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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Recombinant antibodies were generated using the Expi293 or Expi293 FUT8−/- system (ThermoFisher) using previously described protocols (60) ...
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bioRxiv - Neuroscience 2023Quote: ... while for the short we used the Taq DNA Polymerase Recombinant (Invitrogen) kit ...
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bioRxiv - Biophysics 2023Quote: Recombinant tubulin was purified by using Bac-to-Bac system (Life Technologies) as described previously18 ...
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bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were prepared using the Bac-to-Bac expression system (Invitrogen). The proteins were expressed in Trichoplusia ni (BTI-Tn5B1–4 ...
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bioRxiv - Molecular Biology 2023Quote: ... with the addition of RNaseOUT recombinant ribonuclease inhibitor (ThermoFisher Scientific 10777-019) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 0.5μL of 40U/μL of RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, #10777-019), and 0.5μL 200 U/μL SuperScript III reverse transcriptase ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng/mL recombinant RSPO1 (Thermo Fisher Scientific, cat# 120-38-500UG), and 100 ng/ml recombinant noggin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cholera Toxin Subunit B (Recombinant) Alexa Fluor 488TM Conjugate (C34775, Invitrogen, Belgium), Tetramethylrhodamine ...
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bioRxiv - Neuroscience 2021Quote: ... The presence of secreted Fc Fusion protein in the medium was confirmed by immunoblotting with goat anti-human IgG antibody (Invitrogen, A-21433, 1:500).
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bioRxiv - Immunology 2021Quote: ... was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific, Inc.) and labelled with Cy3 monoreactive dye pack (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated RNA was precipitated with chloroform/isopropanol and incubated with Dynabeads M-270 Streptavidin (Thermo Fisher) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated subunits of γ-TuRC were assessed by immunoblotting with Streptavidin-conjugated alkaline phosphatase (S921, ThermoFisher). For further conjugation of Alexa-568 dye to γ-TuRC ...
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bioRxiv - Cell Biology 2022Quote: Antibodies were biotinylated by overnight incubation at -4°C with EZ-link™ (Thermo Fisher Scientific,), at 20-fold molar excess ...
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bioRxiv - Genomics 2020Quote: ... The 5’ end cap structure was biotinylated and captured with streptavidin-coated magnetic beads (Thermo Fisher). After ligation of 5’ and 3’ adaptors ...
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bioRxiv - Immunology 2021Quote: ... Phage display was performed on biotinylated RBD immobilized on streptavidin magnetic beads (Dynabeads M-280, Invitrogen) or strep-tagged spike immobilized on Strep-Tactin XT magnetic beads (IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... PfAMA1 and PfMSP1 were biotinylated using EZ-link Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific) and HA proteins were biotinylated using AviTag technology (Avidity ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated in biotinylated donkey anti-goat secondary antibody (1:250, Thermofisher, A21432, USA) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: 5% biotinylated dextran-conjugated tetramethyl rhodamine 3000 MW (micro ruby, #D-7162; Molecular Probes, Eugene, OR) in distilled water47 ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was biotinylated using N-hydroxysuccinimide polyethylene glycol biotin (NHS-PEG4-biotin, ThermoFisher) by mixing 5.6 μM claudin-4 with 16.8 μM NHS-PEG4-biotin ...
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bioRxiv - Cell Biology 2021Quote: ... 100pmol biotinylated peptides (Peptide 2.0) were bound to 8μl streptavidin M-280 Dynabeads (Thermo Fisher Scientific) for 30mins in PBS at room temperature with agitation ...
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bioRxiv - Physiology 2021Quote: ... sections were incubated in 1:200 v/v biotinylated secondary antibody solution for 30 minutes (Invitrogen), rinsed in PBS and incubated in avidin-biotin-peroxidase complex (ABC Standard ...
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bioRxiv - Bioengineering 2021Quote: ... IgM or C1q samples were biotinylated using NHS-PEG4-biotin EZ-link kit (Thermo Fisher Scientific) and manufacturer conditions ...
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bioRxiv - Synthetic Biology 2021Quote: ... after which the clarified lysates containing biotinylated ERK2 and pERK2 were subjected to avidin agarose (ThermoFisher) to purify the Avi-tagged proteins according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... RBD-Fc was biotinylated with EZ‐link sulfo‐NHS‐LC‐biotin (Thermo Fisher Scientific, Waltham, MA) (RBD-Fc-Bio) ...
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bioRxiv - Microbiology 2020Quote: ... DNA hybridized to biotinylated-RNA baits was captured with streptavidin beads (Dynabeads MyOne Streptavidin T1, Invitrogen) and resuspended in 20µL water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA fragments hybridized to the biotinylated probes were recovered using MyOne Streptavidin C1 Dynabeads (Life Technologies). Third ...
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bioRxiv - Immunology 2022Quote: ... All fluorescently labeled antibodies and the biotinylated spike-trimer conjugated to streptavidin-allophycocyanin (SA-APC) (Invitrogen) were titrated before sorting ...
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bioRxiv - Immunology 2022Quote: ... Thioredoxin-fused peptides were chemically biotinylated with EZ-Link Sulfo-NHS-LC-biotin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and purified anti-IFNγ and BsAb were biotinylated by EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher) and buffer exchanged by Zeba Spin Desalting Columns (7K MWCO ...
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bioRxiv - Neuroscience 2021Quote: ... and with a biotinylated secondary antibody (goat anti-rabbit, Molecular Probes/Life Technologies, Grand Island, NY), before being reacted with DAB/peroxide ...
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bioRxiv - Neuroscience 2021Quote: ... and with a biotinylated secondary antibody (goat anti-rabbit, Molecular Probes/Life Technologies, Grand Island, NY), before being reacted with DAB/peroxide ...
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bioRxiv - Immunology 2022Quote: ... 5 μg biotinylated RBD was pre-coupled with 0.6 ul Streptavidin-AF647 (Invitrogen catalog no. S21374), used as RBD-647 ...
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bioRxiv - Genomics 2022Quote: ... 1:200 dilution of Anti-Mouse SSEA1 Biotinylated antibody (Thermo Fisher Scientific, Cat#: 13-8813-80), 1:200 dilution of Anti-Mouse Gr-1 APC-Cy7 antibody (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: ... The primary antibody was detected using a biotinylated goat anti-mouse IgG (QD209886, Life Technologies, USA), then an HRP-conjugated streptavidin (ab7403 ...
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bioRxiv - Genomics 2020Quote: ... the biotinylated RNA was resuspended in water and DNAse treatment was performed with TURBO DNAse (Invitrogen) following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The short biotinylated fragment cleaved following nicking was removed by binding to Streptavidin M270 Dynabeads (Invitrogen), and the remaining processed oligos were column-cleaned ...
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bioRxiv - Microbiology 2021Quote: ... cell surface sialic acid residues were oxidised and biotinylated using 1 mM sodium periodate (Thermo Scientific), 100 mM aminooxy-biotin (Biotium Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... Biotinylated PCR products were enriched with Dynabeads™MyOne™ Streptavidin C1 (Thermo Fisher Science, Australia). PCR products on Streptavidin C1 beads were washed with 400 μL 1 × B&W buffer (1 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 160 pmol biotinylated DNA adaptors were conjugated to 400 μg Dynabeads MyOne Streptavidin beads (Invitrogen, 65001) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Purified HPIV3 F was biotinylated using an EZ-link Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher) using a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Cell Biology 2021Quote: 400 pmol of biotinylated RNA was bound to 100 µl Dynabeads® M-280 Strepavidin (Invitrogen) in RNA-binding buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: hACE2 and the biotinylated RBDs were produced in 25 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated transcripts were produced from the linearized DNA in vitro using the MEGAscript kit (Life Technologies) and biotin-UTP (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated monoclonal antibodies were detected with APC-or Brilliant Violet 421-conjugated streptavidin (Thermo Fisher Scientific). For intracellular staining of Eomes ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Biochemistry 2024Quote: ... and biotinylated PDCoV SIL121_2014 ectodomain labeled with both PE and Alexa-Fluor 647 streptavidin (Life Technologies). Sorted IgG+ memory B cells were seeded at clonal dilution in 384-well plates an a monolayer of feeder mesenchymal cells in the presence of B cell survival factors ...
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bioRxiv - Genomics 2024Quote: ... Biotinylated RNAs were enriched with 30 µL of pre-washed Dynabeads MyOne Streptavidin C1 (Life technologies) in binding buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... hDPP4-Fc and G2 were biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Scientific) and 80% maximum binding concentrations against S-2P were determined with biotinylated proteins (hDPP4-Fc-biot and G2-biot) ...
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bioRxiv - Immunology 2024Quote: ... the biotinylated ODNs were detected with the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher, 89880) according to the manufacturer’s user guide.
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bioRxiv - Immunology 2023Quote: ... MIF-6xHis was biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit (Thermo Fisher Scientific), performed essentially as per manufacturer’s instructions ...