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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Recombinant human IgE kappa L His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Dynabeads™ His-Tag Isolation and Pulldown magnetic bead solution (Thermo Fisher Scientific, United States) kit was used for the detection of the binding partner of OipA ...
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bioRxiv - Cell Biology 2024Quote: ... Western blotting was performed using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) and GST monoclonal mouse antibody (own antibody) ...
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bioRxiv - Molecular Biology 2020Quote: ... The total IgE level of individual sera was measured using an IgE mouse ELISA kit (Thermo Fisher Scientific, Waltham, USA), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... IgE Elisa Kit was purchased from Thermo Fisher Scientific.
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bioRxiv - Immunology 2022Quote: ... human recombinant Annexin V (5 μg/ml) (Invitrogen, #BMS306) or with the same amounts of their respective solvent (DMSO or H2O ...
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bioRxiv - Cancer Biology 2019Quote: ... Human recombinant bFGF and EGF were purchased from Invitrogen/Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml human recombinant EGF (Thermo Fisher) at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg/mL human recombinant insulin (Thermo Fisher Scientific), 20 μg/mL ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 ng/ml human recombinant FGF8 (Gibco, PHG0184). The medium was refreshed daily for 7–10 days as the cells aggregated to form spheroids.
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 ng/mL human recombinant FGF (Gibco, #AA10-155), 10 ng/mL heparin sulfate (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.01 mg/ml human recombinant insulin (Life Technologies). T-47D cells were cultured in RPMI-1640 Medium (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: Recombinant human Dynactin was expressed using Expi293F cells (Invitrogen) and Expi293 expression medium (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1pM Insulin Human Recombinant Zinc (Life Technologies 12585-014). For generating steatosis cells were supplemented oleic acid-albumin (Sigma-O3008 ...
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bioRxiv - Immunology 2020Quote: ... or 20 ng/ml recombinant human IL4 (Life Technologies), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... MA) and recombinant human insulin was obtained from Gibco.
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bioRxiv - Cancer Biology 2021Quote: ... Human recombinant insulin (10 μg ml−1, Gibco #12585014) was additionally included in the culture media for MCF7 ...
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bioRxiv - Microbiology 2022Quote: ... and 50 IU/mL recombinant human IL-2 (Gibco). IL-2 was also supplemented to each culture for the maintenance of the cells.
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/mL recombinant human EGF (#PHG0313, Gibco, USA), 20 % Knockout Serum Replacement (#10828028 ...
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bioRxiv - Physiology 2023Quote: ... hiPSCs were cultured on recombinant human vitronectin (rhVTN, ThermoFisher) in E8 Flex medium (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg/mL human recombinant insulin (Thermo Fisher Scientific), 1mM sodium pyruvate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 10 ng/mL recombinant human epidermal growth factor (Invitrogen), 0.1 μM Dibenzazepine (DBZ ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human thioredoxin 1 (hTrx) was purchased from ThermoFisher. The plasmid pET-TRSter for heterologous expression of human thioredoxin reductase (hTrxR ...
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bioRxiv - Cancer Biology 2022Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... on recombinant human Vitronectin (A14700, Thermo Fisher / Life Technologies)-coated 6-well plates ...
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bioRxiv - Developmental Biology 2023Quote: ... on recombinant human Vitronectin (A14700, Thermo Fisher / Life Technologies)-coated 6-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg/mL recombinant human insulin (Thermo Fisher Scientific), 0.55 µg/mL human transferrin (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... 50 ng/ml human recombinant EGF (10450-013; Gibco), 3.0% Noggin/R- Spondin-conditioned media ...
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bioRxiv - Genomics 2024Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), and 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Immunology 2020Quote: ... a history of peanut-induced reactions consistent with immediate hypersensitivity and peanut-specific serum IgE levels greater than 5 kU/L (ImmunoCAP; Thermo Fisher). Subjects who met these criteria then underwent a double-blind placebo-controlled food challenge to confirm peanut allergy ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR amplification was performed with Kappa Sybr qPCR mix or Kappa Probe qPCR mix using QuantStudio7 (Applied Biosystems). The mouse GAPDH gene was used as an endogenous control ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Immunology 2021Quote: ... The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ) (CaptureSelect; Thermo Fisher), and incubated 20min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Biophysics 2021Quote: ... and 6xHis tags Recombinant baculoviruses were generated using the Bac-to-Bac system (Thermo Fisher). High Five cells (Thermo Fisher B85502 ...
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bioRxiv - Microbiology 2020Quote: ... Total IgE was measured by ELISA using kit (Invitrogen) and performed according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... IgM and IgE with Mouse Uncoated ELISA kits (Invitrogen) according to the manufacturer’s instructions ...