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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Recombinant Mouse Bace1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Immunology 2021Quote: ... The RBD-6xHIS protein was purified by affinity chromatography of His Tag residues for metals immobilized on the AktaPure system with HisPur ™ Ni-NTA Chromatography Cartridges (Thermo Fisher) column according to the manufacturer’s instructions ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: The fluorescence-labeled AFP1-His proteins were prepared according to the manufacturer’s instruction of the DyLight 550 Antibody Labeling kit (Thermo Fisher; Cat#84530). One OD600 of cells washed with MES buffer was subjected to immunostaining using the primary anti-HA antibody and AF488-conjugated secondary antibody or wheat germ agglutinin conjugated antibody (WGA-A488 ...
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bioRxiv - Immunology 2021Quote: ... Samples were then plated in 96-well flat bottom plates at a ratio of 25:75 with CD45.1 splenocytes and stimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
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bioRxiv - Biophysics 2022Quote: ... The ScPif1-N-His protein was labeled by binding to a 6x-His Tag Monoclonal Antibody (500 nM) conjugated to a DyLight™ 550 Fluorophore (Cat. # MA1-21315-D550, ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Immunology 2022Quote: ... and cloned Pst I/Not I in a pPPI4 expression vector containing a hexahistidine (his) tag with Gibson Assembly (Thermo Fisher Scientific). All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK ...
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bioRxiv - Immunology 2022Quote: ... The libraries were sequenced on Ion Proton using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite Software v5.2.2 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... were expanded and maintained in MEMα (GibcoTM 22561021) supplemented with 10% heat-inactivated FBS (HI-FBS; LabTech FB-1001/500 or Gibco 10438-026) at 37°C ...
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bioRxiv - Physiology 2024Quote: ... and Esr2b (XM_020713365) cDNA was amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Each nucleotide sequence of the 5′-flanking region of ara and arb was retrieved from the Ensembl medaka genome assembly and analyzed for potential canonical ERE-like sequences using Jaspar (version 5.0_alpha ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
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bioRxiv - Plant Biology 2023Quote: The baculovirus containing either His-mCherry-HT1 or twin strep-mVenus-MPK12 was constructed using the Bac-to-Bac™ system (Thermo Scientific). Resulting baculoviruses were used to co-infect 600 ml of Sf9 insect cells in the shaker culture at the density of 2×106 cells/ml at approximate multiplicity of infection 5-10 ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2023Quote: The Lysobacter enzymogenes gasdermin and the Pedobacter rhizosphaerae system were both expressed under an arabinose-inducible PBAD promoter in the pBad/His A plasmid (Thermo Scientific V43001). The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610 ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate was cleared by centrifugation at 20,000 x g for 30 min at 4 °C and purified by using GE Healthcare His GraviTrap™ Kit (Cat. No. 45-002-016, Fisher Scientific). After elution ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies against ZNF524 were purified and enriched from the serum against this recombinantly expressed His-ZNF524 using the SulfoLink® Immobilization Kit for Peptides (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: The piggyBac piRNA target site was inserted into the middle of the V5 coding sequence in the pIB-V5/His vector (Thermo Fisher Scientific) by site-directed mutagenesis ...
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bioRxiv - Biophysics 2024Quote: ... Lysates containing Utrophin or α-actinin were loaded on a column packed with His-Pur Ni-NTA resin (ThermoFisher Scientific, Waltham, MA). The column was washed with 0.6 M NaCl ...
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bioRxiv - Developmental Biology 2024Quote: pMT-Reps-Flag and pMT-Reps-HA were generated by cloning Reps-RA isoform from FMO04419 (DGRC Stock 1612020) into pMT-V5-His vector (Invitrogen cat#V412020). pMT-Mnb-V5 was generated by cloning Mnb-RH isoform from FMO12028 (DGRC Stock 1639183 ...
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bioRxiv - Microbiology 2019Quote: ... goat-α-mouse-Alexa-488 and goat-α-mouse-Alexa-594 (Life Technologies). FISH was performed using a PNA probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy5 ...
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bioRxiv - Systems Biology 2024Quote: ... Incubations were performed with mouse anti-FLAG or mouse anti-V5 (ThermoFisher Scientific), and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and treated with 1x ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used were against mouse (Donkey anti-mouse, AF488; Invitrogen Cat#A21202) or goat (Donkey anti-goat AF488 ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Cell Biology 2019Quote: ... washed once with PBS and mixed with a total of 10 μg recombinant DNA in electroporation buffer R (Invitrogen). Cells were singularly electroshocked at 1600mV for 20 msec by placing the electroporation tip into the column filled by E2 buffer (following manufacturer specifications) ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were cultured for 9 to 12 days in an erythroblast enrichment medium [38] to expand the erythroblast population and 2.5×105 cells were transduced using recombinant Sendai viral vectors (Cytotune-iPSC 2.0TM, Thermofisher scientific), expressing the four reprogramming factors Oct4 ...
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bioRxiv - Microbiology 2019Quote: The PfCHC-2xFKBP-GFP soluble extract in buffer B was incubated with 4μl recombinant anti-GFP nanobodies covalently coupled to surface-activated Epoxy magnetic beads (Dynabeads M270 Epoxy, ThermoFisher) for 1 hour ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were purchased from Kerafast and grown in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant epidermal growth factor (Gibco, #37010022) with 1% Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant lentiviral and retroviral vectors produced from previous steps were amplified using the GeneJET Plasmid Maxiprep Kit (Thermo Scientific). The 3rd generation lentiviral (pRSV.REV ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... containing the two recombinant Gateway sites attB1.1 and attbB.1 and subcloned into a pDONR/Zeo Gateway Donor vector (Invitrogen) as the entry clones ...
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bioRxiv - Cell Biology 2021Quote: ... Ift81 and Wdr35 were amplified by PCR with oligos carrying the recombinant sites and cloned into pcDNA3.1(+) vector (Thermofisher) in which a FLAG or HA epiptope was introduced prior to the multicloning site ...
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bioRxiv - Systems Biology 2022Quote: ... and recombinant synthetic genes fused to the GFP-coding sequence at the 3-termini were ordered from GeneArt (ThermoFisher). Genes were further cloned into pDUAL-PuroR lentivectors (Escors et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Cell Biology 2022Quote: All recombinant condensin complexes were expressed using insect cell strains from the Bac-to- Bac Baculovirus Expression System (ThermoFisher) as previously described (Kinoshita et al. ...
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bioRxiv - Plant Biology 2021Quote: The recombinant vectors were then transferred to Agrobacterium tumefaciens ElectroMAX™ LBA4404 cells by electroporation (Invitrogen, Carlsbad, CA, USA). The expression vector in A ...
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bioRxiv - Immunology 2020Quote: ... and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells ...