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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Hepa 1-6 (Murine hepatoma) cells were grown in DMEM (Gibco; 11995-065) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by secondary staining for one hour at RT (1:250, Alexa-488, Invitrogen, A11008). Images were taken using a LSM 510 confocal microscope (Carl Zeiss ...
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bioRxiv - Biochemistry 2020Quote: ... One aliquot was treated with DSG (disuccinimidyl glutarate) crosslinker (1 mM) (Thermo Scientific, Cat# 20593) and the other served as a DMSO only negative control ...
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bioRxiv - Neuroscience 2021Quote: ... 60 degrees for 1 min] x 40 cycles) on a Step-One Plus (Applied Biosystems) using Sybr Green (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... and one guide sequence (Table 1) was generated by following the MEGAscript®Kit (Invitrogen) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were stained for one hour with phalloidin Alexa fluor 488 (1:300, ThermoFisher, A12379) or phalloidin Alexa fluor 568 (1:300 ...
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bioRxiv - Genomics 2024Quote: ... one third of the vegetative stage cells were resuspended in 1 ml TRIzol Reagent (Invitrogen) and stored at −20°C until further processing ...
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bioRxiv - Microbiology 2023Quote: ... RNA quality was verified by 1% agarose electrophoresis and quantified using Nanodrop One (Thermo Scientific). As control ...
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bioRxiv - Molecular Biology 2024Quote: ... and checked on 1% agarose gel and concentration measured with a NanoDrop One spectrophotometer (ThermoFisher). Double-stranded RNA targeting the gene encoding the green fluorescent protein (dsGFP ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... CB (1:1) and buffy coats (1:2) were diluted with sterile 1xPBS (Gibco by Life Technologies, California, USA) and MNCs were isolated by density gradient centrifugation (Biocoll ...
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bioRxiv - Immunology 2020Quote: ... CB (1:1) and buffy coats (1:2) were diluted with sterile 1xPBS (Gibco by Life Technologies, California, USA) and MNCs were isolated by density gradient centrifugation (Biocoll ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740).
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740). Libraries were prepared using the Kapa RNA HyperPrep Kit with RiboErase (Roche #08098131702) ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Immunology 2021Quote: ... EBM-2/EGM-2 medium was supplemented with puromycin (1 mg/ml, Gibco) for 3 d ...
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bioRxiv - Bioengineering 2019Quote: ... denaturing gels were run with 6 μL per well of a 1:1 mixture of sample and Gel Loading Buffer II with formamide (Ambion) in a 10% TBE-Urea ...
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bioRxiv - Physiology 2021Quote: ... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03% Pluronic F-127 (w/v) ...
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bioRxiv - Physiology 2022Quote: ... The prepared slices were stained in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03 % Pluronic F-127 (w/v) ...
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bioRxiv - Neuroscience 2022Quote: ... embryoid bodies were embedded in 15 µL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation media consisting of 1:1 DMEM-F12 and Neurobasal media (Gibco), with addition of 0.5% N2 supplement (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... per 1×104 SiHa and 1×103 HeLa cells/6-well culture dish were transfected with Lipofectamine RNAi-Max (Invitrogen). Efficient knock down was confirmed by western blot analysis ...
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bioRxiv - Cancer Biology 2019Quote: Viability was assessed after 8 weeks of culture by staining with 4 μM ethidium homodimer-1 and 2 μM calcein AM (Thermo Fisher Scientific). Any condition where at least one viable cell was identified was considered positive for the presence of viable cells ...
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bioRxiv - Epidemiology 2019Quote: ... for 2 h at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-myeloperoxidase (MPO, 1:100, Invitrogen, CA, USA) or rabbit monoclonal anti-F4/80 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... hMOs were then washed overnight with 1X PBS at 4°C and incubated with Hoechst 33342 solution in 1X PBS for 2 h (1:1000, Thermo Fisher Scientific) and washed in PBS for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The MTS were stained with a mixture of 2 μM calcein AM and 4 μM ethidium homodimer-1 in PBS (Thermo Fisher Scientific) to stain for live and dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... 45 s in air at 15 mA and 0.4 mbar) holey carbon grid (C-flat: 2/1-3C-T) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) after blotting for 3 seconds with blot force 18 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... S2P6 was serially diluted 1:4 starting at 80 μg/ml in infection media (DMEM supplemented with 2% FBS and 20mM HEPES (Gibco, 15630-080)) and incubated with replication-competent VSV-SARS-CoV-2 (23 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBS and incubated for 2 h at 4°C with appropriate combinations of AlexaFluor-conjugated secondary antibodies (Invitrogen,1:2000) for 2 hours at 4C protected from light ...
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bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed 4-5 times in PBS for 2 hours then incubated for 3-4 hours with secondary antibody (anti-rabbit Alexa Fluor 568; 1:1000; Invitrogen; Cat #A10042) and NeuroTrace Green Fluorescent Nissl Stain (1:2000 ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Immunology 2020Quote: ... and Fluo-4 AM (Invitrogen, 2 μM) probes for 30 min before fixation with 1% PFA for 4 hours ...
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bioRxiv - Genomics 2021Quote: ... 3.5× 10−4% 2-mercaptoethanol (Gibco, 21985023), 0.12% Nicotinamide (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... mouse HMB45 (1:20; 4°C overnight; Life Technologies, 081050). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg/mL of FM 4-64 Dye (ThermoFisher, T13320) was contained in agarose media ...
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bioRxiv - Biophysics 2022Quote: ... and 4 µM Oregon Green 488 BAPTA-1 (Fisher Scientific) in extracellular buffer for 30 minutes at 37 °C ...
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bioRxiv - Systems Biology 2022Quote: ... mixed in a 4:1 ratio with M199 (Thermofisher, 41150020), 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... containing 4% FCS and 1% L-Glutamine (L-Gln; Invitrogen). These adherent cells were cultured to confluence for five days at 37 °C in an atmosphere containing 5% CO2.