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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml for TNF-α assessment in myeloid cells or Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
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bioRxiv - Biochemistry 2019Quote: For mammalian expression a DNA polynucleotide encoding the transmembrane domain (residues 1-295) of GtACR1 optimized for human codon usage was cloned into the pcDNA3.1 vector (Life Technologies, Grand Island, NY) in frame with an EYFP tag ...
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bioRxiv - Cell Biology 2019Quote: Human RBCs (1% in PBS) were double labeled with the lipidic dye R18 and the content marker calcein-AM (Molecular Probes, Life technologies). 20 µl of R18 (1 mg/ml in ethanol ...
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bioRxiv - Immunology 2021Quote: ... 32,33 were maintained by serial 2-day passaging in human foreskin fibroblast (HFF-1, ATCC® SCRC-1041™, American Tissue Culture Collection) monolayers cultured in DMEM (Thermofisher scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... T cells were activated through the T cell receptor subunit CD3 and the co-stimulator CD28 by the addition of Human T-Activator CD3/CD28 Dynabeads at a 1:2 bead:cell ratio (Life Technologies, cat. no. 111.31D) for 72 hours.
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Molecular Biology 2022Quote: Human adult epidermal keratinocytes were cultured in EpiLife medium supplemented with 10% Human Keratinocyte Growth Supplement (HKGS) and 1% Penicillin and Streptomycin at 37℃ in 5% CO2 (all from ThermoFisher Scientific, Waltham, MA). Calcium concentration was increased to 1.5 mM in the medium to induce cell differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... Internalization of Alexa Fluor 488-conjugated human α-syn PFFs (1 μM) and epidermal growth factor (EGF; 4 μg/mL; Thermo Fisher Scientific) were assessed similarly ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Biophysics 2023Quote: The nucleotide sequence encoding the human ADORA2A (1-316 aa) (UniProt ID C9JQD8) was codon optimized for Leishmania tarentolae expression by the GeneOptimizer software (Thermo Fisher Scientific, USA) and synthesized de novo (Eurofins ...
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bioRxiv - Immunology 2023Quote: ... or VHHASC with a C-terminal HA tag or C-terminal EGFP fusion under the control of the human elongation factor-1 α promoter (pEF1α) were generated using lentiviral vectors constructed by Gateway cloning (Thermo Fisher Scientific) using vectors modified from pRLL (a kind gift of Susan Lindquist ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no. Hs00427620_m1). Custom primers and probes were ordered from Genscript to quantify the different splice variants (see Table S1 for sequences and Figure 2 for alignment on the minigene sequence) ...
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bioRxiv - Cell Biology 2024Quote: ... The hTERT RPE-1 human epithelial cell line (ATCC-CRL4000) was obtained from ATCC and grown in DMEM/F12 (ThermoFisher Scientific, Carlsbad, CA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed once with Hanks’ Balanced Salt Solution (HBSS) and incubated with 1 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) diluted in HBSS/1% BSA for 1 hour at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide arrays were washed 3 times for 5 min at RT with TBST and incubated with the antibody Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher, 1/1000) diluted in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Immunology 2024Quote: ... Goat anti-human IgG conjugated with horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:2000 (Life technologies, Ref H10307) in PBS-T-milk for 1 hour at RT ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with DAPI (Thermo) and FluoZin-3 AM (Invitrogen; to detect zinc-enriched β-cells), and resuspended in phenol red-free media ...
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bioRxiv - Genomics 2019Quote: ... Proteins (20µg) were heated at 95°C with β-mercaptoethanol and then separated by SDS PAGE (Invitrogen). Nitrocellulose membranes were blocked in 5% nonfat milk in PBST for 1 hr and then incubated in primary antibody overnight at 4ºC ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.002 mM naphthalene solution was used as internal control and pure β-carotene (Thermo Fisher, Waltham, MA) at varying concentrations (mg/mL ...
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bioRxiv - Immunology 2021Quote: ... SA-β-galactosidase activity was measured using the CellEvent Senescence Green Flow Cytometry Assay Kit (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... FACS-Gal analysis was performed using warm hypotonic loading of fluorescein di β-D-galactopyranoside (Molecular Probes) on single cells as described 52 followed by immunophenotyping using relevant surface antigens as defined in Table S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhancer activity was assessed using the Dual-Light™ Luciferase & β-Galactosidase Reporter System (ThermoFisher Scientific, T1003) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Substrates to measure enzyme activity were 4-methylumbelliferyl β-gal (Acros Organics, Cat. No. 337210010, Waltham, MA), 4-methylumbelliferyl a-D-manopyranoside (Toronto Research Chemicals ...
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bioRxiv - Microbiology 2021Quote: Enriched β-GD (40 mg) was reduced with sodium borodeuteride (20 mg/ml) (ACROS Organics, cat.no. 194950050) in 1 M ammonium hydroxide for 90 minutes at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Monoclonal β-Actin (AM4302) antibody and EpiJET Bisulfite Conversion Kit were purchased from Thermo Scientific (PA, USA). Monoclonal CSTA antibody (ab10442 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 mM β-mercaptoethanol and 10 ng/ml basic fibroblast growth factor (bFGF) (all from Life Technologies). Medium was changed every other day until clonal iPSC colonies were manually picked and adapted to feeder-free culture conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was incubated with primary antibodies (rabbit anti-C19ORF66, Invitrogen # PA5-59815; mouse anti-β-Actin) diluted (1:2000 ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 U/ml and 50 μg/ml penicillin-streptomycin and 0.1 mM β-mercaptoethanol (all ThermoFisher Scientific), supplemented either with 2 μM Gö6983 (Tocris) ...
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bioRxiv - Microbiology 2022Quote: ... Fusion was arrested by adding 100 µL β-galactosidase substrate from the Gal-Screen reporter system (Invitrogen). The mixture was incubated at 37°C in the dark for 1 h before recording luminescence using a Tecan Infinite M1000.
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Bioengineering 2023Quote: Lysyl oxidase (LOX) mediated crosslink formation was inhibited via the treatment of β-aminopropionitrile (BAPN, Thermo Scientific) to the chorioallantoic membrane ...
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bioRxiv - Molecular Biology 2023Quote: Trabectedin used in survival and COMET experiments was from Tecoland and in GLOE-seq experiments from Lucerna-Chem AG. Illudin S was from MGI Pharma. β-actin (catalog no. MA5-15739) was from Invitrogen. XPG (catalog no ...
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bioRxiv - Immunology 2023Quote: ... in complete RPMI (RPMI 1640, 10% heat-inactivated FBS, 0.05mM β-mercaptoethanol and 1X Penicillin/Streptomycin Gibco) soluble αCD28 (Ultra LEAF© purified ...
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bioRxiv - Microbiology 2023Quote: ... each well was washed with phosphate-buffered saline (PBS) and β-lactamase loading solution (Invitrogen, Cat# K1030) [CCF4 substrate ...
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bioRxiv - Neuroscience 2023Quote: ... 1.5 μl of cholera toxin β subunit (CTB) conjugated with fluorescence Alexa-594 (2 μg/μl, Invitrogen) was injected into the vitreous 2 days before sacrificing the animals ...
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bioRxiv - Molecular Biology 2024Quote: ... Enhancer activity was assessed using the Dual-Light™ Luciferase & β-Galactosidase Reporter System (ThermoFisher Scientific, T1003) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... For flow cytometry detection of β-gal the CellEvent Senescence Green Flow Cytometry Assay Kit (C10840, Invitrogen) was used according to the recommended protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...