPLC system (Waters GmbH) connected online to a LTQ-Orbitrap Velos Pro instrument (Thermo Fisher Scientific GmbH). Peptides were separated on a BEH300 C18 (75 μm x 250 mm ...
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bioRxiv - Microbiology 2020Quote: Vero cells were seeded at 1.2 × 104 cells per well in Opti-PRO™ SFM supplemented with 4 mM L-glutamine and 1X Antibiotic-Antimycotic solution (Gibco/Thermo Fisher Scientific) in black ...
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bioRxiv - Immunology 2020Quote: ... at a final concentration of 5×106 parasites per mL and incubated for 10 min with YO-PRO-1™ iodide at 0.1 μM final concentration (Thermo Fisher Scientitic, Y3603). Samples were then immediately analysed on the CytoFLEX cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was carried out using PerfeCTa® SYBR® Green SuperMix Reaction Mix (Cat. no. 95055-500) in real time PCR system Quant Studio 7 PRO (Applied Biosystems). For RT-PCR analysis of splicing ...
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bioRxiv - Biochemistry 2020Quote: N-glycans released from each granule membrane and soluble proteins were analyzed using capillary LC-MS/MS (Dionex Ultimate 3000) on an ESI linear ion trap mass spectrometer (LTQ Velos Pro, Thermo Scientific, Melbourne, Australia). Samples were injected onto a PGC-LC capillary column (Hypercarb KAPPA ...
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bioRxiv - Biochemistry 2020Quote: ... Urine metabolite separation and analysis was conducted using a Waters ACQUITY H-class LC system coupled with a LTQ-Orbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific, MA). The following 18-min gradient on a Waters HSS C18 column (3.0 × 100 mm ...
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bioRxiv - Genetics 2020Quote: A plasmid containing the landing pad construct was cloned using the 4 fragments in the MultiSite Gateway Pro Plus system (ThermoFisher, cat no. 12537100). The first donor fragment contained a 221 bp attP site and Caenorhabditis briggsae unc-119(+) ...
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bioRxiv - Cell Biology 2021Quote: ... they were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Synthetic Biology 2022Quote: ... we developed a vacuum-based plasmid extraction protocol named “TeVacS” on Fluent using the PureLink™ Pro Quick96 plasmid purification Kit (K211004A) (Invitrogen, Carlsbad, CA). Our customized protocol features the use of vacuum manifold for efficient removal of washing buffer and final elution of plasmid DNA into a 96-well plate placed on a 3D-printed holder ...
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bioRxiv - Developmental Biology 2020Quote: ... antibodies were diluted 1:500 in PBT with 1% DMSO and 1% BSA and applied together with TO-PRO-3 (T3605, Thermo Fisher, Waltham, MA) diluted 1:1000 over- night at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycosylation was detected by in-gel periodic acid-Schiff staining using the Pro-Q Emerald 488 glycoprotein staining kit (Invitrogen, Carlsbad, CA, USA) and imaged using a Typhoon FLA 9000 laser bed scanner (GE Healthcare Bio-Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptide mass was characterized by matrix assisted laser desorption/ionization (MALDI) on a Voyager DE Pro (Applied Biosystems Inc., Foster City, CA). The pure Def1 conjugate was air oxidized slowly with continuous stirring for 36-48 hrs and monitored the formation of S-S folding using MS/MS ...
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bioRxiv - Neuroscience 2022Quote: ... for 4 h and DAPI or TO-PRO® staining was used to visualize cytoarchitecture (1:5000 in PBS for 10 min; TO-PRO®: Invitrogen, #T-3605; DAPI: Thermo Scientific, #62248). Slices were washed ...
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bioRxiv - Systems Biology 2022Quote: ... and 1 µL of the samples were injected into a nano-ultra pressure liquid chromatography system (Dionex UltiMate 3000 RSLCnano pro flow, Thermo Scientific, Bremen, Germany) coupled via electrospray-ionization (ESI ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR was conducted using SYBR®Green Pro Tap HS(Accurate Biology,China)and detected by Step One Plus(Applied Biosystems,USA). Relative gene expression level = 2-ΔΔCt.ACTB (β-actin ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were analyzed on a LC-MS/MS system which consisted of an Orbitrap Velos Pro Mass Spectrometer (Thermo Scientific, Waltham, MA, USA) and a Dionex Ultimate-3000 nano-UPLC system (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... they were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Developmental Biology 2022Quote: LC/MS analysis was performed using an Orbitrap Eclipse Tribrid mass spectrometer equipped with FAIMS PRO coupled to an EASY-nLC 1200 system (Thermo Fisher Scientific Inc.). Peptides were resuspended in a mixture of 0.1% formic acid and 2% acetonitrile (v/v) ...