Labshake search
Citations for Thermo Fisher :
201 - 250 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... 2 μl Maxima H- RTase (EP0751, Thermofisher), 4 μl Actinomycin D (500ng/μl ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with RNase H inhibitor (#AM2682, Ambion).
-
bioRxiv - Genomics 2022Quote: ... 0.03 U RNase H (Invitrogen, 18021-071)) was added to each well and the plates were incubated at 16°C for 2 hours ...
-
bioRxiv - Microbiology 2022Quote: ... RevertAid H Minus Reverse Transcriptase (Thermo Scientific) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2.5 U of RNase H (Thermo Scientific) was then added and the reaction incubated for 20 min at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... A Maxima H Minus RT (ThermoFisher EP0752) reaction was setup by mixing 40 μl of the T7 reaction with 30 μl 100 uM forward B primer ...
-
bioRxiv - Immunology 2022Quote: ... SuperScript IV RNase H Reverse Transcriptase (Invitrogen) was used to synthesize first-strand cDNA with random primer from 1 mg of total RNA at 50 °C for 50 min ...
-
bioRxiv - Immunology 2022Quote: ... and stained with H&E (Thermo Scientific) following standard histological procedures ...
-
bioRxiv - Genomics 2020Quote: ... and 0.11uL of Maximus H-RT (ThermoFisher). For samples from ring gates we added 17.9uL of RT Master Mix (in the same proportions ...
-
bioRxiv - Cell Biology 2020Quote: ... 15 U/μl Maxima H-RT (ThermoFisher), 1.65 U/μl RNasin (Promega) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1x Maxima H-RT buffer (Thermo Scientific) or 1x Kappa HiFi PCR buffer (Kapa Biosystems) ...
-
bioRxiv - Genomics 2020Quote: ... Maxima H Minus RT (Thermo Fisher Scientific) followed by 15 cycles of PCR using Takara LA Taq (Clontech ...
-
bioRxiv - Immunology 2021Quote: ... PE anti-H-2Db (ThermoFisher, Cat. # A15443), PerCP anti-Ly6C (BioLegend ...
-
bioRxiv - Immunology 2021Quote: ... Maxima H- minus (ThermoFisher, cat. no. EP0751) was used for reverse-transcription with template switching ...
-
bioRxiv - Immunology 2022Quote: ... SuperScript IV RNase H Reverse Transcriptase (Invitrogen) was used to synthesize first-strand cDNA with random primer from 1mg of total RNA at 50 °C for 50 min ...
-
bioRxiv - Genomics 2022Quote: ... 1μL of RNase H (Ambion, Cat # AM2293) was incubated with the mixture at 37°C for 20 minutes ...
-
bioRxiv - Pathology 2022Quote: ... or Maxima H minus (ThermoFisher, Waltham, Massachusetts) reverse transcriptase according to manufacturer’sinstructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Maxima H minus Reverse Transcriptase (Thermo Fisher) was used instead of SMARTScribe II Reverse Transcriptase ...
-
bioRxiv - Neuroscience 2023Quote: ... Maxima H Minus Reverse Transcriptase (ThermoFisher, EP0753) and oligo dT were used for cDNA synthesis ...
-
bioRxiv - Immunology 2024Quote: ... anti-chicken IgY (H+L) AF546 (Invitrogen) and anti-mouse IgG (H+L ...
-
bioRxiv - Bioengineering 2024Quote: ... diluted in DMEM-H (Gibco #11965-092). The inflated piece of lung was then cooled to 4°C for at least 30 minutes to solidify the agarose ...
-
bioRxiv - Bioengineering 2024Quote: ... high glucose (DMEM-H; Gibco #11965-092), was dispensed into the plate at 3 mL per well ...
-
bioRxiv - Biochemistry 2023Quote: ... RNase H (8 U; Invitrogen 18021-014), DNase I (5 U ...
-
bioRxiv - Plant Biology 2023Quote: ... or Maxima H Minus (Thermo Fisher Scientific) reverse transcriptase according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: Hematoxylin and Eosin (H & E) staining (Thermofisher) was performed according to instructions from the manufacturer for histological analysis and cerebellar area measurements of adult sections.
-
bioRxiv - Immunology 2023Quote: ... SuperScript IV RNase H Reverse Transcriptase (Invitrogen) was used to synthesize first-strand cDNA with random primer from 1mg of total RNA at 50 °C for 50 min ...
-
bioRxiv - Immunology 2024Quote: ... 1X Maxima H RT buffer (Thermo Scientific), 5 mM DTT (Thermo Scientific) ...
-
bioRxiv - Bioengineering 2024Quote: ... 2.5% FBS (#FB5001-H, Thermo Fisher Scientific), 1X Insulin-Transferrin-Selenium X (#51500-056 ...
-
bioRxiv - Biochemistry 2024Quote: ... for 4 h in OptiMEM (ThermoFisher, 31985062) and 1.5 μl 1 μg/μl PEI (Sigma ...
-
bioRxiv - Cancer Biology 2020Quote: ... Tissue was embedded in Tissue Freezing Medium (General Data, Cincinnati, OH) sectioned at 10-16 μm on a Shandon FE Cryostat (Thermo Fisher scientific, Waltham, MA). Slides were stained with Hematoxylin and Eosin (all reagents from VWR ...
-
bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... Formazan crystals formed were dissolved by adding isopropanol (100 µl) and absorbance was measured in a microplate reader (MultiSkan Go, Thermo Fisher Scientific, OH, USA) at 570 nm ...
-
bioRxiv - Neuroscience 2020Quote: ... and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L) Alexa Fluor 594 (cat# A-21207 ...
-
bioRxiv - Immunology 2022Quote: ... cells at 30 h (CD8+T) or 48 h (CD4+ T) after stimulation were permeabilized using Cytofix/Cytoperm (Invitrogen) and stained with a mixture of anti-Ki-67 antibody and NucSpot Far-Red for 15 min at room temperature in the dark ...
-
bioRxiv - Microbiology 2022Quote: ... interferon-β treated (250 U/ml for 8 h) or infected with influenza WSN (MOI 0.02 for 24 h) using TRIzol (Invitrogen). Biologic triplicate sample RNA was sent for library preparation and paired-end RNA-seq by Novogene (BioProject PRJNA667475) ...
-
bioRxiv - Microbiology 2022Quote: ... for 1 h and incubated in primary antibodies (S3 Table) overnight and in secondary antibodies for 1 h (Invitrogen) in the dark ...
-
bioRxiv - Cell Biology 2023Quote: ... The siRNA-mediated knockdown of proteins was performed for 144 h or 168 h using Lipofectamine RNAiMAX (Invitrogen, 13778150) diluted in Opti-MEM (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... for 1 h at room temperature [anti-rabbit secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 488), anti-rat 568 secondary antibody (H+L ...
-
bioRxiv - Developmental Biology 2021Quote: ... N/D and N/D/S line ESCs were dislodged with 0.025% trypsin/1% EDTA (Gibco), dissociated ...
-
bioRxiv - Developmental Biology 2021Quote: siRNAs targeting human CDK12 (Dharmacon D-004031-01 and D-004031-02) CDK13 (Life Technologies s16398) or control siRNAs (final concentration 10 nM ...
-
bioRxiv - Cancer Biology 2024Quote: ... IL13 blocking antibody (R&D) or isotype control (R&D) were dissolved in DPBS (Thermo Fisher) and administered intraperitoneally every other day at 1 mg/kg for a period of 8 wks ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products that contained a single band of the expected size were purified using ExoSAP-IT (USB Corporation, Cleveland, OH, USA) and directly sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) with BigDye™ Terminator v ...
-
bioRxiv - Microbiology 2020Quote: ... Broth micro-dilution antifungal susceptibility testing was performed in accordance with Reference Method M60 of the Clinical and Laboratory Standards Institute using TREK frozen broth microdilution panels (catalog number CML2FCAN; Thermo Fisher Scientific, Marietta, OH, USA) for FLC ...
-
bioRxiv - Microbiology 2023Quote: ... An in-line electrolytic suppressor was employed to remove OH− ions and cations from the post-column eluent prior to delivery to the MS system electrospray ion source (Thermo Fisher Scientific Dionex AERS 500).
-
bioRxiv - Cancer Biology 2024Quote: RNA was isolated from VCaP and LNCaP cells post AD with or without DHT treatments with TRIsure (Meridian Bioscience, Cincinnati, OH, USA) or TRIzol reagent (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... or 5%) and quercetin (0.1 μM or 100 μM) at 30 °C and 200 rpm in an orbital shaker (Thermo Scientific, MaxQ 600, Marietta, OH, USA). Cells in the mid-log phase (OD600 ~ 0.5 ...
-
bioRxiv - Microbiology 2024Quote: ... qPCR was conducted in 384 well plates using SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience, Cincinnati, OH, USA) in a QuantStudio 5 qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program ran according to enzyme requirements for 40 cycles ...
-
bioRxiv - Immunology 2024Quote: ... ELISpot specific for IFN-γ and TNF-α was carried out using diluted whole blood in CTL-Test™ media (Cellular Technologies Ltd, CTL, Cleveland, OH, USA) supplemented with 2mM L-Glutamine (ThermoFisher Scientific, Waltham, MA, USA). Immunomodulatory agents included LPS (ENZO Life Sciences ...
-
bioRxiv - Plant Biology 2020Quote: ... cloned into pENTR/D-TOPO (Invitrogen), and subcloned into pTRV2-GW through an LR reaction (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... and poly-D-Lysine (A3890401, Gibco).