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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... containing 1% (v/v) 2-mercaptoethanol (Gibco #31350010) and 0.5 U/µL of SUPERase•In RNase Inhibitor (ThermoFisher #AM2694).
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bioRxiv - Biophysics 2021Quote: ... were grown in proliferation medium (DMEM-F12 GlutaMAX™ + 10% heat inactivated FBS + 1% non-essential amino acids +10 mM HEPES buffer + 1% Penicillin/Streptomycin; Gibco®, Invitrogen, USA). For microinjection the cells were seeded on glass bottom µ-Dishes with a cell location grid (grid-50 ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were diluted in 1 % non-fat dry milk in TBS-T: mouse anti-TFR1 antibody (1:1,000, Thermo Fisher Scientific, 13-6800, RRID:AB_2533029), rabbit anti-FPN antibody (1:2,000 ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Immunology 2023Quote: ... 7-Aminoactinomycin D (7-AAD) (Invitrogen) at 1:400 was used as a marker for dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Molecular Biology 2022Quote: ... All sections were mounted with 50μl of ProLong Gold Antifade mounting media containing a fluorescent nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, P36931) before imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were rinsed three times with 1X PBS and coated with ProLong Gold antifade mounting medium with DAPI (4’,6-diamidino-2-phenylindole, 100 ng/ml) (Life Technologies, P-36931) on slides ...
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bioRxiv - Bioengineering 2023Quote: ... These slices were then fixed in 4’,6-diamidino-2-phenylindole (DAPI) mounting media (Fluoromount-G® with DAPI, Thermo Fisher Scientific, USA), and washed twice with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then stained with 4′,6-diamidino-2-phenylindole (DAPI) to stain cell nuclei and mounted with Immu-Mount mountant (Thermo Scientific, Cat# 9990402) onto microscope slides.
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bioRxiv - Microbiology 2022Quote: ... and blocked for 45 min in 2% bovine serum albumin (BSA) in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Cell Biology 2021Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 x g for 1 hour, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was incubated with 2 ml Ni-NTA resin (Thermo SCIENTIFIC #88222 ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight and then loaded with Fluo-4 (1:1000; Fluo-4 Calcium Imaging Kit; Invitrogen) and CellTracker Red (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
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bioRxiv - Neuroscience 2020Quote: ... serum-free medium (DMEM:F12 at 2:1) supplemented with N2 (1% v/v; Invitrogen), 1mM L-glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated wildtype and Ncan KO cortical neurons were initially infected with Control and the two Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Biochemistry 2024Quote: ... Solutions were further diluted to 1 µM using 2:1 MeOH:CHCl3 (ThermoFisher Scientific, Australia) with 5 mM ammonium acetate (SigmaAldrich ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated cortical neurons were infected with Control and Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa647 ICAM-2 at 1:100 and Live/Dead at 1:1000 (L34962, Invitrogen), for sorting of CD45neg ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 2-mercaptoethanol and 1 mM PMSF) by sonication (using a Fisher Scientific Sonic Dismembrator with a Qsonica Ultrasonic Sonicator converter ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen, Cat. L3000015). Viral supernatants were collected 48 hours after transfection and filtered through a 0.45-μm membrane (Corning ...
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bioRxiv - Immunology 2020Quote: ... 2% MEM non-essential amino acids (MEM NEAA, Gibco, Paisley, UK, reference 11140-035), 50ug/ml gentamycin (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... MEM non□essential amino acid solution and 2□mercaptoethanol (all from Thermo Fisher, USA) supplemented with 20□ng/mL bFGF and 0.25□mM Sodium butyrate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM L-Glutamine and 0.1 mM Non-Essential Amino Acids (NEAA) (all Gibco). Cells were incubated at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: The following types of microbeads were used: non-fluorescent 1 µm sulfate/latex beads (ThermoFisher Scientific S37498), non-fluorescent 2 µm sulfate/latex beads (ThermoFisher Scientific S37500) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% non-essential amino acids in 75 cm2 culture flasks (NUNC™, Roskilde Site, Kamstrupvej, Denmark) under humidified standard condition at 37 °C in 5% CO2 atmosphere (Forma water jacketed CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... plus 10% fetal bovine serum and 1 × MEM Non-Essential Amino Acids Solution (Life Technologies, # 11140-050).
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bioRxiv - Immunology 2022Quote: ... enriched NK cells were fixed with 1 % formaldehyde (diluted in PBS from 16 %, non-methanol format, Thermofisher) for 20 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... antibiotics (100U of penicillin/mL and 100μg of streptomycin/mL) and 1% non-essential amino acids (Gibco). Baby hamster kidney– 21 (BHK-21 ...
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bioRxiv - Immunology 2021Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). After the first passage the antibiotic concentration was reduced to 1%.
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bioRxiv - Cell Biology 2020Quote: ... Unlipidated C25SShhN cDNA and non-palmitoylated C25AShh cDNA (amino acids 1-438) were inserted into pcDNA3.1 (Invitrogen). Primer sequences can be provided upon request ...
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bioRxiv - Microbiology 2023Quote: 1×105 HFFα fibroblasts nucleofected with US10- or non-targeting siRNA were seeded in DMEM (Life Technologies) supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1% v/v MEM Non-Essential Amino Acids Solution 100X (Gibco, Life Technologies).
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... and essential medium non-essential amino acids solution (diluted 1/100) (all from Invitrogen, Carlsbad, CA, USA). Cells were maintained at 37 °C in an atmosphere with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1% v/v MEM Non-Essential Amino Acids Solution 100X (Gibco, Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Unlipidated C25SShhN cDNA and non-palmitoylated C25SShh cDNA (amino acids 1-438) were inserted into pcDNA3.1 (Invitrogen). Primer sequences can be provided upon request ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1% minimum essential medium non-essential amino acids (MEM NEAA; Thermo Scientific, Gibco, Cat. #11140) and 1% Penicillin-Streptomycin (Thermo Scientific ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 1% minimum essential medium non-essential amino acids (MEM NEAA; Thermo Scientific, Gibco, Cat. #11140) and 1% Penicillin-Streptomycin (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 μg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...