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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viruses were biotinylated by incubation in a 1 mg/mL Sulfo-NHS-LC-Biotin (ThermoFisher) for 3 hours at 37°C before being fixed and immunolabelled as described below.
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bioRxiv - Cell Biology 2020Quote: ... The rest of the sheared RNA was biotinylated with EZ-Link HPDP-Biotin (Thermo Fisher) in two biotinylation reactions per sample (300 μg RNA in 10 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... only biotinylated fragments containing the amplicons of interest are captured (following manufacturer’s instructions, ThermoFisher 60101). Dynabeads/DNA-complex is eluted in 23 µl H2O and used as template for the second PCR.
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bioRxiv - Biochemistry 2021Quote: ... NIH-CoVnb-112 was biotinylated using NHS-EZ-Link Sulfo-NHS-LC-Biotin (#21335, ThermoFisher) with a degree of labeling of 1 to reduce impact on binding ...
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bioRxiv - Genomics 2020Quote: ... and the biotinylated probes were detected using Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific, 89880) following the manufacturers instruction ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated and purified PCR1 products were then selectively captured using Dynabeads M-280 Streptavidin (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated EVs were loaded onto Zeba spin desalting columns (7 K MWCO; Thermo Fisher Scientific) to remove the remaining free biotin ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated RNA was purified using 80 μl MyOne Streptavidin C1 Dynabeads (Thermo Fisher Scientific #65002) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated mutant Cal09-HA (1.5μg) was complexed with streptavidin conjugated to APC (8μL; Thermofisher #S868) or PE (8μL ...
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was chemically biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Fisher) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Capture antibodies were biotinylated using EZ-Link™ NHS-LC-LC-Biotin (Thermo Fisher, #21343), and detection antibodies were conjugated using Sulfo-TAG NHS-Ester (MSD ...
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bioRxiv - Cell Biology 2022Quote: ... was carried out as follows: biotinylated peptides were incubated with streptavidin conjugated Dynabeads (65001, ThermoFisher) for 30 min RT followed by 2 h incubation with supernatant ...
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bioRxiv - Microbiology 2022Quote: ... Biotinylated RNA was bound 1:1 to Dynabeads My One Streptavidin T1 (Thermo Fisher Scientific) equilibrated in 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: The biotinylated baits were immobilized on 96-well streptavidin-coated plates (Thermo Fisher Scientific 15500). The wells were then washed three times with wash buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... 5FU was detected using an anti-mouse biotinylated secondary antibody (Thermo Fisher Scientific, Cat. # A10519) at 1:200 dilution in 1% BSA/PBS for 1 h at RT followed by incubation with streptavidin-conjugated 488 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... we prepared biotinylated OVA using EZ-link Sulfo-NHS-LC-Biotinylation Kit (Thermo Fisher Scientific), followed by dialysis in PBS for 24 hours to remove free biotin ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-PCDH15 8D1 capture antibody was biotinylated using NHS-PEG4-biotin (Thermo Fisher CAS# 21330) and anti-LHFPL5 antibody was labeled with Alexa647 using a Zip Alexa Fluor Rapid Antibody Labeling Kit (Thermo Fisher CAS#Z11235) ...
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bioRxiv - Biophysics 2024Quote: ... Biotinylated half-antibody fragments were conjugated with streptavidin-coated quantum dots QD655 and QD585 (Invitrogen). Before imaging ...
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bioRxiv - Immunology 2023Quote: ... The biotinylated PNA or E492 antibody were detected using streptavidin coupled to peroxidase (ThermoFisher Scientific) and the recombinant Clec4F was detected using a goat anti-human IgG coupled to peroxidase (AbCam ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated antiPD-1 antibody (clone J43) and isotype control (clone eBio199Arm) were purchased from Invitrogen.
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bioRxiv - Pathology 2023Quote: ... Biotinylated toxin was visualized using a SuperSignal West Pico PLUS Chemiluminescent Substrate kit (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Cel7A was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Scientific, catalog number: 21343), which labels the primary amines of exposed lysine residues ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated M2e-MAb was separated from non-reacted material by dialysis (10kd MWCO; ThermoFisher Scientific) in 1X phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: 5’ Biotinylated-dT32 was immobilized onto pre-equilibrated 20μl streptavidin coated magnetic beads (Thermo Scientific) at saturating concentrations before being washed with several 1ml volumes of wash buffer (50mM Tris-HCl ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated antibodies were purified using Zeba™ Spin Desalting Columns (7K MWCO, 0.5 mL, ThermoFisher). Recovered protein was quantified using Micro BCA™ Protein Assay Kit (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... capture antibodies were biotinylated using EZ-Link™ NHS-LC-LC-Biotin (Thermo Fisher, #21343), and detection antibodies were conjugated using Sulfo-TAG NHS-Ester (Meso Scale Diagnostics (MSD) ...
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bioRxiv - Biophysics 2023Quote: ... and EGF biotinylated at a 1:1 stoichiometry was purchased from Life Technologies (# E- 3477). Streptavidin-conjugated quantum dots (QD ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated fragmented nascent transcripts were enriched a total of three times using streptavidin Dynabeads (Invitrogen) and TRIzol LS reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... biotinylated probes were visualized using three layers of antibodies: streptavidin Alexa Fluor 750 conjugate (Invitrogen), biotinylated goat anti avidin (Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... biotinylated goat anti avidin (Vector) followed by a second streptavidin Alexa Fluor 750 conjugate (Invitrogen). Digoxigenin labeled probes were visualized using two layers of antibodies ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Fragments containing the biotinylated adapter were purified with Dynabeads™ M-280 streptavidin (Thermo Fisher). The end-repair reaction was done using the End-it DNA end-repair kit (Lucigen) ...
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bioRxiv - Neuroscience 2024Quote: ... Bound cytokine was detected using a biotinylated IL-6 detection antibody (ThermoFisher Scientific, MP5-32C11), streptavidin horseradish peroxidase (strep-HRP) ...
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bioRxiv - Genomics 2024Quote: ... 1 mM EDTA) containing 5 μg/ml biotinylated psoralen (EZ-Link Psoralen-PEG3-Biotin, ThermoFisher). Cell suspensions were incubated in the dark at 4 °C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... 5’-Biotinylated probes were hybridized to the membrane overnight at 42°C in ULTRAhyb (Ambion) hybridization buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... T0M20 antibodies (polyclonal, cat number PA5-52843) and Alexa 568-tagged secondary antibodies were from ThermoFisher. The imaging was performed using Nikon SP-1 microscope and analyzed using FIJI (Schindelin et al. ...
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bioRxiv - Cell Biology 2019Quote: Creation of the 3xmEGFP tagged RabE12 construct occurred by a two-element LR Gateway reaction (ThermoFisher) of entry clones pENT-L1-3xmEGFP-L5r and pENT-L5-RabE12-L2 ...
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bioRxiv - Neuroscience 2020Quote: ... N-terminally HA-tagged AMPAR constructs were expressed from the doxycycline inducible pcDNA4/TO vector (Invitrogen Cat ...
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bioRxiv - Biochemistry 2021Quote: Immunoprecipitation of HA tagged GAPDH (TDH3-HA) was performed using Pierce anti-HA beads (Thermo Fisher). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... 200 μl and the tagged Aβ(1-40) is incubated with Ulp1 SUMO-protease (either Invitrogen or produced recombinantly (Malakhov ...
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bioRxiv - Biophysics 2021Quote: ... they were transfected with EGFP tagged wt LA/A350P DNA along with Lipofectamine 2000 (Invitrogen, USA) in a ratio 1:1.5 ...
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bioRxiv - Bioengineering 2022Quote: MCF-7 Cells were fluorescently tagged with CellTracker™ Green CMTPX dye (Invitrogen™, Waltham, MA). A stock solution of 10 mM was prepared according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... In some cases 200-400 nl of fluorescent-tagged material (20% solution of FluoroEmerald Molecular Probes, Eugene ...
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bioRxiv - Immunology 2020Quote: ... The resultant FLAG-tagged STAT3 constructs were transfected into 293T using lipofectamine 3000 (Thermo Fisher Scientific). 48 hours after transfection cells were lysed (50mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... GST and GST-tagged SAMS were incubated with 40 μl GST magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C and then these beads were washed three times with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged gDNA was enriched using Dynabeads™ MyOne™ Streptavidin C1 (Thermo Fisher Scientific, Cat. 65001). The beads were allowed to room temperature while being resuspended on a HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and stained with Alexa Fluor 555-tagged donkey-α-goat secondary antibody (Molecular Probes, 1:1000) for 1 hour ...