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Citations for Thermo Fisher :
2451 - 2500 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... for human ACTB (Applied Biosystems, #Hs01060665_g1), and TGF-β1 (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... human IL-8 (Invitrogen™ 88808677), human IL-1beta (Invitrogen™ 88726177 ...
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bioRxiv - Immunology 2024Quote: ... goat anti-human IgG antibodies (Invitrogen) were added and incubated for 1Lh ...
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bioRxiv - Immunology 2024Quote: ... human IL-1beta (Invitrogen™ 88726177) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF (Gibco) + 25 ng/ml human FGF10 (Stemcell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... human serum albumin (Fisher Scientific, OSRHSA10), pooled human plasma (Innovative Research ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml human bFGF (Invitrogen), and 50 ng/ml recombinant human FGF-10 protein ...
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bioRxiv - Microbiology 2023Quote: ... Human Plasma-like Media (HPLM, GIBCO) was used as base media and supplemented with 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD326 (EpCAM) (#12932642, Invitrogen), and anti-human CD340 (HER2 ...
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bioRxiv - Immunology 2023Quote: Primary human fibroblasts (Thermo fisher; C0135C) were cultured in high glucose DMEM (Dulbecco’s modified media ...
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bioRxiv - Biophysics 2023Quote: ... 10 mg/ml human insulin (Gibco), 20 ng/ml EGF (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads human T-Activator (Thermo Fisher) anti-CD3/CD28 beads (at a 1:1 bead/cell ratio) ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F™ human cells (ThermoFisher Scientific) were grown in Expi293™ medium (Gibco ...
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bioRxiv - Genomics 2023Quote: Human Total Brain RNA (ThermoFisher Scientific) and a 48,502 bp double-stranded linear DNA from Bacteriophage Lambda (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... human CD45 (clone HI30; Thermo Fisher); mouse CD45 (clone I3/2.3 ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µg/mL human insulin (Gibco), and 1x pen/strep ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ42 human ultrasensitive ELISA Kit (Thermofisher), and D2R (Mybiosource) ...
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bioRxiv - Cell Biology 2023Quote: ... and human plasma fibronectin (Life Technologies) coated plastic culture plates or Ø 0.4 μm or Ø 3.0 μm pore Transwell inserts (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human astrocytes (Thermo Fisher, N7805100) were used for the co-culture conditions and grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... The GLOBINclear Human Kit (ThermoFisher Scientific) was then employed to deplete human globin mRNA in all samples ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human BMP4 (Thermo Fisher Scientific) at 10 ng/ml was added every third day in the presence of EGF and bFGF in the media ...
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bioRxiv - Cancer Biology 2024Quote: ... human (Thermo Fisher Scientific, cat# 4351379) using an Applied Biosystems™ QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... human embryonic kidney 293A cells (Invitrogen) were transfected with pHelper ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human FoxP3 EF450 (Invitrogen 48477642). Cells were stained with viability dye Zombie NIRtrade Fixable Viability Kit (Biolegend Inc ...
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bioRxiv - Microbiology 2024Quote: ... Heat stable recombinant human bFGF (Gibco) at 5U/ml was supplemented to organoid formation media only on day 0 ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies, Carlsbad, California, USA) was used according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293T/17 cells were purchased from ATCC (Cat. No. CRL-11268) were maintained in DMEM (Thermo Fisher Scientific, Cat. No. MT10013CV) with 10% fetal bovine serum and 1% Pen-Strep ...
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bioRxiv - Immunology 2020Quote: ... The purified plasmid DNA (both heavy and light chain) was transfected into a 30mL culture of HEK Expi293 cells (Thermo Scientific Expi293). Harvesting the culture after 5 days ...
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bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: The two lentiviral particles of the barcode and sgRNA vectors were individually transfected into HEK-293FT cells (Cyagen, no. HEKFT-30001) with lentivirus packaging plasmids using Lipofectamine 2000 (ThermoFisher, no. 11668027). To obtain the viral particles ...
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bioRxiv - Genomics 2022Quote: ... HEK-293T cells were grown on a 10 cm dish up to 70-80% confluency in HEK-media (KO-DMEM (Themro Fisher Scientific, 10829018), 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Systems Biology 2024Quote: ... VP4:VP2-complemented CVB3 A67G was prepared by transfecting HEK-293T cells with equal molar ratios of pcDNA CVB3 A67G and pcDNA3 VP4:VP2 using Lipofectamine 2000 (Thermo Fisher, 11668019). Viral supernatants were concentrated by ultracentrifugation (100,000 rcf for one hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: HEK-293T (CRL-11268) cells and PK-15cells (CCL-33) were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Carlsbad, CA USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... was placed at the beginning of the scFc sequence to facilitate protein secretion.54 The plasmid was transfected into HEK 293F cells (Thermo Fisher Scientific). After 88 h of incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in HEK-293T cells using either the Trans-Lentiviral ORF Packaging Kit (Dharmacon/Horizon Discovery) or Lipofectamine 3000 (Thermo Fisher Scientific). Viral supernatants were collected 72 hours (hrs ...
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bioRxiv - Microbiology 2023Quote: ... scrambled or PUS1/PUS7ko HEK-293Ts were transfected with 2 μg PAN and 2 μg ORF57-eGFP plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... in the presence of the selection antibiotic when required (HEK-293T-FLAG-CB1R; zeocin at 0.22 mg/mL, Thermo Fisher Scientific #R25001), and were periodically checked for the absence of mycoplasma contamination ...
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bioRxiv - Cell Biology 2022Quote: ... this mixture was added dropwise to confluent HEK-293T cells (ATCC, CRL-3216) in a T175 flask containing 35 mL DMEM media (Gibco #11965-092) and supplemented with 1mM sodium pyruvate (Gibco #11360-070) ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with psPAX2 (addgene plasmid #12260) and pMD2.G (addgene plasmid #12259) into HEK 293T cells using TurboFect (Thermo Fisher Scientific). Viral supernatant was collected on day 3 and 4 after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Wild type TGM2 and the TGM2-C277A mutant were overexpressed in HEK 293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in Dulbecco’s minimum essential medium (DMEM/F12; Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... Suspension Expi293 cells were grown to 2.5 million cell density and transiently transfected with ADAMs protease expression vectors using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific, # A14525). Medium was harvested after 6-7 days and protein was purified by Ni-NTA chromatography and assessed for quality and activity.
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...