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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and placed in a 6-well plate filled with 1 ml/well of culture media containing 50 % Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23 % EBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Cell Biology 2021Quote: ... HSC (Lin−Sca-1+c-Kit+CD150+CD48−) from wildtype C57BL/6 mice were sorted into 16-well chamber slides (Thermo Fisher Scientific) coated with RetroNectin (Takara Bio) ...
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bioRxiv - Cell Biology 2021Quote: ... total 2 μg was diluted into 6 μl of reagent (1:3 ratio) in the 100 μl of Opti-MEM I medium (31985062, Thermo Fisher Scientific) and incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... prior to washing (6×60 min PBST) and secondary incubation with species specific Alexafluor IgG secondary antibodies (60 min RT, Invitrogen; 1:500). Tissues were washed again in 0.1% PBST (6 × 60 min) ...
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bioRxiv - Developmental Biology 2022Quote: Mice were anesthetized using isoflurane and injected with 1% Biocytin-5(and-6-)- Tetramethylrhodamine (Biocytin-TMR: ThermoFisher Scientific; Waltham, MA, Cat# T12921). After 30 minutes in circulation ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Virus mutant libraries were rescued in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence in a T75 flask (75 cm2) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: sg-mEX3 or sg-mEX5 and SpCas9-2A-GFP plasmids at 1:1 molar ratio were co-transfected into ~80% confluence OC-k1 cells in 6-well plate using Lipofectamine 3000 (Thermo Fisher Scientific). The cells were incubated for 48 hours and then submitted for fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected after 6 days and passed through a gravity chromatography column containing 1 ml of PierceTM Protein G Agarose resin (ThermoFisher Scientific 20397). The column was washed with 20 ml of wash buffer (150mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... 4*105 TX1072 cells cultured in SL medium were reverse transfected with 250 ng pX330-PK45/46 and 750 ng of the pA cassette-containing donor plasmid (SP292) with Lipofectamine 3000 reagent using 1 µl P3000 reagent and 6 µl Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s guidelines ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... formation was induced by culturing cell aggregates in suspension from 1-well of a 6-well plate on a 35×10mm petri dish (Thermo Fisher Scientific) with mTeSR1 medium ...
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bioRxiv - Genetics 2020Quote: ... Confocal microscopy was carried out embedding the 6 dpf larvae in 1 % (w/v in E2 medium containing tricaine) low-melting agarose (Fisher Scientific, USA) in a 50-mm glass bottom dish (MatTek ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...
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bioRxiv - Biophysics 2022Quote: ... 1 x 106 Sf9 cells were transferred to each well of a six-wells plate and subsequently transfected with 1 μg of bacmid DNA by 6 μl of Cell-fectin (Thermo Fisher Scientific). Baculovirus containing cell supernatants (P1 ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 1 µg of the indicated plasmids (6-well plates) by using the Lipofectamine 2000 system (Thermo Fisher Scientific). For transient transfection with siRNA ...
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bioRxiv - Microbiology 2023Quote: ... or macroH2A-3Xflag (6 x 106 on 100 mm dish) were cross-linked with 1 % of methanol-free formaldehyde (Thermo Scientific, 28906) in DMEM for 10 min at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The pellets were resuspended in 4.5 mL distilled water to which is added 0.5 mL 6% hypochlorite solution (Fisher Scientific Cat# SS290-1) and gently rocked for 1 minute followed by centrifugation (800g for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 6×10 minutes in .05M PBS and incubated with both Alexa-Fluor 488 and 555 secondary antibody (1:1000; Thermo Fisher Scientific) in .05M PBS with .1% Triton x100 for 2 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 10 x 1 µL droplets of each diluted suspension were deposited on polystyrene material (6-well plates, Thermo Scientific, Cat. No. 140675) and exposed to ambient temperature and variable RH (20% ...
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bioRxiv - Cell Biology 2023Quote: ... was used to amplify the genes of interest according to the primers mentioned in the Table 1 from 15-20 ng of cDNA in the QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific) machine ...
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bioRxiv - Neuroscience 2023Quote: ... and placed in a 6-well cell culture plate filled with 1 ml/well of culture media containing 50% Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23% Earle’s Balanced Salt Solution ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2023Quote: ... 2 x 105 cells were seeded in a 6-well plate containing coverslips and transfected with 1 µg dsRNA mixed with Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2024Quote: MonoMac-6 effector cells were stained with 1 μM of CellTrace™ Far Red dye (CTFR, cat. no. C34564, Thermo Fisher Scientific) in PBS (cat ...
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bioRxiv - Genetics 2024Quote: ... RT-PCR products were fractionated by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (ThermoFisher Scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ v1.47 (NIH ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7 x 105 Huh7 cells were seeded in a 6-well plate and transfected for 16 h with 1 µg purified dsRNA mixed with Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio prior to actinomycin D chase.
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bioRxiv - Plant Biology 2024Quote: ... were used to amplify a 1:16 dilution of cDNA in triplicate in a Quantstudio™6 PRO real-time thermal cycler (ThermoFisher, Inc.) with a 61°C annealing temperature ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) and 1% (v/v) penicillin/streptomycin (P/S) (all from Thermo Fisher Scientific, Waltham, MA, USA). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was extracted from the amygdala of female offspring at P7 and P90 (n=6/mCHD, n=6/mHFD) using TRIzol Reagent (15596018; ThermoFisher Scientific, Ottawa, ON, CAD) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or 52- or 78-week-old C57BL/6 mice or C57BL/6-TgTM4SF5 mice by perfusion of the liver using collagenase type II (Life Technologies, Carlsbad, CA, USA) (Ateş et al ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were harvested from 6-week-old C57BL/6 mice and were differentiated into dendritic cells using supplemented culture medium: RPMI 1640 (Life Technologies, Waltham, Massachusetts, USA), 10% HIFBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...