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Citations for Thermo Fisher :
2551 - 2600 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3x with 200μL of PBS containing 0.1% Tween-20 (PBS-T) and blocked with 200μLs of blocking solution (3% goat serum [Gibco, #16210072] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... After blocking with 10% Normal Goat Serum in PBS pH 7.4 with 0.1% sodium azide (Thermo Fisher Scientific) for 30 min (RT) ...
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bioRxiv - Cell Biology 2024Quote: ... was used for 1h blocking and cells were incubated with anti-myc tag primary antibody (MA1-21316, Invitrogen) diluted 1:500 for 2h in RT ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in secondary antibody diluted in blocking buffer [goat anti-mouse 488 1:4000 (ThermoFisher Scientific A11029)] for 30 minutes at room temperature with orbital shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then incubated overnight at 4 ºC in blocking buffer containing 10% Goat Serum (Thermo Fisher) and 0.01% Triton X-100 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for BrdU staining: 2 mL blocking buffer containing 20 μL BrdU monoclonal antibody (Invitrogen, B35128). Secondary antibody solution for BrdU staining ...
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bioRxiv - Bioengineering 2024Quote: ... and anti-acetylated tubulin staining: 4 mL blocking buffer containing 8 μL Alexa Fluor 488 (Invitrogen, A-11006), 8 μL Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... including 7992 sequences) appended with the iRT peptide sequence using Proteome Discoverer software (version 2.1, Thermo Scientific). The search parameters were set as follows ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were measured on an EASY-nLC 1200 ultra◻high◻pressure system (Thermo Fisher Scientific) coupled to a quadrupole Orbitrap mass spectrometer (Q Exactive HF and HFX and Exploris 480 ...
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bioRxiv - Systems Biology 2020Quote: Peptide extracts (1 μg on column) were separated on an Ultimate 3000 RSLC nano system (Thermo Scientific) using a PepMap C18 EASY-Spray LC column ...
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bioRxiv - Cell Biology 2020Quote: ... for peptide separation coupled online to a Q-Exactive Quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Waltham, Massachusetts) for MS/MS acquisition ...
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bioRxiv - Cell Biology 2020Quote: ... ~4 mg of peptides were enriched for phosphopeptides using a Fe-NTA phosphopeptide enrichment kit (Thermo Scientific) according to instructions provided by the manufacture and desalted ...
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bioRxiv - Developmental Biology 2021Quote: ... peptides were separated using revere phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The peptide sequences were verified by us using a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). HEK293 and Sf21 cells expressing FLAG-TRPR were first seeded onto poly-L-lysine-coated 96-well plates and cultured overnight ...
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bioRxiv - Neuroscience 2021Quote: 8 TMT reagents from a 10-plex reagent kit were used to label desalted peptides (Thermo Fisher) as directed by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluting peptides were on-line sprayed in a LTQ XL mass spectrometer (Thermo Scientific, Sacramento, US). Full scan mass spectra were collected in the linear ion trap in the mass range of m/z 350 to m/z 1800 Da and the 10 most intense precursor ions were selected for collision-induced fragmentation ...
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bioRxiv - Neuroscience 2021Quote: ... and an aqueous phase containing peptides was collected and dried with a SpeedVac (Thermo Fisher Scientific, U.S.A.).
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bioRxiv - Molecular Biology 2021Quote: Eluting peptides were analyzed in data-dependent acquisition mode on a Fusion Lumos mass spectrometer (ThermoFisher Scientific) coupled to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were purified using a 2-µl bed volume of Poros 50 R2 (Applied Biosystems) reversed-phase beads packed in Eppendorf gel-loading tips (Erdjument-Bromage et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The mass spectra were collected using the “Universal” method optimized for peptide analysis provided by Thermo Scientific. Full MS scans (375–1500 m/z range ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were analysed by nanoLC-MS/MS on a Q Exactive HF-X mass spectrometer (Thermo Scientific) as described previously with the following changes ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled samples were fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were analysed by nano-LC MS/MS using an Ultimate 3000 LC system (Dionex, Thermo Scientific) coupled to the triple TOF 5600 mass spectrometer (Sciex) ...
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bioRxiv - Immunology 2021Quote: ... Peptides were trapped on a pre-column (PepMap100 C18 3 μm; 75μm x 2cm, Thermo Fisher Scientific) and separated on an EASY-Spray column (ES803 ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides were loaded onto an Acclaim Pepmap 100 trap column (75 µm ID × 25 cm, Thermo Scientific) for 10 min at 3 µLs/min then eluted onto an Acclaim PepMap RSLC analytical column (75 µm ID × 25 cm ...
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bioRxiv - Microbiology 2019Quote: ... and eluting peptides were analyzed in a QExactive Plus hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Each sample was run in technical quadruplicates ...
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bioRxiv - Immunology 2019Quote: ... overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000; ThermoFisher Scientific, Waltham, USA). Then,15 μg of peptides were purified using ZipTipC18 pipette tips according to manufacturer’s instructions (Millipore ...
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bioRxiv - Bioengineering 2019Quote: Peptides were subsequently analyzed by ultra-high-performance liquid chromatography (UHPLC) using an Easy-nLC1200 (Thermo Scientific) online system coupled to a Q Exactive High Field mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Desalted peptides were eluted and directly analyzed by an Orbitrap Discovery mass spectrometer (Thermo Scientific, Waltham, MA). The HPLC system was extensively cleaned between samples ...
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bioRxiv - Biochemistry 2021Quote: Peptide mixtures were trapped on a 2 cm x 100 μm Pepmap C18 column (ThermoFisher Scientific, #164564) and separated on an in-house packed 50 cm x 75 μm capillary column with 1.9 μm Reprosil-Pur C18 beads (Dr ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were directly eluted into a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Data acquisition was performed in data-dependent and positive ion modes ...
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bioRxiv - Cancer Biology 2020Quote: ... LC-MS/MS analysis of the peptides was performed using a Q Exactive mass spectrometer (Thermo Scientific). The peptides were loaded onto a self-packed biphasic C18/SCX MudPIT column using a Helium-pressurized cell (pressure bomb) ...
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bioRxiv - Developmental Biology 2020Quote: ... Peptides were eluted from an Acclaim Pepmap 100 trap column (75 micron ID × 2 cm, Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 micron ID × 25 cm ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide analysis was carried out in a LTQ Velos nano-ESI linear ion trap instrument (Thermo Scientific). Protein identification was performed with the software PatternLab for Proteomics ...
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bioRxiv - Plant Biology 2020Quote: Peptides were analyzed using EASYnano-LC 1000 on a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific). Peptides were separated on a pre-column 75 µm × 2 cm ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Biochemistry 2020Quote: Peptides were analyzed by LC-nESI-MS/MS using an Orbitrap Lumos mass spectrometer (Thermo Fisher Scientific) fitted with nanoflow reversed-phase-HPLC (Ultimate 3000 RSLC ...
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bioRxiv - Cancer Biology 2020Quote: ... Tryptic peptides were analyzed using an EASY-nLC 1200 connected to an Orbitrap Fusion Lumos (Thermo Scientific) operated in a data-dependent manner ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were separated by reverse-phase chromatography (50 cm long EASY-Spray PepMap columns; Thermo Fisher Scientific) with a 140 min linear gradient of water/acetonitrile ...
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bioRxiv - Cell Biology 2021Quote: ... The digested peptides were labelled with 0.2 mg per reaction of 6-plex TMT reagents (ThermoFisher scientific) (126 ...
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bioRxiv - Cell Biology 2021Quote: Trypsinized peptides were separated via reverse-phase nano-HPLC using an RSLCnano Ultimate 3000 (Thermo Fisher Scientific). The mobile phase consisted of water + 0.1% formic acid as buffer A and acetonitrile + 0.1% formic acid as buffer B ...
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bioRxiv - Plant Biology 2021Quote: Peptide digests (2 μg) were separated by using a Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific) equipped with a reversed-phase Kinetex C18 2.6 μm column (100 X 2.1 mm ...
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bioRxiv - Biochemistry 2021Quote: ... Peptide ions were transferred to a hybrid quadrupoleorbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific, Bremen, Germany). Detailed MS instrument parameters are described in Supporting Information.
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Neuroscience 2021Quote: The peptides were analyzed using LC-MS (EASY-nLC™ 1000, Orbitrap Fusion EDT; Thermo Fisher Scientific). Injected peptides were trapped on an Acclaim™ PepMap™ 100 C18 LC column (3 µm particle size ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were loaded onto a C18 trap column (3 μm, 75 μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Microbiology 2020Quote: The digested peptides (200 ng) were analyzed by an EASY-nLC 1000 HPLC system (Thermo Fisher Scientific) interfaced with a Q Exactive™ Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... the peptide fractions from SCX were analyzed by LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) coupled with a customized LC system as previously described.15 The peptide fractions from high pH reversed-phase LC fractionation were analyzed an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting peptides were subjected to LC-MS/MS using EASY-nLC 1000 (Thermo Fisher Scientific, LC120) and Q Exactive (Thermo Fisher Scientific ...