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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and SARS-CoV S protein were confirmed by transfection with the Turbofect transfection reagent (ThermoFisher) followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Biochemistry 2023Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Neuroscience 2019Quote: ... Membrane proteins were extracted using the Mem-PER Plus Membrane Protein Extraction Kit (Cat# 89842; ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions using a mixture of protease and phosphatase inhibitors (10 µl/ml ...
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bioRxiv - Cell Biology 2019Quote: ... ozone-activated via corona arc-discharge (30s) and coated with reduced growth factor basement membrane matrix (Geltrex®, Gibco) for 1h at 37°C to provide attachment sites similar to the cardiac basement membrane ...
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bioRxiv - Genetics 2020Quote: ... on tissue culture dishes coated with Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (A1413202, Gibco™) and maintained in 5% CO2 incubators at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... on tissue culture dishes coated with Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Gibco™, A1413202) and maintained in 5% CO2 incubators at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... R&D systems) and Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix (Matrigel) (Fisher Scientific, #356231) at a 30/70 ratio ...
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bioRxiv - Biophysics 2021Quote: ... the protein construct was transformed into the Escherichia coli BL21 (DE3 Invitrogen) competent cells ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting proteins were expressed in Escherichia coli BL21(DE3) cells (Invitrogen) after induction by 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 18°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein was expressed in Escherichia coli BL21(DE3) cells (Life Technologies) using a pET-46 tobacco etch virus (TEV ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Escherichia coli BL21(DE3) pLysS (Invitrogen) transformed with respective plasmids ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Microbiology 2019Quote: ... and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (Avanti Polar Lipid). LTA (Lipoteichoic acid from S. aureus) and LPS (lipopolysaccharide from E. coli) (both obtained from Invitrogen) were also tested ...
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bioRxiv - Cell Biology 2023Quote: ... differentiated monocytes were preincubated for 30 mins with PBS or 25 μμM Cytochalasin D and then incubated with pHrodo red E coli bioparticles (Thermofisher) with or without 25 μM cytochalasin D for 1 h 30 mins ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Microbiology 2020Quote: ... RBD from SARS-CoV and SARS-CoV-2 were biotinylated (Thermo Fisher) and immobilized on Streptavidin (SA ...
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bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
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bioRxiv - Immunology 2022Quote: ... The membrane proteins of KCs or ECs were enriched using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific) with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Membrane proteins were extracted from cell pellets using the Mem-PER™ Plus Membrane Protein Extraction Kit (89842, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... and the cytosolic and membrane proteins were separated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher: 89842) with cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2021Quote: ... was added to the Lysing Matrix E tube and supplemented with 6 µL of lysozyme (50 mg mL-1, Thermo Fisher Scientific, Cat. no. 90082), followed by a 60 min incubation at 37°C with mixing at 300 rpm ...
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bioRxiv - Physiology 2020Quote: ... Proteins were blotted onto nitrocellulose membranes (Thermo scientific) and blocked with 25 ml of 5% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred onto nitrocellulose membranes (Thermo Scientific) or Immobilon-P polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were transferred to PVDF membranes (ThermoFisher) and blocked in TBS Odyssey Blocking Buffer (Li-Cor) ...
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bioRxiv - Cancer Biology 2021Quote: ... protein was transferred to nitrocellulose membrane (Thermo Fisher) in transfer buffer (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to PVDF membranes (#IB24001, ThermoFisher) on an iBlot™ device ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto PVDF membranes (Thermo Fisher). Membranes were blocked with a solution of 5% non-fat dry milk (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to nitrocellulose membranes (Invitrogen; IB23001) using the iBlot 2 System (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were transferred to nitrocellulose membranes (ThermoFisher Scientific) and immunoblotted with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... the proteins were applied to nitrocellulose membrane (Invitrogen). In order to positively identify the proteins ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... proteins were transferred onto PVDF membranes (Thermo Scientific), probed with antibodies ...
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bioRxiv - Synthetic Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (Thermo Fisher) and probed for EBNA1 (Merck ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (Fisher Scientific) using wet transfer (100V for 90 minutes on ice ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were transferred to PVDF membranes (Invitrogen IB24002) using the iBlot 2 Gel Transfer Device ...
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bioRxiv - Microbiology 2023Quote: ... proteins were blotted onto a PVDF membrane (Invitrogen) using an XCell II Blot Module system (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane (ThermoFisher) using the iBlot 2 dry transfer system (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were blotted onto PVDF membrane (Thermo Scientific) and probed with corresponding monoclonal (mAb ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were transferred to the nitrocellulose membrane (Invitrogen) by electroblotting ...
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bioRxiv - Microbiology 2023Quote: ... Protein was transferred onto nitrocellulose membranes (iBlot, Invitrogen) at 20 V for 7 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to PVDF membrane (Invitrogen, IB24002) and blocking was done with 5% non-fat milk for 1h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Microbiology 2020Quote: ... anti-NeuN and anti-SARS-CoV-2 spike protein antibodies (Thermo Fisher Scientific, Norcross, GA, USA) overnight at 4°C followed by incubation with Alexa Fluor 546- or Alexa Fluor 488-conjugated secondary antibody for 1 hr at room temperature [31,34] ...
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bioRxiv - Molecular Biology 2020Quote: Artificial codon optimized genes encoding SARS-CoV-2 nsp10 and nsp16 proteins were commercially synthesized (Invitrogen) and cloned in a pSUMO vector that encodes an N-terminal His8x-SUMO tag ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated SARS-CoV-2 S1 and RBD proteins were combined with fluorescent NeutrAvidin beads (Life Technologies) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Assays were performed in 24-well plates coated with Geltrex LDEV (lactose dehydrogenase elevating virus)-Free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher) at 75 µl/cm2 according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... E8 supplement ThermoFisher-A1517001) on tissue-culture dishes coated with 10 µg/cm2 of reduced Growth Factor Basement Membrane matrix (Geltrex, ThermoFisher A1413202 resuspended in basal medium DMEM/F12 Thermo Fisher 21331020) ...