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Citations for Thermo Fisher :
2501 - 2550 of 5415 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was then purified using the KingFisher Flex magnetic particle processor system (ThermoFisher Scientific). Isolates from the CNM in Spain were grown in glucose-yeast extract peptone liquid medium with 0.3% yeast extract and 1% peptone (Difco) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA samples were isolated and purified using TRIzol (Thermo Fisher Scientific, Waltham, MA, USA). RNA was reverse transcribed to cDNA using SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then purified using the GeneJet PCR Purification Kit (Thermo Fisher Scientific), digested with BamHI and PstI restriction endonucleases according to the manufactureŕs instructions (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... and then the sgRNA was purified using the MEGAclear kit (Life Technologies, cat # AM1908) according to the kit protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified amplicons were cloned into pDONR/Zeo via Gateway BP Clonase II (Thermo Fisher) and transformed into E ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified 2nd step libraries were quantified using the Qubit dsDNA Quantification Assay Kit (ThermoFisher) and sequenced on either HiSeq4000 by PE50bp or NovaSeq6000 PE100bp with a 30% PhiX spike-in.
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragments and digested vectors were purified using GeneJET PCR Cleanup Kit (ThermoFisher, K0701) or GeneJET Gel Extraction Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA was purified with silane magnetic beads (DynaBeads MyOne Silane Beads, ThermoFisher Scientific). The purified cDNA was further amplified by PCR to generate sufficient material for library construction ...
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bioRxiv - Neuroscience 2024Quote: ... The mRNA was then purified using the DynabeadsTM mRNA purification kit (61006, Thermo Fisher). The ac4C dot-blot was performed according to the previous study19 ...
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bioRxiv - Cell Biology 2024Quote: ... The total RNA was purified with a PureLink RNA Mini kit (Thermo Fisher Scientific) and sent to Novogene for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using ExoSAP-IT (Thermo Fisher Scientific, Waltham, MA, USA) and sent for sequencing at Eurofins Genomics ...
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bioRxiv - Cancer Biology 2024Quote: ... The quality of purified RNA was measured by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) followed by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... the His-tagged protein was purified using the ProBond Purification System (Thermo Fisher Scientific) and the eluted protein was dialyzed against PBS ...
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bioRxiv - Microbiology 2024Quote: Total RNA was purified according to (92) followed by treatment with DNase I (Invitrogen). RNA was repurified via standard sodium acetate/isopropanol precipitation and quantified using a NanoDrop (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... purified and normalized using a SequalPrep Normalization Plate (96) Kit (ThermoFisher Scientific, Waltham, MA). Samples ...
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bioRxiv - Immunology 2024Quote: ... purified EVs in PBS were treated with Proteinase K (Thermo Fisher, 50 ng/µl) for 30 min at 37°C then quenched by incubation at 75°C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified DNA samples were quantified using the Qubit dsDNA high sensitivity assay (ThermoFisher Q32854) with the Qubit 4 fluorometer ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of the purified RNA was determined using a NanoDrop spectrophotometer (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Purified RPFs were then used to synthesize cDNA with SuperScript III (Thermo Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro transcribed RNAs were purified by NucAway™ Spin Columns (Invitrogen, Carlsbad, CA), and RNA concentration was quantified by spectrophotometer-SimpliNano (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: Crude RNA was purified by anion exchange chromatography (Thermo Scientific Ultimate 3000 HPLC System) on a semi-preparative Dionex DNAPac® PA-100 column (9 mm x 250 mm ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified RNA was reverse-transcribed into cDNAs with SuperScript® II reverse transcriptase (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... the reaction mixture was purified using Protein-A Sepharose affinity chromatography (Thermo Fisher Scientific), followed by dialysis in PBS at 4°C for 22h ...
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bioRxiv - Genetics 2024Quote: ... coli under appropriate conditions and purified using GeneJET plasmid miniprep kit (Thermo Fisher Scientific) using included instructions ...
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bioRxiv - Microbiology 2024Quote: ... The purified protein was quantified by Qubit™ 4 fluorometer (Invitrogen™, MA, USA). The molecular masses of recombinant protein were evaluated by 1-D sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... The final PCR products were purified by PureLink PCR purification kit (Thermo Fisher Scientific) and submitted for next-generation sequencing using Illumina MiSeq PE250.
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bioRxiv - Plant Biology 2024Quote: ... agrestis Rubisco large subunit C-terminus peptide EVWKEIKFVFETIDTL and affinity purified (Life Technologies, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific, Waltham, MA, USA). Purified plasmids were sequenced in both directions to confirm coding regions.
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bioRxiv - Evolutionary Biology 2024Quote: ... We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher) and Qubit fluorometer 3.0 (Thermofisher).
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bioRxiv - Cancer Biology 2024Quote: ... The purified PCR products were separated by a 4-20% TBE gel (Invitrogen; EC62252BOX) and stained with GelRed for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... Concentrations of purified protein were measured with the Pierce nano bicinchoninic acid assay (Thermo Scientific), as previously described (40 ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR amplified cDNA construct was purified using the GeneJET PCR Purification Kit (ThermoFisher Scientific) according to the manufacturer’s instructions and then separated on 6% polyacrylamide gel ...
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bioRxiv - Zoology 2021Quote: ... Samples containing visible bands were purified with exonuclease I and FastAP Alkaline Phosphatase (Thermo Scientific) and sequenced using a BigDye version 3.1 kit and ABI Prism 3130XL Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 ug of small RNA purified by the mirVana miRNA Isolation kit (Thermo Fisher Scientific) was heated at 90 °C for 20 sec with gel loading buffer II (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... 1.5 µg of purified RNA was used for reverse transcription using Superscript III enzyme (Invitrogen). Reverse transcribed RNA was subsequently used for conventional RT-PCR or qRT-PCR using Taq polymerase ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was purified from gel and cut with KflI (cat# FD2164, ThermoFisher Scientific) and SalI (cat# R3138 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified by tandem affinity chromatography using HisPur Ni-NTA resin (Thermo Fisher Scientific) followed by Strep-Tactin Sepharose resin (IBA) ...
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bioRxiv - Immunology 2021Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
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bioRxiv - Genetics 2021Quote: ... The mixes were then purified with BigDye X-Terminator™ Purification Kit (Thermo Fisher Scientific) and sequenced by capillary electrophoresis on an ABI 16-capillary 3130xl Analyzer (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... using autoinduction Luria-Bertani (LB) broth and purified using HisPur cobalt spin columns (Thermo Scientific). Proteins were purified under denaturing conditions according to the manufacturer’s protocol using 8 M urea ...
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bioRxiv - Plant Biology 2021Quote: ... The crosslinking was reversed overnight at 65 °C and purified DNA (Thermo Scientific, Cat# 17908) was used to generate libraries with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were gel-purified and cloned into the pCR2.1-Topo Vector System (ThermoFisher Scientific) and sequenced using the M13rev primer (5′-CAGGAAACAGCTATGAC-3′) ...
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bioRxiv - Developmental Biology 2021Quote: ... Amplicons were purified using GeneJET PCR Purification Kit (K0702, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s directions and cloned using CloneJET PCR Cloning Kit (K1231 ...
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bioRxiv - Developmental Biology 2020Quote: ... Complementary DNA was generated from purified RNA using High Capacity Reverse Transcription kit (ThermoFisher Scientific), diluted 1:10 ...
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bioRxiv - Immunology 2020Quote: Purified HSPC populations were labelled with CTV and cultured in RPMI 1640 media (Life Technologies) with or without freshly added Flt3L (BioXcell ...
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bioRxiv - Microbiology 2020Quote: ... The Fabs were purified with a CaptureSelect™ CH1-XL Affinity Matrix (Thermo Fisher Scientific) followed by size exclusion chromatography.
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bioRxiv - Cancer Biology 2022Quote: ... RNA was purified using UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) (Thermo Fisher), quantified with Nanodrop 2000c instrument (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... purified and cloned into Escherichia coli (TOPO™ TA Cloning™ Kit for Sequencing, Invitrogen) (Additional methods in Supplemental Information).
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bioRxiv - Microbiology 2022Quote: ... PCR products and restriction digest products were purified using GeneJET PCR purification kit (Thermo Fisher) per manufacturer protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of the purified RNA was determined using NanoDrop 2000 (Thermo Fisher Scientific, USA) and an equal amount of RNA from various samples was used for preparing cDNA using random hexamer primers according to the manufacturer’s protocol ...