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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the human dynein complex was expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications1 ...
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bioRxiv - Immunology 2022Quote: HLA-DM and HLA-DR401 were expressed recombinantly in High Five insect cells (Thermo Fisher) using a baculovirus expression system ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Pathology 2019Quote: ... CSF-1 Recombinant Human Protein (25ng/mL, Thermo Fisher Scientific PHC9504), and Recombinant Human TGF-β1 (50ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... the purified protein complex was crosslinked with bis(sulfosuccinimidyl)suberate (BS3, Thermo Scientific), an amine-to-amine crosslinker ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were nucleofected with a preassembled gRNA and Cas9 protein RNP complex (ThermoFisher) using the 4D AMAXA nucleofector (Lonza) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resultant template-protein complex was pulled-down using a magnet stand (Invitrogen) and resuspended in NE buffer (17.5 ng/µL dI-dC ...
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bioRxiv - Plant Biology 2019Quote: ... The antibody-chromatin complex was captured using protein Dynabeads A or G (Invitrogen) for 2 h ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: HMC3 human microglia or HEK293 cells were transfected with Lipofectamine 3000 with Plus reagent (Invitrogen L3000001) per manufacturer instructions with 0.8 μL of Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Biochemistry 2021Quote: Human HEK293S GnTI- cells were cultured as suspension at 37°C in FreeStyle293 expression medium (Invitrogen) supplemented with 2% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney 293 cells (HEK293, ECACC) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney (HEK293) cells from ATCC were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco #12430-054) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293, HEK293A) cell lines were grown in Dulbeco’s Modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody-DNA complex was precipitated with protein A/G magnetic beads or protein A sepharose beads (Invitrogen). After washing ...
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bioRxiv - Genomics 2023Quote: ... The antibody-protein complex was recovered with protein A coupled to magnetic beads (Dynabeads, Invitrogen, Cat No. 10002D), followed by extensive washes with low salt immune complex wash buffer ...
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bioRxiv - Cell Biology 2021Quote: ... UQCRC1 (complex III) (1/1,000; Invitrogen, Waltham, Massachusetts, USA), SDHA (complex II ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Biochemistry 2021Quote: ... 2.25 mg/mL S protein and S protein-ACE2 complex (1:2.3 S protein trimer:ACE2 molar ratio) samples were vitrified using a Vitrobot Mark IV (Thermo Fisher Scientific) plunge freezing device ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-HLA-A03 (ThermoFisher Scientific, GAP.A3), and anti-HLA-B27 (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... with detection of the complex with goat anti–human IgG Fab (200 µg/well; Invitrogen, #31122).
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bioRxiv - Molecular Biology 2019Quote: ... Protein was purified using His-Pur Ni-NTA Resin (Thermo Scientific), equilibrated using 2 bed volumes of PBS pH=7.4 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Biochemistry 2024Quote: ... C2C12 cells transfected with EGFP-HRas WT/C181S/C184S in 35mm dishes were treated with 15d-PGJ2-Biotin (5 µM) in DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin-streptomycin-Glutamine (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... the complex was pulled down by magnetic beads coated with protein-G (88847, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... the co-immunoprecipitated protein complex was eluted with 4X LDS sample buffer (NP0007, Invitrogen). The samples were analyzed with the immunoblotting technique (see below).
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bioRxiv - Microbiology 2021Quote: ... Human alpha-1 PDX (alpha1-PDX) recombinant protein was purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5 μg of FGFb Recombinant Human Protein and 1% Gluta-MaxTM (Gibco) in flasks coated with Matrigel Matrix Basement Membrane (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 ng/mL Human TGF-β 1 recombinant protein (Cat# PHG9204, Gibco) and 1 μg/mL L-Ascorbic acid 2-phosphate (Cat# A8960 ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody-protein-chromatin complex was retrieved by adding 25μl pre-washed Protein G Dynabeads (Thermo Fisher Scientific, 10004D). Immunoprecipitated chromatin DNA was de-crosslinked by 65°C heating overnight using elution buffer (1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA-protein-antibody complex was captured using protein A/G magnetic beads (Thermo Fisher Scientific, 10002D and 10004D). Magnetic beads were washed with low salt buffer (1X PBS ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from HEK293 cells and human brain prefrontal cortex were isolated with the TRIzol reagent (Ambion) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: Human Embryonic Kidney 293 cells (HEK293, ATCC CRL-1573) were grown under standard conditions in DMEM (Gibco) supplemented with 10% FBS (BioConcept) ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: HEK293 and commercially available Flp-In T-REx HEK293 cells (Invitrogen; R78007) were cultured in DMEM (GIBCO ...
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bioRxiv - Biochemistry 2020Quote: ... Uncleaved His-tagged proteins were removed by incubation of the sample with 1 ml HisPur™ (Thermo Fisher) nickel resin for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Genetics 2023Quote: HEK293 cell protein was extracted with 0.3 mL of RIPA buffer (ThermoFisher, Cat. No. 89900) containing Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293S MGAT−/− cells were maintained in a 1:1 ratio of Freestyle (ThermoFisher) and Ex-Cell (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells (ThermoFisher Scientific) were cultured in DMEM/F-12 ...