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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: HEK293-FlpIn cells (ThermoFisher Scientific) were transfected with empty vector (negative control ...
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bioRxiv - Biochemistry 2022Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293-FT (Thermo Fisher Scientific) in DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293-F cells (ThermoFisher R79007) were transfected with the transfer plasmid and the packaging plasmids (Addgene plasmids #12263 and #8454 ...
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bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney (HEK293) and A549 cell lines were maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Lithuania) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated protein complex beads were digested into peptides using 500ng sequencing grade trypsin (Thermo Fisher Scientific) and incubated overnight at 37°C on a shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein extraction was done using PBS with 5 mM EDTA and protease phosphatase inhibitor complex (Thermofisher) by passing cells through a 24g needle ten times ...
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bioRxiv - Cancer Biology 2023Quote: ... The immunoprecipitated complex was then captured using a combination of Protein A and G Dynabeads (Invitrogen), washed and RNase A treated ...
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bioRxiv - Genomics 2024Quote: ... RNA-Protein complex was transferred to the Nylon membrane using the iBlot transfer system (ThermoFisher Scientific), and the probe signal was detected using a Chemiluminescent Nucleic acid detection module (#80880 ...
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bioRxiv - Cancer Biology 2019Quote: Human protein arrays (ProtoArrays V5.0, Life Technologies) kept at -20°C were equilibrated at 4 °C for 15 min and ...
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bioRxiv - Bioengineering 2020Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen), 0.178 mM Adenine (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... G-CSF Recombinant Human Protein (Thermo fisher), IP injection ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Cell Biology 2022Quote: ... PANC-1 WT and PANC-1 shTAK1 were cultured in DMEM (GIBCO) with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2019Quote: ... and HLA-DR/DP/DQ (CR3/43, ThermoFisher) antibody diluted 1:100 using SignalStain Diluent (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit polyclonal anti-HLA-B (Thermo Fisher Scientific), rabbit polyclonal anti-RPL7a (Cell Signaling) ...
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bioRxiv - Cancer Biology 2021Quote: ... HLA-A2 (141Pr, BB7.2, Thermo Fisher, MA1-80117)
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293 6E cells were used for transient production of these proteins following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection by centrifugation at 6,000 rpm for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... The human Dicer/TRBP enzyme complex was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: The protein lysate or exosomal protein from of WT H69 and ∆huPGRN-H69 cells were prepared using M-PER mammalian protein extraction reagent (ThermoFisher) or exosome protein isolation kit (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: Proteins from WT C57BL/6J testis were isolated utilizing the T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with Halt Protease Inhibitor Single-Use Cocktail EDTA-Free (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... before RP separation with a 60 min linear acetonitrile in water gradient of 2-25 or 35% (HLA-I/HLA-II and trypsin analyses) across a 75 µm × 50 cm PepMap RSLC C18 EasySpray column (Thermo Scientific) at a flow rate of 250 nl/min ...
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bioRxiv - Cell Biology 2022Quote: CHMP3 residues 1-150 wt or mutants were synthesized (ThermoFisher) and cloned into the pEGFP-N1 vector using restriction sites Xho1-HindIII ...
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bioRxiv - Neuroscience 2023Quote: ... CTB-Alexa647 (Molecular Probes, Invitrogen; 300 nL, 1% wt/vol) was delivered into Prh ...
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bioRxiv - Neuroscience 2023Quote: ... CTB-Alexa647 (Molecular Probes, Invitrogen; 300 nL, 1% wt/vol) was delivered into Prh ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa488 EGF complex (E13345, ThermoFisher) was used at 120 ng/mL ...
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bioRxiv - Bioengineering 2020Quote: ... Dex from the high Dex loading scaffolds (1% and 0.5% wt/wt) was measured by high-performance liquid chromatography (HPLC; Thermo Fisher) with an Acclaim 120 C18 3 µm 4.6×150 mm column ((Cat ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...
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bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney (HEK293) cells (RIKEN Cell Bank, Japan) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO, 11965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...