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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... using the Gateway LR Clonase II kit (Invitrogen). Integrity and orientation of inserts was checked further by Sanger sequencing before using plasmids to generate transgenic Drosophila.
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bioRxiv - Immunology 2023Quote: ... cDNA was reverse transcribed using SuperScript II (Invitrogen) and random primers (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... and reverse-transcribed with SuperScript II (Invitrogen, 18064022) using a barcode-specific primer (P7-pLSmp-assUMI-gfp ...
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bioRxiv - Microbiology 2023Quote: ... Platinum II Taq Hot start DNA-polymerase (Invitrogen) was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... SuperScript™ II Reverse Transcriptase (Invitrogen, 18064-14) was used with the following the protocol ...
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bioRxiv - Biochemistry 2023Quote: ... using the XCell II blot module (Thermo Fisher) at a constant current of 170 mA for 1 h at 4 °C ...
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bioRxiv - Immunology 2023Quote: Nunc Lab-Tek II chamber slides (ThermoFisher Scientific) or µ-Slide 8 Well Glass Bottom (IBIDI ...
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bioRxiv - Genomics 2023Quote: ... 0.5% (wt/vol) AlbuMAX II (Thermo Fisher Scientific), and 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... and Dispase II (Gibco 17105041; 3.5 mg/mL) in DPBS (Gibco 14190094) ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNA was generated using Superscript II (Invitrogen) from individual hearts as recommended by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... and digested with Collagenase II (ThermoFisher Scientific, 17101015). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... using the BP clonase II enzyme (#11789100, Invitrogen) to generate pDONR221-gCDC42attb ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNAs generated using SuperScript II (Life Technologies). Real-time PCR was performed on a Bio-Rad CFX Touch using SYBR Green PCR master mix (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... We used Gateway Clonase II (Invitrogen cat. 11791020) to insert the AXL cDNA (GeneCopoeia cat ...
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bioRxiv - Developmental Biology 2024Quote: ... After first-strand (SuperScript II, Thermo Fisher Scientific) and second strand synthesis (E ...
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bioRxiv - Molecular Biology 2024Quote: ... complemented with 0.5% (w/v) Albumax II (Invitrogen) at 4% hematocrit and grown at 37°C in a gas mixture 37 ...
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bioRxiv - Plant Biology 2024Quote: ... using Gateway BP Clonase II (Thermo Fisher Scientific) to generate pDONR P4-P1R MAP4K1p and pDONR P4-P1R MAP4K2p ...
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bioRxiv - Cell Biology 2024Quote: ... followed by cDNA synthesis using Superscript II (Invitrogen). The cDNA samples were subjected to qRT-PCR using the SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... 10 U/µL Superscript II (Thermo Fisher Scientific), and 1 µM of trP1-TSO] ...
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bioRxiv - Plant Biology 2024Quote: ... using Gateway LR Clonase II (Thermo Fisher Scientific). 1300 or 1928 bp upstream sequences of MAP4K1 or MAP4K2 ...
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bioRxiv - Molecular Biology 2024Quote: ... SuperScript II reverse transcriptase was purchased from Invitrogen. Marathon RT was purchased from Kerafast ...
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bioRxiv - Cell Biology 2020Quote: ... In brief 1 μg of total RNA was reverse-transcribed using 200 U reverse transcriptase Superscript II (Thermo Fisher Scientific) in the presence of 2.5 μM N6 random primers and 0.5 mM dNTP ...
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bioRxiv - Developmental Biology 2020Quote: 1 μg RNA was treated with DNase I and reverse transcribed to cDNA with Superscript II reverse transcriptase (ThermoFisher Scientific). The primers for real-time PCR were listed in S2 Table ...
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bioRxiv - Developmental Biology 2019Quote: ... then incubated with 10 ml of digestion medium (1 mg/ml of each Collagenase II and Dispase in HBSS, all from Gibco) for 30 min at 37 °C in a water bath ...
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bioRxiv - Bioengineering 2019Quote: ... or 1.5 × 105 cells ml−1 (cardiomyocytes) prepared using the Countess II FL Automated Cell Counter (AMQAF1000; Thermo Fisher Scientific) was introduced onto collagen hydrogels in 35-mm cell culture dishes without any reagent coating ...
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bioRxiv - Neuroscience 2019Quote: ... To perform PCR different primer sets were used (Tab.1) and PCR was executed with Phire Hot Start II DNA Polymerase (ThermoFisher).
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bioRxiv - Plant Biology 2020Quote: ... A total of 1 μg of DNA-free RNA was reverse transcribed using oligo(dT)18 primers and the SuperScript II enzyme (Invitrogen) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... 4 7 or 32 were amplified using specific primers (Supplementary table 1) and Gateway cloned into a pDONR221 entry vector using BP clonase II (Invitrogen) according to manufactures description ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The purified RNA samples were prepared for gel electrophoresis by adding 1 μl loading dye (gel loading buffer II, Invitrogen) followed by incubation at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: Total RNA (1 μg) was isolated from HEK293T cells and reverse transcribed into cDNA using random hexamers and SuperScript II RT (Invitrogen). The ACE2 and TMPRSS2 genes were amplified with GoTaq polymerase (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were resuspended in 1 × PBS with 0.04 % FBS and quantified and quality checked using a Countess II Automated Cell Counter (Thermo Fisher). A total of 1 ml of cells at ∼1200 cells μl-1 were submitted for sequencing.
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bioRxiv - Microbiology 2021Quote: ... 1×104 transfected cells were seeded into each well of 8-well Lab-Tek™ II chamber slides (ThermoFisher Scientific) for immunostaining analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... Culture media was replenished every 4 days and organoids were passaged by sequential digestion in 1 mg/mL Dispase II (Gibco) followed by TrypLE Express (Gibco ...
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bioRxiv - Genomics 2021Quote: Skin and wound samples were incubated in Dulbecco’s Modified Eagle Medium (DMEM) containing 5U/ml Dispase II and 1% Penicillin-Streptomycin (ThermoFisher Scientific) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2020Quote: ... We used a Nanoject II to inject 0.2 – 1 μl of 2% WGA-Alexa Fluor 555 (Thermo Fisher Scientific; #W32464) and 0.5% Fast Green (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... ileum from chickens were cut mechanically and incubated with RPMI 1640 medium with Collagenase II in a concentration of 1 mg/mL (Gibco) for 40 minutes at 37 °C with shaker ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were mixed with 100 μl of PEI (4:1 v/w PEI:DNA ratio) in 500 μl of pre-warmed OptiMEM II reduced serum medium (Gibco, 1985070), after 10-15 min incubation at RT ...
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bioRxiv - Microbiology 2022Quote: ... with 0.3 U µ L−1 Invitrogen PlatinumTM II Taq Hot-Start DNA Polymerase (Thermo Fisher ScientificTM Brazil, Lot 00813145), 1X PlatinumTM II PCR Buffer (Thermo Fisher ScientificTM Brazil ...
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bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl of DNase treated RNA was converted to cDNA using random hexamers (IDT) and SuperScript II Reverse Transcriptase (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cell pellets were then resuspended in 1 mL of flow buffer and cell counts were determined using a Countess II FL (Invitrogen). Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf ...
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bioRxiv - Molecular Biology 2023Quote: ... Single strand complementary DNA (cDNA) was generated from 1 µg RNA using dT-oligo primers and Superscript II reverse transcriptase (Invitrogen). PCR was performed using Phusion DNA polymerase (Finnzymes ...
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bioRxiv - Neuroscience 2023Quote: ... by RT-PCR (see methods below) and (ii) by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42 ...
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bioRxiv - Biochemistry 2024Quote: ... Cdc13-encoding baculovirus samples were generated by transfecting of 1 µg of bacmid DNA in 8 µL Cellfectin II reagent (Gibco) into 8×10 Sf9 cells in one well of a 6-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... the rabbit anti-TH antibody (1:2000) was detected using a goat anti-rabbit secondary antibody coupled to Alexa 546 (ThermoFisher Scientific, A-11010, 1:500) and eYFP was detected using detected using a chicken anti-GFP antibody (Aves Labs ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa-488-conjugated donkey anti-rabbit secondary antibody (Jackson, 711-547-003, 1/500) or Alexa-594-conjugated donkey anti-goat secondary antibody (Molecular Probes, A 11058, 1/500). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... neuronal bodies and projections were labelled with antibodies against MAP2 (Thermo Fisher, dilution 1:1000, secondary antibody: goat anti-rabbit Alexa Fluor 647, Life Technologies, dilution 1:1000) and Tuj1 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface CRT was detected using anti- CRT(N) or anti-CRT(Cmut) antibody (1:200 dilution) followed by staining with Alexa Fluor™ 647 conjugated secondary antibody (Invitrogen A21245, 1:2000 dilution). Total CRT in healthy donor platelets was stained with anti-CRT(N ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then washed three times followed by an incubation for 1 h with the following secondary antibodies: goat anti-rat Alexa Fluor 647 antibody (Thermo Fisher, #A78947, 1 µg/ml), goat anti-mouse Alexa Flour 488 antibody (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Lungs were inflated with an enzymatic digestion buffer (Dubecco’s modified Eagle’s medium [DMEM], 1 mg/mL collagenase type IV [Invitrogen], and 0.2 mg/mL DNase I [Sigma]), after which they were dissected away from the trachea and heart and incubated in 5 mL enzymatic digestion buffer in a 50 mL conical tube for 30 min at 37°C under agitation at 200 rpm ...