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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Recombinant Mouse Bace1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-H3K4me3 (Invitrogen). Secondary antibodies were purchased from Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse depletion Dynabeads (Invitrogen) were used for the depletion of lineage-labeled cells using a DynaMag-15 magnet (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-GFP (Invitrogen) 1/50 ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse anti-Pgk1 (Thermofisher). For quantitative Western blot analyses ...
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bioRxiv - Neuroscience 2024Quote: ... and GFP (mouse; Invitrogen). Appropriate fluorescent Alexa Fluor secondary antibodies were purchased from Invitrogen.
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bioRxiv - Synthetic Biology 2024Quote: ... mouse monoclonal antibody (Invitrogen). Following primary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Biochemistry 2019Quote: ... Site-directed mutagenesis of plasmids to generate the C-terminal Myc tagged ECH1 catalytic mutants and the C-terminal V5 tagged UCP3 truncation mutants was carried out with Platinum pFX DNA polymerase from (Life Technologies, Grand Island, NY), following the manufacturer’s protocols for site-directed mutagenesis ...
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bioRxiv - Genomics 2022Quote: ... we performed end repair and then selectively purified biotin-tagged DNA using streptavidin coated beads (DYNAL™ MyOne™ Dynabeads™ Streptavidin C1, Invitrogen 65001). A-tailing and Illumina TruSeq adapter ligation (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Molecular Biology 2022Quote: Resting primary B and T cells were isolated from mouse spleens by negative selection using Mouse CD43 (Untouched™ B Cells) and Untouched Mouse T Cells Dynabeads respectively (Invitrogen). For experiments done in quiescent splenocytes ...
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bioRxiv - Systems Biology 2020Quote: ... An Ion Torrent PGM was used with the Hi-Q 400 template preparation and sequencing kit and a 318v2 sequencing chip (Thermo Fisher, MA). All the quality control steps were performed using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: The pFastBacNKI-his-3C-m6A-Tracer construct was used for creation of baculovirus according to the Bac-to-Bac system (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... of 8 mice were then resuspended in 10 ml DMEM supplemented with 10 % heat inactivated new born calf serum (HI NCBS, LifeTechnologies / Gibco, # 26010-074) and plated on two 10 cm dishes (Corning ...
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bioRxiv - Genetics 2021Quote: One microliter of GlobalFiler™ or GlobalFiler™ Express amplified product was added to 9.5 μL Hi-Di™ formamide (ThermoFisher Scientific) and 0.5 μL GeneScan™ 600 LIZ® size standard (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the SBT3.8 ORF was amplified from genomic DNA with a reverse primer including six His codons and first cloned into pCR2.1-Topo (Life Technologies, Carlsbad, CA). EcoRI sites from pCR2.1 were used for ligation into pART7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were then pooled and loaded onto chips and sequenced on Ion Torrent PGM using Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific). Fast-q or BAM files from sequencing runs were analysed in Geneious ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2019Quote: ... PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen, Carlsbad, CA, USA). CD16158F-CR and CD32131R-CR were subcloned into NcoI and MluI sites of the SFG retroviral vector ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Genetics 2019Quote: ... using 1 μl PCR product in 9.5 μl Hi-Di™ formamide containing 0.5 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: The Taq polymerase-amplified PCR product of the rdLRP was inserted into the TOPO Cloning site of a pcDNA3.1/V5-His-TOPO vector (Thermo Fisher Scientific, MA). The vector was transfected to 6×105 cells of HEK293T cells using Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The full-length OpIE2 promoter was obtained from the pIB/V5-His by PCR using Phusion High fidelity DNA Taq polymerase (ThermoFisher Scientific, USA) according to the following program ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Molecular Biology 2022Quote: ... The sequencing was performed on Ion 318 v2 chip and Ion Torrent PGM machine employing the Ion PGMTM Hi-QTM View Sequencing kit (Life Technologies, USA). All procedures were carried out according to manufacturer’s instructions and each run was expected to produce approximately 150,000 reads per sample ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
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bioRxiv - Neuroscience 2020Quote: ... The ORF thus PCR-amplified was cloned into the pcDNA™3.1/V5-His TOPO™ TA vector (ThermoFisher Scientific, Cat # K480040) following manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.5 μl of fivefold-diluted PCR product was mixed with 9.25 μl of Hi-Di formamide and 0.25 μl LIZ600 size standard (ThermoFisher Scientific, Waltham, MA, USA), denatured for 10 min at 95°C and then immediately cooled on ice ...
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bioRxiv - Cell Biology 2019Quote: ... and FLAG-dDYNLL1 (EcoRI/XbaI) fragments were inserted into the S2-cell expression vector pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020).
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bioRxiv - Molecular Biology 2019Quote: ... using 1 μl PCR product in 9.6 μl Hi-Di™ formamide containing 0.4 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were run on a 15% PAGE and developed using monoclonal anti-His antibody (Biospecs, BTL1010) with super signal west femto maximum sensitivity substrate (Thermo Scientific, 34095).
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal twin-Strep-10x His dual tag followed by a stop codon was synthesized using GeneArt Gene Synthesis service (Invitrogen, Carlsbad, CA) with SgfI and MluI restriction sites at the beginning and the end of the ORF sequence ...
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bioRxiv - Genetics 2020Quote: The PCR products were diluted 1:1 with milliQ water and 1μL of the diluted PCR product was added to a 384-format genotyping plate containing 4μL of a mix of Hi-Di™ formamide (Applied Biosystems™) and Genescan™ 600 LIZ™ dye size standard v2.0 (Applied Biosystems™ ...
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bioRxiv - Immunology 2021Quote: ... an HRV3C protease cleavage site, a TwinStrepTag and Hexa-His-tag (McLellan et al., 2020) was transiently expressed in FreeStyle293F cells (Thermo Fisher scientific) using polyethylenimine (PEI ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... and T2/Onc2.r1 5’-ATC TAT GGC TCG TAC TCT ATA GGC-3’ designed to amplify the pT2/Onc2 vector excluding the MSCV 5’ LTR minimal promoter and Lun-SD sequences using platinum Hi-Fi DNA Taq polymerase (Invitrogen #11304-028) in 1X Hi-Fi PCR buffer (Invitrogen #52045 ...