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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... arrays were incubated for 1h30 with AF647-conjugated goat anti-human IgG antibodies (at 1 µg/ml in PBS; Thermo Fisher Scientific), and revealed using GenePix 4000B microarray scanner (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Microbiology 2020Quote: ... They were then incubated with 1:200 diluted Alexa Fluor 594 conjugated goat anti-human IgG secondary antibody (Invitrogen, Thermo Fisher Scientific) for 45 min at RT and subjected to flow cytometric analysis with a CytoFLEX flow cytometer (Beckman) ...
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bioRxiv - Cell Biology 2019Quote: All human cells (listed in table 1) were cultured at 37°C in an atmosphere of 5% CO2 in DMEM (Thermo Fisher Scientific) supplemented with penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: The human monocytic cell line THP-1 (LGC, ATCC-TIB-202) was cultured and expanded in RPMI 1640 media (Gibco, 61870-010) supplemented with 10% FCS (BioConcept ...
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bioRxiv - Physiology 2023Quote: Immortalized human microvascular endothelial cells (HMEC-1) purchased from ATCC (Manassas, VA) were cultured in antibiotic-free MCDB131 (Life Technologies, Carlsbad, CA) supplemented with 10 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Bioengineering 2023Quote: ... To create SiRPα-Fc fusion soluble proteins the cDNA sequence of the full extracellular domain (aa 27-371) of human wild-type allele 1 SiRPα (wtSiRPα) cDNA (Uniprot: P78324) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1 ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times with PBS and labeled with secondary antibodies (goat anti-rabbit/human conjugated to Alexa Fluor 647; 1:1000; Invitrogen, #A21246/A21445) and FluoTag-X4 anti-GFP conjugated to Atto488 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Genetics 2023Quote: ... cells were stimulated with Dynabeads Human T-Activator αCD3/αCD28 at a bead-to-cell ratio of 1:2 (11131D, Thermo Fisher Scientific). Cell counts were determined every 2-3 days ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and a 1:1000 dilution of goat anti-human IgG (γ-chain specific) alkaline phosphate conjugate antibody (A3187, Thermo Fisher) was added and incubated for 1 h ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in 1 ml medium per well of a 24-well plate and stimulated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific), IL-7 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... one million human iPSCs were thawed and resuspended with 1 mL of Dulbecco’s Modified Eagle Medium F12 (DMEM F12) media (Thermofisher, Catalog No. 12634010) then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific, U.S.A.] with B27 Supplement [Thermo Fisher Scientific, U.S.A.]), then transferred to the hiPSC-derived cardiomyocytes for 48 hours of treatment either in the presence or absence of 100ng/mL of dexamethasone (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed three times with PBS and incubated with CD15 (C3D-1) mouse anti-human monoclonal antibody (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts, US], 10% FBS [PAA Cell Culture Company, UK], 1% GSP [Gibco, Massachusetts, US]). All cultures were maintained at 37 °C/5% CO2 in a humidified incubator ...
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bioRxiv - Neuroscience 2024Quote: ... Human slices were routinely stained with anti-NeuN (diluted 1:300 from stock; Rabbit anti-NeuN, Thermo Fisher Scientific PA5-37407, RRID:AB_2554049), visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Cancer Biology 2020Quote: ... E-cadherin and β-catenin antibodies followed by anti-rabbit antibody conjugated with Alexa-568 (Invitrogen) and anti-mouse goat antibody conjugated Alexa 488 ...
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bioRxiv - Cell Biology 2021Quote: The mouse β-cell line MIN6 (passages 18-30)22 was cultured in DMEM (Life Technologies) supplemented with 25 mmol/l glucose ...
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bioRxiv - Microbiology 2021Quote: The IFN-β levels in BALF were determined using mouse IFN beta ProQuantum Immunoassay kit (ThermoFisher). IFN-α and IFN-β levels in the supernatants of cultured BMDCs were determined by ELISA (PBL Biomedical Laboratories).
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bioRxiv - Synthetic Biology 2021Quote: ... streaked on LB/Ab agar plates supplemented with isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientific, catalogue) to a final concentration of 0.5 mM ...
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bioRxiv - Immunology 2019Quote: ... The IFN-α/IFN-β 2-Plex Mouse ProcartaPlexTM Luminex Panel kit used was from Invitrogen. Tetramethylrhodamine ...
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bioRxiv - Physiology 2020Quote: ... Samples were boiled for 10 minutes in 4% β-mercaptoethanol and 1x sample buffer (Thermo Scientific) and loaded on a 10% Bis/Tris gel with MES or MOPS running buffer for SDS-PAGE analysis ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from pseudoislets containing only β cells using an RNAqueous RNA isolation kit (Ambion). cDNA synthesis and quantitative reverse transcriptase PCR were performed as previously described2 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.05 and 0.1 μg) and pRSV-β-galactosidase (0.1 μg) using Lipofectamine and Plus reagent (Invitrogen). Luciferase and β-galactosidase activities were measured according to the manufacturer’s instruction (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and β-actin was quantified by RT-qPCR using Taqman primer and probes (Thermo Fisher Scientific). Expression levels of BMP receptors were reported relative to GAPDH.
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bioRxiv - Molecular Biology 2021Quote: ... β-Actin DNA was PCR amplified using a premade TaqMan probe/primer set (ThermoFisher, Cat #431182), while β-Actin cDNA in RNA analyses was quantified using a separate Taqman probe/primer set that amplifies across a splice junction (FP ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% fetal bovine serum and 0.05 mM β-mercaptoethanol (Fisher scientific, Cat. No. BP176-100). K562 cell was maintained in RPMI-1640 media with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: YFP-NLS-β-Actin expression constructs were transfected into HEK293TN cells using lipofectamine 2000 (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... total lysates proteins were diluted with PBS and LDS sample buffer—10% β-mercaptoethanol (Invitrogen; NP0007), separated on a 4 to 12% Bis–Tris polyacrylamide gel (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... expression of toxin was induced by the addition of isopropyl β-D-thiogalactopyranoside (IPTG, Fisher Scientific) to a final concentration of 1 mM ...
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bioRxiv - Genomics 2023Quote: ... for 2 h and with 33 nM C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside; ThermoFisher D2893) for 1 h ...
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bioRxiv - Immunology 2023Quote: The INS KO ECN90 β-cell line was generated by transfection with Lipofectamine CRISPRMAX (Invitrogen, #CMAX00001) with Alt-R S.p ...
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bioRxiv - Microbiology 2024Quote: ... Expression of toxins was induced by the addition of isopropyl β-D-thiogalactopyranoside (IPTG, Fisher Scientific) to a final concentration of 1 mM when the OD600 of the culture reached 0.5-0.6 after which the cultures were allowed to grow overnight ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...