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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Eukaryotic MV markers were detected by rabbit anti-TSG101 monoclonal antibody (1 μg/ml; Thermo Fisher; MA1-23296) and rabbit anti-ADAM10 monoclonal antibody (1 μg/ml ...
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bioRxiv - Genetics 2023Quote: ... The membrane was boiled for 30 min in water and incubated for 1 hour in 5% skim milk in TBS pH 7.4 then for 2 hours in primary antibody (1:2,000 ubiquitin monoclonal antibody 13-1600 Invitrogen) and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... the Pgk1 protein was recognized with a primary mouse monoclonal anti-Pgk1 (1:5,000; Thermo Fisher Scientific, 22C5D8), the aid tag was recognized with a primary mouse monoclonal anti-miniaid (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Microbiology 2024Quote: ... Both monoclonal antibodies were detected using a goat anti-mouse immunoglobulin HRP conjugate diluted 1:10,000 (ThermoFisher Scientific). Blots were then visualized using the Western Lightning Plus-ECL (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
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bioRxiv - Microbiology 2020Quote: ... cells expressing the surface CD4 receptor were enriched using the Dynabeads CD4 cell isolation kit (Life Technologies). They were seeded to develop single cell-derived clones ...
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bioRxiv - Microbiology 2021Quote: ... JCRB #1818) cells expressing the ACE2 or ACE and TMPRSS2 receptors respectively were cultured in DMEM (Gibco) supplemented with 5 % FBS (Fetal Bovine Serum) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with pCMV6 vectors bearing WT or mutant receptors using Lipofectamine 3000 transfection reagent (Invitrogen). Following 24 h culturing ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were pre-treated with Fc receptor binding inhibitor polyclonal antibody (eBioscience™, Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... N2a cells grown on coverslips were transferred to 35 mm culture plates containing 1 ml of serum and antibiotics free Opti-MEM (Gibco, ThermoFisher Scientific, Waltham, MA, USA). Then ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected with original ASAP1 or modified-ASAP (ASAP3β) using Lipofectamine 3000 (1 μg DNA, 2 μL P3000 reagent, 1.5 μL Lipofectamine for 35 mm dish; Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Bioengineering 2019Quote: ... and cryostat section (10μm) were stained using the following antibodies: rat anti-IL2 (eBioscience 14-7029-85) and anti-rat AlexaFluor488 (Invitrogen A21208). For vascular staining goat anti-CD31 (R&D AF3628 ...
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bioRxiv - Bioengineering 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8-μm cryostat sections of SKRC52 and CT26-CAIX stained with IL2-XE114-TNFmut and IL2-F8-TNFmut (final concentration 5μg/mL) and detected with rat anti-IL2 (eBioscience 14-7029-85) and anti-rat AlexaFluor488 (Invitrogen A21208). For vascular staining goat anti-CD31 (R&D AF3628 ...
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bioRxiv - Biophysics 2020Quote: Primary rat cortical neurons (RCN) prepared from the cortex of day-18 rat embryos (A10840-01, Life Technologies, Gaithersburg, MD) were suspended in a culture medium (21103-049 ...
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bioRxiv - Immunology 2021Quote: ... Macrophages were positively enriched from spleen single-cell suspensions using anti-F4/80 antibody (rat, Bioscience, Clone T45-2342)-coated Dynabeads (anti-rat IgG, Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor Goat anti-Rat 488 Invitrogen #A-11006, 1:1000 and Alexa Fluor Goat anti-Rabbit 594 Invitrogen #A-11012, 1:1000). Coverslips were mounted on slides with Fluoromount-G mounting media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the secondary antibodies (biotinylated goat anti-rat antibody, Jackson 112-066-072, 1/500; goat anti-rabbit Alexa fluor 635, Life technologies A31577, 1/500) and DAPI (4/1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... rat anti-Vasa (1:100, Developmental Studies Hybridoma Bank, RRID: AB_760351) and rabbit anti-GFP (1:2500, Thermo Fisher, cat# A-11122, RRID: AB_221569). Staining was detected with the following conjugated antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed by washing and cells stained for 1 hr at RT with Alexa Fluor-488 anti-rat secondary antibody (Thermo Scientific, A11006, 1:1000) for CD44 and Alexa Fluor-594 anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006; 1:500 and goat anti-rabbit conjugated to Alexa 647; ThermoFisher; A-21247; 1:500). After two final rinses in PB ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were then incubated overnight at 4 °C in rabbit anti-MYOC (1:50, custom) and rat anti-GFAP (Life Technologies, 130300, 1:500) in 5% NGS (MP Biomedicals ...
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bioRxiv - Immunology 2021Quote: ... followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific) [4] ...
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bioRxiv - Immunology 2022Quote: ... The 1A5 and 3F5 mAbs were conjugated with Alexa Fluor 647 (AF647) (Thermo Fisher Scientific) for flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... purified mAbs were buffer exchanged into PBS using Zeba™ Spin Desalting Plates (ThermoFisher Scientific) and stored at 4°C until use.
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bioRxiv - Immunology 2020Quote: ... purified mAbs were buffer-exchanged into PBS using Zeba Spin Desalting Plates (Thermo Fisher Scientific) and stored at 4°C until use.
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bioRxiv - Immunology 2023Quote: ... Biotinylated M2e-MAb was separated from non-reacted material by dialysis (10kd MWCO; ThermoFisher Scientific) in 1X phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rat 568 (Invitrogen, cat #A11077) antibodies in a dark humidified chamber for one hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-rat conjugated to Alexa647 (Thermofisher, A21472). Alexa568 conjugated phalloidin (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and anti-rat Alexa 488 (Invitrogen, A-11006) (1 μg/mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat 647 (#A-21247; Invitrogen). Nuclei were stained with DAPI (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... A goat α-rat Alexa488 secondary antibody (Invitrogen) was used at 1:1,000 in blocking solution and nuclei stained with DAPI.
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bioRxiv - Molecular Biology 2020Quote: ... rat α-mCherry (Thermo Fisher Scientific, M11217, RRID:AB_2536611); rabbit α-MPP8 (Proteintech ...
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bioRxiv - Immunology 2019Quote: ... goat anti-rat Alexa Fluor 568 (Molecular Probes). Sections were stained with DAPI and mounted with Prolong Gold (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rat 568 and anti-Human 647 (Invitrogen) were diluted 1:1000 in PBS-Triton X-100 0.1% with 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... Alexa Fluor 647-anti-Rat IgG (ThermoFisher Scientific). Reagents ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa488-conjugated anti-rat IgG (A-11006, Invitrogen), Alexa594-conjugated anti-rabbit IgG (A21207 ...
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bioRxiv - Genetics 2019Quote: ... Alexa Fluor 647 goat anti-rat (ThermoFisher, A21434), and Alexa Fluor 555 goat anti-rat (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and collagen1 rat tail were purchased from Gibco. All the chemicals were of analytical quality ...
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bioRxiv - Bioengineering 2020Quote: ... Goat anti-rat IgG Alexa FluorTM 488 (Invitrogen) was used as detection antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Rat IgG1 isotype control (Thermo Fisher MA1-90035) was used for IgG control ...
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bioRxiv - Cell Biology 2021Quote: ... goat anti-rat Alexa fluor 633 (Invitrogen, A21094), all used 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and donkey anti-rat 488 and 647 (Invitrogen, A-21208 and Jackson immunoresearch ...
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bioRxiv - Cell Biology 2019Quote: ... or Alexa Fluor 488 anti-rat (Molecular Probes)] both dilutedin Tyrode’s buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse CD34 (labelled with AF700) (ThermoFisher Scientific – 56-0341-82 ...
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bioRxiv - Cell Biology 2021Quote: ... coupled Dynabeads® Sheep Anti-Rat IgG (Invitrogen, 50 µl/ brain ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa 680 (goat-anti-rat, A21096, Molecular Probes) Alexa 680 (donkey-anti-rabbit ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibody CD117 (cKit) rat anti-mouse (Invitrogen) was combined with secondary antibody Alexa Fluor-488 goat anti-rat (Invitrogen ...