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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Recombinant human IgE kappa L His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Systems Biology 2023Quote: ... and human IgE-PE (ThermoFisher). Antibody details are shown in Table S4 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... 294.4 ng/l human recombinant EGF (Gibco, 10450-013) and 300 µM of CaCl2 (Kanto Chemicals ...
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bioRxiv - Systems Biology 2020Quote: ... or human IgE-PE (clone BE5, Thermo Fisher) to measure isotype or IgG subclass antigen-specific antibodies ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Microbiology 2023Quote: ... HRP labelled goat anti-human IgE antibodies (Invitrogen, Waltham, MA) were used as the secondary antibodies for detection and the color wasa developed using TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315 ...
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Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Cell Biology 2020Quote: ... His-tags were removed with AcTEV protease (Invitrogen) overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Immunology 2024Quote: Dynabeads His-Tag Isolation and Pulldown beads (ThermoFisher) were washed once with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... anti-6*his tag (Thermo Fisher Scientific,11533923), anti-myc tag (Abcam ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were incubated 1 mg L-1 of anti-6X-His tag monoclonal antibody [HIS.H8] with an HRP conjugate (ThermoFisher) suspended in 10 mL 1X TBST for 0.5 hours ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
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bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
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bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
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bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Bioengineering 2023Quote: ... or anti-human CD68 (IgG3, kappa, PG-M1, ThermoFisher Scientific, MA, USA) antibodies were diluted 1:50 in PBS supplemented with 0.1% BSA and applied on the devices overnight at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...