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Citations for Thermo Fisher :
2601 - 2650 of 5415 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 4°C and the protein was purified from the supernatant using Ni-NTA Agarose (Invitrogen). The beads were washed with His-SUMO buffer and protein was eluted with His-SUMO elution buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
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bioRxiv - Neuroscience 2020Quote: ... The purified DNAs were then in vitro transcribed using MEGAscript T7 Transcription Kit (Invitrogen, AMB13345), and the resulting transcripts were purified using MEGAclear-96 Transcription Clean-Up Kit (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were purified by tandem affinity chromatography using HisPur Ni-NTA resin (Thermo Fisher Scientific) followed by strep-tactin sepharose resin (IBA) ...
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bioRxiv - Cancer Biology 2020Quote: ... which were purified by band excision after polyacrylamide denaturing gel electrophoresis (Novex 6% TBE, Invitrogen). Quantification of libraries was performed by qPCR (KAPA SYBR ...
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bioRxiv - Cell Biology 2021Quote: ... Purified RNA was used to prepare cDNA using the GeneChip WT Plus kit (Thermo Fisher) and hybridized using Mouse Clariom S gene chips ...
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bioRxiv - Physiology 2020Quote: ... Biotinylated cell surface proteins were affinity-purified using NeutrAvidin-conjugated agarose beads (Thermo Fisher Scientific), and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... purified eIF2 (100 nM) was incubated with 100 nM BODIPY-FL-GDP (Thermo Fisher Scientific) in assay buffer (20 mM HEPES-KOH ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Purified mRNAs were transfected in HEK293T cells in 24-well plates using Lipofectamine MessengerMAX (ThermoFisher). 24 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of purified protein was estimated both by conducting BCA assays (Thermo Fisher 23225) and by comparing band intensities of purified protein samples with a dilution series of 2 mg/mL Bovine Serum Albumin standard (Thermo Fisher 23209 ...
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bioRxiv - Microbiology 2020Quote: ... The protein was purified from the clarified lysate using HisPur Ni-NTA resin (Thermo Scientific) and eluted with 250 mM imidazole ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then dried and purified using 96-well hypercarb PGC plates (Thermo fisher) according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... purified dsRNA was treated with Ambion™ DNase I (Thermo Fisher Scientific, Waltham, MA, USA) to remove remaining genomic DNA remained ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of the purified amplicons was measured using a Nanodrop 8000 spectrophotometer (Thermo Fisher) and their quality was checked using DNA 7500 chips onto a Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... and the resulting transcripts were purified using MEGAclear-96 Transcription Clean-Up Kit (Invitrogen, AM1909). The sgRNAs and Cas9 mRNA were injected into zygotes from C57BL6 ...
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bioRxiv - Genomics 2020Quote: ... Purified dsRNA was quantified on a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and analysed by agarose/TAE gel electrophoresis to confirm quality and predicted size.
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bioRxiv - Genetics 2021Quote: ... mRNA from the edited cells was purified using TRIzol RNA Isolation kit (Thermo Fisher Scientific) according manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The purified PCR product was used for Sanger sequencing on 3500 Genetic Analyser (Applied Biosystems).
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bioRxiv - Genomics 2021Quote: We purified genomic DNA (gDNA) using a Genomic DNA Purification Kit (Thermo Fisher Scientific, #K0512) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... plasmids were grown under ampicillin selection and purified using GeneJET plasmid Midiprep Kit (Thermo Scientific), following the protocol specified by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Amplified PCR products were purified using PureLink® PCR Purification Kit (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were purified and cloned into pENTR/D-TOPO vector (Thermo Fisher Scientific). The cDNA fragments were then cloned into the modified pER8 plasmid containing a Gateway cassette (pER8-GW)52 using the Gateway LR Clonase □ (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and the quality of purified RNA was assessed with Qubit (Thermo Fisher Scientific, Waltham, MA) and Bioanalyzer Pico chip (Agilent ...
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bioRxiv - Cell Biology 2021Quote: Native kinetochore particles were purified (see kinetochore purification assay) and kept bound to Dynabeads (Invitrogen). Beads were washed once with phosphatase buffer (Buffer H ...
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bioRxiv - Immunology 2021Quote: ... The libraries were gel purified and quantified using a Qubit high-sensitivity DNA kit (Invitrogen), and the library quality was confirmed using Tapestation High-Sensitivity DNA tapes (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was removed from purified RNA by adding 2 µL of Turbo DNase (Invitrogen) in a 100 µL final volume using the provided buffer and incubating 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... plasmids were purified using a Plasmid DNA Midiprep Kit (K210005, Thermo Fisher Scientific, MA, USA).
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bioRxiv - Biochemistry 2022Quote: ... The purified PCR fragments were cloned into the pMD18T vector (DH5α, Thermo Fisher Scientific, USA). The resulting DicVg4 plasmids were used as templates to generate DicdsVg4 with the RNAsyn dsRNA kit (RNAsyn ...
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bioRxiv - Immunology 2022Quote: ... cDNA was generated from the purified RNA using the M-MLV Reverse Transcriptase kit (ThermoFisher). qPCR analysis was performed using TaqMan primer/probe sets and master mix (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... The protein-digested exDNA was finally purified using GeneJET NGS Cleanup Kit (Thermo Scientific, USA). The purified exDNA isolates were stored at −20°C pending analysis.
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted and purified from cells directly lysed in TRIzol reagent (Life Technologies). For RT-qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were purified using C18 spin tips (Cat. No. 87784, Thermo Scientific, Waltham, MA) and solubilized in water containing 5% acetonitrile (ACN ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of the purified protein was determined using a Nanodrop One (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... Purified ARNT-3xFlag or ARNT2-3xFlag homodimers were then enriched using 100KDa mwco filters (Ambion), while removing some contaminants ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR purified products were ligated to the pCR™2.1-TOPO® vector (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 500 ug purified RNA was used for cDNA synthesis by Superscript III Reverse Transcriptase (Invitrogen). cDNA was diluted 1:3 in nuclease-free water (Ambion ...
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bioRxiv - Immunology 2022Quote: ... The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen). The beads were subsequently washed with 1X TBS and boiled with SDS-PAGE reducing buffer with β-mercaptoethanol ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was further purified using the TURBO DNA-free(tm) kit (ThermoFisher, Waltham, MA, USA) to eliminate genomic DNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR reactions were pooled and purified using the GeneJET PCR Purification Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... The plasmids were then extracted and purified using a PureLink Plasmid Mini Kit (Thermo Fisher) and sequenced (Genetic Analyzer ...
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bioRxiv - Immunology 2022Quote: ... The endotoxin levels of the purified antibodies were measured by Chromogenic Endotoxin Quant Kit (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The purified library was quantified with a Qubit 4 Fluorometer (Thermo Fisher Scientific, Waltham, MA) and quality checked with an Agilent BioAnalyzer 2100 for size and concentration and sequenced with Illumina MiSeq nano run (2 x 250 bp ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: Purified antibodies were labeled with Alexa Fluor 647 NHS ester (Thermo Fisher Scientific, Darmstadt, Germany) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... Whole cell lysates or purified PDHC reactions were separated on 4-12% Bolt Gel (ThermoFisher) after heat denaturation in sample buffer (ThermoFisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... treated and DNA was purified by phenol-chloroform extraction and precipitated with GlycoBlue (Thermo Fisher), sodium acetate (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Subsequently RT-PCR of the purified RNA was performed using OligodT20 Primer (18418020,Thermo Fisher) and SuperScript III Reverse Transcriptase (18080044 ...
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bioRxiv - Microbiology 2020Quote: ... The crude total RNA was further purified using Turbo DNA-free Kit (Thermo Fisher Scientific) to remove DNA ...
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bioRxiv - Cell Biology 2021Quote: ... equal volume of the total RNA extract was further purified with DNA-free Kit (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 1 µg of purified antibodies was conjugated to 10 µl Dynabeads Protein G (Thermo Fisher) for 30 min at room temperature ...