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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 6 Bromo 2 trifluoromethyl 3H imidazo 4 5 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)) was obtained from Fisher Scientific (Pittsburg, PA). Peptides were reconstituted at 25 mg ml-1 in nuclease-free ultra pure water as per the manufacturer’s instructions and stored as aliquots at -20°.HP1α was reconstituted (0.5 mg ml-1 ...
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bioRxiv - Immunology 2024Quote: ... for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher) to remove excess unbound biotin.
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher) to remove excess unbound biotin ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher, A42565) in fresh PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-4 μg of plasmid was mixed with 500 μL Opti-Mem (Gibco 31985070), after which 2.5 μL Plus Reagent and 8 μL Lipofectamine LTX (Invitrogen 15338100 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed in 4% formaldehyde containing 2 mg/mL DAPI nuclear stain (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... sperm were loaded with 2 mM of Fluo-4 AM (Invitrogen, Carlsbad, California, USA) diluted in 10% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... for 1 h at 4°C.The DynaMag™-2 magnetic rack (Thermo Fisher Scientific) was used for flow-through removal and washing ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mL lysates from each sample were electrophoresed on 4-12% NuPAGE gels (Invitrogen)38.
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bioRxiv - Cell Biology 2019Quote: ... Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670, 0.001% Brij35 ThermoFisher, #28316 ...
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bioRxiv - Microbiology 2021Quote: Cells were reverse-transfected (transfected while plating) in 6-well plates (3.5 -5 × 105 cells per well) using Lipofectamine RNAimax transfection reagent (Invitrogen), following standard protocols ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were plated in 6 well plates and transfected with 5 μl Lipofectamine RNAiMAX transfection reagent and siRNAs (Invitrogen) at 50 nM final concentration according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with transfection complexes for 5-6 hours and then replated onto #1.5 coverslips (Warner Instruments) coated with Collagen IV (Gibco). Cells were imaged in DMEM/10% FBS ...
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bioRxiv - Immunology 2020Quote: ... animals previously injected with vaccine or PBS where injected 24 hrs before sacrifice in the same footpad with 30 μL of 0.5 mM 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen). For assessing migration after 24 hrs ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2019Quote: ... To initiate transcription 4 µL of 5 X first strand buffer (Invitrogen, Cat No. 1889832) and 1 µL each of 0.1 M DTT (Invitrogen ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 23°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight (4° C) with 5 µg of Cx36 (Thermofisher, 37-4600, RRID: AB_2533320) or NMDAR1 (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ul of each sample was loaded on 4-12% Bis-Tris gels (Thermofisher).
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bioRxiv - Molecular Biology 2021Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside in DMSO (0.1-1mM, AICAR, Fisher Scientific), or isoproterenol (5-25µM ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min and loaded onto 4%–12% Nupage Bis-Tris gels (Novex/Life Technologies). Proteins were transferred using the iBlot2 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen) and transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days by treatment with StemPro Accutase (ThermoFisher, Cat#A1110501), stored in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the 11.5 μL RNA were supplemented with 4 μL 5× first strand buffer (ThermoFisher Scientific), 1 μL 10 μM reverse primer ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
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bioRxiv - Immunology 2022Quote: To analyze cell morphology 5×104 cells were resuspended in 100mL PBS and cytocentrifuged onto slides at 350g for 5 minutes (Thermo Scientific, Cytospin 4) before fixation in methanol for 15 minutes and staining with May-Grünwald’s (VWR Chemicals ...
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bioRxiv - Immunology 2019Quote: Skin samples were collected from the cheek of mice at the indicated time points with a 4- or 6-mm biopsy punch into cold RPMI 1640 medium (RPMI; Gibco) and minced into smaller pieces with surgical scissors ...
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bioRxiv - Plant Biology 2019Quote: ... The respective antibodies or IgG were coupled for 4-6 hour to Protein G Dynabeads (50µl, 10004D, Thermo Fisher Scientific) and subsequently incubated overnight with equal amounts of sonicated chromatin ...
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bioRxiv - Neuroscience 2021Quote: ... were harvested from male and female adult WT C57BL/6 mice and immersed in a 4% paraformaldehyde solution (PFA) made in phosphate-buffered saline (PBS, Cat #AAJ19943K2, ThermoFisher) and stored at 4 °C overnight prior to transfer to 15% sucrose solution in PBS for an additional 24 h ...
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bioRxiv - Microbiology 2020Quote: ... Organoids in expansion media grew in culture for 4-6 months and needed to be routinely passaged with TRYPLE (Gibco). To induce differentiation to a hepatocyte-like fate ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells in 6-well plates were transiently transfected with 4 µg of siRNA using Lipofectamine 2000 (Invitrogen). At 48h post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with 4 μg plasmid using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h culture ...
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bioRxiv - Microbiology 2019Quote: Total RNA was extracted from HIEs wells (in hW-CMGF+ or differentiation media for 4 days) or MA104 cells grown to confluency in a 6-well plate using TRIzol reagent (Ambion). Total RNA was treated with Turbo DNase I (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of SOSIP trimer or 3.2 μg of SOSIP-I53-50NP (which is equivalent to 2 μg of trimer) were loaded on a 4-12% Tris-Glycine gel or 6-18% Tris-Glycine gel (both from Invitrogen). For BN-PAGE ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and the supernatant centrifuged twice at 1000g for 10 min at 4°C (Sorvall RC-6 Plus Centrifuge, Thermo Scientific). The supernatant was centrifuged 8000g for 10 min at 4°C and then aspirated without disturbing the pellet ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged once a week (∼80% confluence/well) at 1:4-1:6 ratios using 1mg/ml collagenase (Gibco) to detach colonies and onto fresh ir-MEF plates.
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bioRxiv - Genomics 2022Quote: ... For each transfection 1.6 ug of plasmid DNA has been incubated for 20 minutes with 4 µl of Lipofectamine 2000 transfection reagent (Invitrogen) in 0.2 ml of OptiMEM media (Gibco ...