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Citations for Thermo Fisher :
2551 - 2600 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2023Quote: Drosophila S2 cells were cultured at 23°C in Schneider’s media with 10% Gibco HI-FBS (Thermofisher) and 1% Gibco Antibiotic-Antimycotic (Thermofisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Microbiology 2024Quote: ... The entire pENTR effector library was then transferred into the pIB/V5-His vector (Invitrogen; Table S4) using LR Clonase II (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Developmental Biology 2023Quote: ... Su(H) coding sequence was cloned from GH10914 (DGRC Stock 5973) into pAc5.1/V5-His A (Invitrogen) using the following primers ...
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bioRxiv - Biochemistry 2023Quote: His-mATGL and its point mutants were recombinantly expressed in Expi293FTM cells (Thermo Fisher Scientific, Waltham, USA). The cells were cultivated in Expi Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... the crude DEL sample was loaded in Novex™ Hi-Density TBE Sample Buffer (5X, Invitrogen LC6678), and electrophoresis was run at 12 W constant power ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite software (v5.2.2 ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated for 1hour at 4 °C with His-Pure Cobalt Purification beads (Thermo Fisher Scientific). After incubation ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were grown in Ham’s F-12K medium (ATCC) with HI-FBS and 1x Antibiotic-Antimycotic (Gibco) at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Rubrum CbbM coding region in a His-bdSUMO plasmid acquired from Addgene65 using OneShotTM Top10 cells (Invitrogen). The constructs were expressed in OneShotTMBL21-AITM (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... with Radiant Probe Hi-ROX qPCR Kit (Alkali Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific). The expression of target genes was normalized to Gapdh expression.
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: HEK 293T (ATCC, Manassas VA, CRL-3216) cells were grown at 37°C with 5% CO2 in DMEM (Gibco, Waltham MA, #11965-092), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Genetics 2022Quote: Human embryonic kidney cell line (HEK 293T) was purchased from the American Type Culture Collection and cultured in DMEM medium (GIBCO, Cat. NO. 12430) supplemented with 10% FBS (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Equal ratios of heavy and light chain plasmids were co-transfected into HEK 293F cells using FreeStyle MAX (Thermo Fisher Scientific, catalog #16447100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2022Quote: ... Wild-type HEK293T and two independent clonal lines of CRISPR Serf2 KO mutant HEK 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; high glucose, pyruvate, 41966052), supplemented with 10 % Bovine Cow Serum (BCS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells were purchased from ATCC and cultured in high glucose DMEM with 2 mM GlutaMAX and pyruvate (Life Technologies catalog #10567022) supplemented with 10% heat-inactivated characterized fetal bovine serum (Hyclone ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...