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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 6 Bromo 2 trifluoromethyl 3H imidazo 4 5 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 1.2 M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 µg/mL DAPI (4’, 6-diamidino2-phenylindole; Molecular Probes). Cells were imaged with a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Neuroscience 2024Quote: Total protein from whole cell lysates of mPFC and NAc (6-8 µg) were loaded onto NuPAGE 4-12% Bis-Tris gels (Invitrogen) under reduced and denatured conditions (Pierce 4X LDS sample buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells were infected with lentivirus for 4-6 hours before selection in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 4 EBs/well were transferred to an Ultra-low attachment 6-well plate (cat#: 07-200-601; Thermo Fisher Scientific) and maintained in Maturation Medium (STEMdiff™ Cerebral Organoid Basal Medium 2 + Supplement E + 100 µg/mL Primocin ...
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bioRxiv - Immunology 2019Quote: ... stained with Alexa Fluor™ 488 phalloidin for 3h at a dilution of 1/100 (Thermo Fisher Scientific, A12379) and DAPI at a dilution of 1/80000 for 10min ...
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bioRxiv - Biophysics 2019Quote: Peptide digests were dried by vacuum centrifugation and dissolved in 20 µl of 5% formic acid and injected at a flow rate of 5 μl/min onto an Acclaim PepMap 100 μm × 2 cm NanoViper 5-μm C18 trap (Thermo Scientific) using mobile phase A containing water and 0.1% formic acid ...
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bioRxiv - Synthetic Biology 2019Quote: ... × 5 mm 5 µm 100 Å and an Acclaim PepMap RSLC 75 µm × 25 cm 2 µm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... B-27 supplement and B-27 supplement minus antioxidants were purchased from Life Technologies (Grand Island, NY). Cocaine hydrochloride ...
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bioRxiv - Neuroscience 2023Quote: ... 0.2% GibcoTM Amphotericin B (250 µg/mL of Amphotericin B and 205 µg/mL sodium deoxycholate, Gibco) and 0.25% penicillin-streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... 5% CO2 and 37°C in neuronal culture medium (Gibco Neurobasal medium supplemented with 1x Gibco B-27 supplement, 2 mM L-glutamine and 250 μM penicillin-streptomycin; all Thermo Fisher Scientific). After 24 h ...
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bioRxiv - Microbiology 2020Quote: ... NSCs were differentiated into HMNs utilizing a neuronal differentiation medium prepared in Neurobasal Medium with B-27 Serum-Free supplement (2% [vol/vol]) (Thermo Fisher Scientific), and GlutaMAX-I (2 mM) ...
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bioRxiv - Neuroscience 2019Quote: ... Dissociated cells were filtered through 40 µM strainer and washed twice in Hibernate-A media with B-27 (2%, Thermo Fisher Scientific), GlutaMAX (0.5 mM) ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase was removed and the cells were then resuspended in DMEM with 2% B-27-supplemented 1% FBS and 1% PSF (Thermo Fisher Scientific) and plated on PDL/Laminin-2020-coated culture plates.
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bioRxiv - Systems Biology 2024Quote: ... cells were cultured for 72h with 10 nM SB431542 in culture media containing DMEM/F12 with 1x GlutaMAX/1x insulin-transferrin- selenium/1x NEAA/2% B-27 (Gibco 17504-044)/90 µM 2-Merceptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... using a 90 min gradient with a 250 nl/min flow rate of increasing Buffer B concentration (from 2% to 60%) on a High-Performance Liquid Chromatography (HPLC) system (Thermo Fisher Scientific), ionized with electrospray ionization (ESI ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then washed with PBS containing 0.1% Triton X-100 (PBST) 3 times and blocked for a minimum of 2 hr with Blockaid® (B-10710, ThermoFisher Scientific). IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution ...
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bioRxiv - Cell Biology 2023Quote: ... were cotransfected with pFRT-B-GRPA34 (Supplementary Table 2) (encoding GFP-RPA34) and pOG44 Flp-recombinase using Neon transfection (Cat.No.: MPK5000, Invitrogen, Waltham, Massachusetts, USA). Four hours after transfection the cell culture medium was exchanged and cells were grown for 48 h and selected with 2.5 mg ml-1 blasticidin (Cat.No. ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from day 2 LPS blasts seeded with sorted B cells using TRIzol reagent following the manufacturer’s instructions (Life Technologies, Carlsbad, CA). Genomic DNA was extracted from flow-purified naïve and GC B cells using lysis buffer (0.2% SDS ...
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bioRxiv - Immunology 2024Quote: ... using a 90 min gradient with a 250 nL/min flow rate of increasing Buffer B concentration (from 2% to 60%) on a High-Performance Liquid Chromatography (HPLC) system (Thermo Fisher Scientific) and ionized using an electrospray ionization (ESI ...
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bioRxiv - Molecular Biology 2024Quote: ... using a 90-minute gradient at a flow rate of 250 nL/min with increasing concentration of Buffer B (from 2 % to 60 %) on a high performance liquid chromatography (HPLC) system (Thermo Fisher Scientific), ionized with an electrospray ionization (ESI ...
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bioRxiv - Biophysics 2020Quote: ... 10,000 units/mL of penicillin, 10,000 mg/mL of streptomycin and 25 mg/mL amphotericin B, Gibco, Thermo Fisher Scientific, 5% horse serum) for up to 7 days at 16 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the left hemisphere VMH was pressure injected with 180 nl of a bi-directional neural tracer cocktail consisting of 5 μg/μl retrograde Cholera toxin subunit B conjugated with Alexa Fluor 488 (Life Technologies, cat# C22841) and 50 μg/μl anterograde dextran amine 10,000 MW conjugated with Alexa Fluor 488 (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Following thawing on ice the cells were resuspended in bacterial protein extraction reagent B-PER (5 ml/g fresh weight) (Thermo Scientific, Waltham, USA) supplemented with Protease Inhibitor Mix-B (Serva ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We used para-chlorophenylalanine (PCPA, a.k.a. DL-4 Chlorophenylalanine, fenclonine) (CAS: 7424-00-2, ThermoFischer, Acros Organics, Geel, Belgium; stored at 4°C), an inhibitor of the enzyme tryptophan hydroxylase (TPH) ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Developmental Biology 2020Quote: The retinas of juveniles (n = 6 retinas from 5 animals) and adults (n = 5 retinas from 4 animals) were dissected out and put in RNAlater (Ambion Inc., Austin, TX, USA). RNA extraction was performed using the RNeasy mini kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were intracardially perfused for 5 minutes with ice cold 1x DPBS followed by 5 minutes of ice cold 4% paraformaldehyde (PFA; Thermo Scientific, Fair Lawn, NJ). Following perfusion ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Neuroscience 2021Quote: NIH-3T3 cells were seeded on 6-well plates and allowed to grow to 70% confluency at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...