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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: iPSC lines were plated onto glutaraldehyde-activated glass coverslips coated with ESC-grade reduced growth factor basement membrane matrix (Geltrex, Gibco). Neuronal cultures were plated onto glutaraldehyde-activated glass coverslips coated with appropriate substrates as mentioned above ...
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bioRxiv - Developmental Biology 2024Quote: ... and bone marrow derived iPSCs (ACS-5003, ATCC) were cultured on ESC-grade reduced growth factor basement membrane matrix (Geltrex, Gibco) in NSC maintenance medium (Xcell Science ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the spike protein was detected using anti-SARS-CoV Spike Protein (SDelta3) monoclonal antibody (7G12, Thermo Fisher Scientific, CA, USA)) raised against immunogen aa168-461 of the S1 domain.
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bioRxiv - Neuroscience 2021Quote: ... and subjected to polyadenylating reaction using Escherichia coli poly(A) polymerase (E-PAP) Poly(A) Tailing Kit (Thermo Fisher Scientific, AM1350) in buffer containing 0.1mM ATP ...
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bioRxiv - Biochemistry 2022Quote: ... The constructs were transformed into competent DH5α (E. coli) cells and plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Scientific) to verify sequence integrity by sequencing (Eurofins Genomics) ...
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bioRxiv - Cell Biology 2022Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 12 μl 10% 2-butanone (Acros Organics) in ethanol streaked across the lid with a pipette tip for 1 h ...
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bioRxiv - Microbiology 2023Quote: 50ng of each plasmid containing viral cDNA genome were used for transformation of competent bacteria (E. coli strain DH5α, Thermo Scientific) by heat-shock ...
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bioRxiv - Neuroscience 2023Quote: ... The template was degraded by DpnI digest and circular PCR product was transformed into OneShot TOP10 chemically competent E coli (Invitrogen C4040). Both CMV:dreammist- GFPpA and CMV:dreammistA22W-GFPpA constructs were checked by Sanger sequencing.
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bioRxiv - Genetics 2023Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 18 mL 10% 2-butanone (Acros Organics) dissolved in ethanol on the lid ...
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bioRxiv - Cell Biology 2023Quote: ... strain was maintained on standard nematode growth media (NGM) plates seeded with OP50 E. coli bacteria (at 20°C. LysoSensor Green DND-189 (Thermo Fisher) was diluted in M9 buffer and added to 6 cm standard NGM plates (containing 12 mL of agar ...
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bioRxiv - Cell Biology 2022Quote: For extraction of membrane and cytosol proteins the cells were lysed according to the Mem-PER Plus Membrane Protein Kit protocol (#89842, ThermoFisher Scientific). The supernatants were kept frozen in −80 °C until total protein quantification ...
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived NGN2 neurons were maintained in 6-well plates and were lysed on day 21-24 for membrane protein extraction using the Mem-PER Plus Membrane Protein Extraction kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... All proteins were expressed in Escherichia coli one-shoot BL21star (DE3) cells (Invitrogen), as previously described (Selyutina et al. ...
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bioRxiv - Biophysics 2022Quote: ... These proteins were expressed in BL21 Star (DE3) Escherichia coli cells (Life Technologies). The expression and purifications were either in LB or M9 minimal media supplemented with 15NH4Cl with slight modifications as previously described (Conicella et al. ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3)pLysS (Thermo Fisher Scientific). 1-4 liters of cells were grown in Luria-Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: ... Matrix Science) against the Caenorhabditis elegans NCBI refseq protein database in the Proteome Discoverer (PD 2.1, Thermo Fisher) interface ...
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bioRxiv - Genetics 2021Quote: ... Membranes were imaged using MicroChemi (DNR Bio-Imaging Systems) and E-Gel Imager GelCapture Acquisition System (ThermoFisher).
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bioRxiv - Developmental Biology 2021Quote: ... on tissue cell culture plates pre-coated with 1:100 dilution of Geltrex Basement Membrane Matrix in DMEM/F-12 (both from ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Cells were grown as monolayers on geltrex-coated surfaces (Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, Gibco, A1413201) in NPC expansion medium ...
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bioRxiv - Cell Biology 2019Quote: ... hiPSC-CMs were reprogrammed from human cardiac fibroblasts as described previously (28) and maintained on Matrigel Membrane Matrix (Thermo Fisher Scientific) in mTeSR™ medium (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... were purchased from EBISC (European Bank for Induced pluripotent Stem Cells) and maintained on Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) in Essential 8™ Flex Media Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Invasion assays were carried out on either inserts coated with 75 μl Matrigel Basement Membrane Matrix (Fisher Scientific Uk Ltd, #11573620) diluted 1:1 with serum-free EVT medium ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were grown also on plates coated Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... Individual strips with a small amount of medium were then carefully moved to pre-chilled micro-centrifuge tubes and then mixed with cold reduced growth factor basement membrane matrix Geltrex (Thermo Fisher). The dilution of Geltrex by the introduced medium was less than 20% ...
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bioRxiv - Cancer Biology 2024Quote: ... with 50,000 mouse endothelial cells (harvested from mouse lungs by CD31 selection and expanded in vitro as previously described 67) and resuspended in 50 µl of Geltrex™ reduced growth factor basement membrane matrix (A1413301, Gibco). 100 µl of 1:1 GFP+:endothelial cell mixture was plated on transwell inserts with 0.4 µm pores and allowed to solidify for 30 mins in a humidified CO2 incubator (EOE ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (Thermo Fisher Scientific; catalog # PA5-114451).
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and cytosolic and membrane proteins were extracted with the use of a Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842).
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bioRxiv - Microbiology 2022Quote: The Spike gene sequence from the severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) was codon optimized (GeneArt, ThermoFisher) and gene blocks with overlapping sequences were synthesized by Bio Basic Inc (Markham ...
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and seeded on Geltrex matrix (Gibco) at 50% confluence in E8 medium ...
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bioRxiv - Microbiology 2020Quote: ... stored in matrix vials (Thermo Fisher) at a stock concentration of 30 mM or 10 mM in 100% Dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2023Quote: ... matrix was removed using dispase (Invitrogen) and organoids were transferred to 8-well IBIDI imaging slide ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were blotted on nitrocellulose membrane (Fisher scientific). The membrane was blocked in 5% milk/TBS-T for 1 h followed by overnight incubation with primary antibody at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to a nitrocellulose membrane (ThermoFisher, LC2000) and then blocked with Odyssey Blocking Buffer (Li-Cor Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (iBlot; Invitrogen). Membranes were blocked with TBST containing 3% BSA and incubated with antibodies against IRF1 (H-8 ...
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bioRxiv - Genetics 2021Quote: ... Separated proteins were transferred to PVDF membranes (Thermo Scientific) by electroblotting ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were electro-transferred to nitrocellulose membranes (Invitrogen) for 1 h at 100 V ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were blotted on a nitrocellulose membrane (Thermo Scientific). The following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were transferred to PVDF membranes (Invitrogen, catalog# IB24002) and immunoblotted with antibodies ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to 0.45 um PVDF membranes (ThermoFisher) using a mini blot module (ThermoFisher ...
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bioRxiv - Pathology 2021Quote: ... Proteins were transferred onto a Nitrocellulose membrane (ThermoFisher Scientific) using the iBlot2 dry blotting system (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to PVDF membranes via iBlot2 (ThermoFisher). Immunoblots were probed with polyclonal rabbit anti-PLA2G7 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein binds were transferred to PVDF membrane (88520, Thermofisher), which were blocked in Tris buffer (PH7.0 ...
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bioRxiv - Biochemistry 2022Quote: Proteins were transferred to a PVDF membrane (Thermofisher Scientific) following electrophoresis using Bolt 4-12% Bis-Tris Plus gels (Thermofisher Scientific).