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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Then sections were incubated in primary antibody solution (GFAP, 1:1000, Life technologies, 13-0300, Rat monoclonal antibody) overnight at 4 °C ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: ... Abmart) monoclonal antibodies followed by horseradish peroxidase (HRP)-conjugated goat-anti-mouse antibodies (1:5000 dilution, Thermo Fisher). Blots were developed in a Tanon Imaging System (Tanon 5200CE ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody was incubated overnight at 4°C [human Pax6 polyclonal antibody (1:100, Thermo Fisher Scientific)] ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA, Biolegend/ α-TAP, Thermo Fisher Scientfic) was added to the lysate and rotated for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with primary antibody (1/400 diluted rabbit anti-BACH2 antibody (PA5-23642, ThermoFisher Scientific) and 1/5’000 diluted rabbit-anti-GAPDH antibody (ab9485 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies included AlexaFluor488-conjugated and AlexaFluor594-conjugated secondary antibodies (goat anti-rabbit IgG, 1:500; ThermoFisher Scientific). After staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The secondary antibodies used were Alexa fluor 488-conjugated goat anti-rabbit antibody (A11008; Invitrogen; dilution 1:1000) and Alexa fluor 594-conjugated goat anti-mouse antibody (A11005 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Antibody staining was performed following in situ hybridisation with guinea pig anti-insulin antibody (Invitrogen, diluted 1:1000) and rabbit anti-glucagon antibody (ZYMED ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with primary antibodies and subsequently with the corresponding biotinylated secondary antibodies (1:1000 Goat anti-rabbit, Invitrogen). Signal amplification was performed using the ABC complex (Vectashield) ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 1% BSA in PBS with primary mouse anti-HuC/D antibody (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... the secondary antibodies donkey anti-chicken Alexa 488-conjugated antibody (1:1000, cat #: A78948 ThermoFisher Scientific Invitrogen, USA), donkey anti-mouse Alexa 488-conjugated antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... antibodies were incubated at 4°C overnight followed by secondary antibodies anti-rabbit Alexa 488 (Invitrogen, 1:500), anti-mouse Alexa 594 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cell were then immunostained with anti-primary antibodies used at 1:500 and then AlexaFluor conjugated antibodies (Thermofisher) used at 1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... the secondary antibodies donkey anti-chicken Alexa 488-conjugated antibody (1:1000, cat #: A78948 ThermoFisher Scientific Invitrogen, USA), donkey anti-mouse Alexa 488-conjugated antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-SYCP3 antibody (1:1,000) (24) follower by applying the Alexa fluorescence-labeled secondary antibodies (Thermo Fisher). PNA-Cy5 (Vector laboratories ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies and Alexa546-conjugated goat α-mouse and Alexa488-conjugated goat α-rabbit antibodies (ThermoFisher; 1:500). Images were captured on a Zeiss LSM-5 PASCAL laser scanning confocal microscope (KSU Division of Biology Microscopy Facility) ...
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bioRxiv - Cancer Biology 2023Quote: ... Alexa-conjugated goat-anti-mouse and anti-rabbit antibodies were used as secondary antibodies (1:1000, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The membrane was boiled for 30 min in water and incubated for 1 hour in 5% skim milk in TBS pH 7.4 then for 2 hours in primary antibody (1:2,000 ubiquitin monoclonal antibody 13-1600 Invitrogen) and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated with a secondary antibody solution containing fluorescent-labeled anti-mouse antibody (Thermo Fisher, 1:200), Streptavidin (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 1 hour incubation with primary antibodies and 20 min with AlexaFluor-conjugated secondary antibodies (Invitrogen) in 5% goat serum or 3% BSA in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Appropriate secondary antibodies were used in antibody dilution solution (1:500, goat anti-guinea pig Alexa 594, Invitrogen A11076 ...
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bioRxiv - Cancer Biology 2024Quote: ... the secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) or Alexa Fluor 488 Goat Anti-rabbit IgG (H/L ...
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bioRxiv - Cancer Biology 2024Quote: ... The secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) and Alexa Fluor 488 Goat Anti-mouse IgG (H/L ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with 1:750 secondary antibodies (Alexa Fluor 488-conjugated goat anti-rabbit antibody, A11008, Invitrogen) at 37 °C for 1.5 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked using 5% w/v non-fat dry milk in TBST for one hour at room temperature and then incubated with primary antibody (anti-Alix, 1:5000; Protein Tech, 12422-1-AP or anA-FloAllin-1, 1:5000; Fisher Scientific, BDB610820) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... the isolated RNA samples were reverse transcribed to cDNA using superscript II (Transcriptase inverse SuperScript™ II, Invitrogen™ Cat#18064022) following manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... Myosin II filaments are prepolymerized by adding monomeric myosin II (20 nM, chicken skeletal muscle, fluorescently labeled with Alexa 647, Life Technologies) to an actin free sample solution and allowing to incubate for 10 min.
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bioRxiv - Bioengineering 2021Quote: Viral RNA was extracted from 200μl of VTM using the MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit (Applied Biosystems) on the KingFisher™ Flex Purification System (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR product was cloned using Gateway BP Clonase II Enzyme mix and Gateway LR Clonase II Enyzme Mix (ThermoFisher Scientific) via donor vector pDonR211 into the final Doxycycline inducible expression vector pInducer20 [20] according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR fragment obtained was cloned into pCR Blunt II TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies) and sequenced.
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bioRxiv - Plant Biology 2019Quote: ... the pENTR/D PPC1 RNAi clone was recombined into intron-containing hairpin RNAi binary vector pK7GWIWG2(II) (Karimi et al., 2002) using LR Clonase II enzyme mix (Life Technologies). Agrobacterium-mediated stable transformation of K ...
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bioRxiv - Molecular Biology 2019Quote: ... the 3’ end of rpc37 was first amplified by PCR and cloned into pCR Blunt II-TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies). Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC ...
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bioRxiv - Biophysics 2020Quote: Madin Darby canine kidney II (MDCK-II) (ECACC, Cat. No. 00062107) and MDCK Myr-Palm-GFP H2B-mCherry cells were cultured in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... chloride hemipentahydrate (Cd2+), lead (II) nitrate (Pb2+), mercury (II) chloride (Hg2+), and hydrogen peroxide (H2O2, 30% in water) were purchased from Fisher Scientific. Buthionine sulfoximine (BSO) ...
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bioRxiv - Developmental Biology 2021Quote: ... 35 μm thick sections were used immunohistochemistry (IHC) of acetylcholine receptors (α-Bungarotoxin extracted from Bungarus muticinctus, conjugated to Alexa Fluor 647, Thermofisher #B35450) and nerve fibers (chicken anti-neurofilament-H antibody ...
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bioRxiv - Genomics 2020Quote: Plasmid p3xFLAG-CMV-14 carrying the complete sequence for progesterone receptor gene (HindIII924 - 938EcoRI) was introduced in Ishikawa cells using Lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer recommendations ...
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bioRxiv - Microbiology 2020Quote: ... SVECs lacking the IFN-α/β receptor (IFNAR1-CRISPR) were maintained in the same media as SVECs with 2 μg/mL puromycin (Gibco). Primary LECs (Cell Biologics ...
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bioRxiv - Microbiology 2021Quote: ... Vero-hSLAMF1 cells [52] stably express the human measles receptor SLAMF1 and were cultured in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific) containing 5% newborn calf serum (NCS ...
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bioRxiv - Cell Biology 2024Quote: ... The cell membrane of mESCs without HaloTag-receptors was fluorescently stained with wheat germ agglutinin Alexa Fluor 488 (Thermo Fisher Scientific, W11261) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with PBS and incubated with secondary antibody and primary conjugated α-smooth muscle actin antibody (1/1000 dilution, 1 hour, room temperature, A-11034; Molecular Probes, Eugene, USA. C6198; Sigma) before counterstaining with DAPI (5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa568- (1:1000) and Alexa647-coupled (1:500) antibodies were used (all from Thermo Fisher Scientific). For Western blotting ...
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bioRxiv - Neuroscience 2020Quote: ... Bassoon (1:400, SySy) followed by incubation with secondary antibodies (1:400, Alexa Fluor-conjugated, Invitrogen), counterstained with DAPI and mounted in Fluorsave (Calbiochem) ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight with antibodies directed to ZO-1 (61-7300, ThermoFisher Scientific, 1/100), E-cadherin (ab76055 ...
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bioRxiv - Biochemistry 2021Quote: ... goat anti-rabbit HRP conjugated antibody (WB 1:2000 or 1:5000, Thermo Fisher Scientific, 31460), goat anti-mouse HRP conjugated antibody (WB 1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... The Alexa 488 goat anti-rabbit secondary antibody (1:1500 in 1% NGS PBST, Life Technologies) was applied for one hr at RT℃ ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with secondary antibodies (anti-Mouse IgG 488, 1:1000 Invitrogen, #A11029 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with Alexa-labeled secondary antibodies for 1 h (1:500, Molecular Probes, USA). Sections were then washed in PBS and mounted in Fluoromount containing DAPI (Calbiochem ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were washed and incubated with secondary antibodies (Invitrogen catalog number in Table 1 – 1:200) for 30-60 minutes at room temperature in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... and secondary antibodies at room temperature for 1 hour (Alexa Fluor-conjugated, Thermo Fisher, 1:500). EdU staining was performed as previously (Mitchell- Dick et al ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated 1 hour in a 1:1000 dilution of Alexa Fluor 488-conjugated secondary antibody (ThermoFisher) in blocking solution ...